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J Clin Invest. 1986 October; 78(4): 928–933. doi: 10.1172/JCI112682. | PMCID: PMC423721 |
Palmitate and glycerol kinetics during brief starvation in normal weight young adult and elderly subjects. S Klein, V R Young, G L Blackburn, B R Bistrian, and R R Wolfe Abstract Data obtained in vitro suggest that the ability to mobilize fat decreases with age. We determined lipolytic rates in vivo in normal weight young adult (22-33 yr) and elderly (65-77 yr) subjects using a simultaneous infusion of [1,2-13C2]palmitate and [2H5]glycerol. The subjects were studied after a 12-h fast and again after 60-82 h of fasting. When lipolysis was expressed per unit of adipose tissue the values for the young adults were more than double those for the elderly (P less than 0.05). However, the amount of body fat in the elderly was twice that of the young adults, so that lipolysis per unit of body weight was similar in both groups. These results demonstrate that lipolysis per unit of adipose tissue is lower in elderly subjects. This may be due to their increase in body fat, however, since the total amount of potential energy mobilized from adipose tissue was similar to that of the young adults. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. These references are in PubMed. This may not be the complete list of references from this article. - Masoro EJ, Bertrand H, Liepa G, Yu BP. Analysis and exploration of age-related changes in mammalian structure and function. Fed Proc. 1979 May;38(6):1956–1961. [PubMed]
- Feldman RD, Limbird LE, Nadeau J, Robertson D, Wood AJ. Alterations in leukocyte beta-receptor affinity with aging. A potential explanation for altered beta-adrenergic sensitivity in the elderly. N Engl J Med. 1984 Mar 29;310(13):815–819. [PubMed]
- Lakatta EG. Age-related alterations in the cardiovascular response to adrenergic mediated stress. Fed Proc. 1980 Dec;39(14):3173–3177. [PubMed]
- Abrass IB, Davis JL, Scarpace PJ. Isoproterenol responsiveness and myocardial beta-adrenergic receptors in young and old rats. J Gerontol. 1982 Mar;37(2):156–160. [PubMed]
- Nakano J, Gin AC, Ishii T. Effect of age on norepinephrine-, ACTH-, theophylline- and dibutryl cyclic AMP-induced lipolysis in isolated rat fat cells. J Gerontol. 1971 Jan;26(1):8–12. [PubMed]
- Yu BP, Bertrand HA, Masoro EJ. Nutrition-aging influence of catecholamine-promoted lipolysis. Metabolism. 1980 May;29(5):438–444. [PubMed]
- Dax EM, Partilla JS, Gregerman RI. Mechanism of the age-related decrease of epinephrine-stimulated lipolysis in isolated rat adipocytes: beta-adrenergic receptor binding, adenylate cyclase activity, and cyclic AMP accumulation. J Lipid Res. 1981 Aug;22(6):934–943. [PubMed]
- Hoffman BB, Chang H, Farahbakhsh ZT, Reaven GM. Age-related decrement in hormone-stimulated lipolysis. Am J Physiol. 1984 Dec;247(6 Pt 1):E772–E777. [PubMed]
- Cooper B, Gregerman RI. Hormone-sensitive fat cell adenylate cyclase in the rat. Influences of growth, cell size, and aging. J Clin Invest. 1976 Jan;57(1):161–168. [PMC free article] [PubMed]
- Cooper B, Gregerman RI. Hormone-sensitive fat cell adenylate cyclase in the rat. Influences of growth, cell size, and aging. J Clin Invest. 1976 Jan;57(1):161–168. [PMC free article] [PubMed]
- Forn J, Schonhofer PS, Skidmore IF, Krishna G. Effect of aging on the adenyl cyclase and phosphodiesterase activity of isolated fat cells of rat. Biochim Biophys Acta. 1970 May 12;208(2):304–309. [PubMed]
- Young JB, Landsberg L. Sympathoadrenal activity in fasting pregnant rats. Dissociation of adrenal medullary and sympathetic nervous system responses. J Clin Invest. 1979 Jul;64(1):109–116. [PMC free article] [PubMed]
- Young James B, Landsberg Lewis. Sympathoadrenal Activity in Fasting Pregnant Rats: DISSOCIATION OF ADRENAL MEDULLARY AND SYMPATHETIC NERVOUS SYSTEM RESPONSES. J Clin Invest. 1979 Jul;64(1):109–116. [PMC free article] [PubMed]
- Palmblad J, Levi L, Burger A, Melander A, Westgren U, von Schenck H, Skude G. Effects of total energy withdrawal (fasting) on thelevels of growth hormone, thyrotropin, cortisol, adrenaline, noradrenaline, T4, T3, and rT3 in healthy males. Acta Med Scand. 1977 Jan;201(1-2):15–22. [PubMed]
- Young JB, Rosa RM, Landsberg L. Dissociation of sympathetic nervous system and adrenal medullary responses. Am J Physiol. 1984 Jul;247(1 Pt 1):E35–E40. [PubMed]
- Zapf J, Waldvogel M, Froesch ER. Increased sensitivity of rat adipose tissue to the lipolytic action of epinephrine during fasting and its reversal during re-feeding. FEBS Lett. 1977 Apr 1;76(1):135–138. [PubMed]
- Dax EM, Partilla JS, Gregerman RI. Increased sensitivity to epinephrine stimulated lipolysis during starvation: tighter coupling of the adenylate cyclase complex. Biochem Biophys Res Commun. 1981 Aug 31;101(4):1186–1192. [PubMed]
- McGuire EA, Helderman JH, Tobin JD, Andres R, Berman M. Effects of arterial versus venous sampling on analysis of glucose kinetics in man. J Appl Physiol. 1976 Oct;41(4):565–573. [PubMed]
- Wolfe RR, Evans JE, Mullany CJ, Burke JF. Measurement of plasma free fatty acid turnover and oxidation using [1-13C]palmitic acid. Biomed Mass Spectrom. 1980 Apr;7(4):168–171. [PubMed]
- Bortz WM, Paul P, Haff AC, Holmes WL. Glycerol turnover and oxidation in man. J Clin Invest. 1972 Jun;51(6):1537–1546. [PMC free article] [PubMed]
- Bortz Walter M, Paul Pavle, Haff Agnes C, Holmes William L. Glycerol turnover and oxidation in man. J Clin Invest. 1972 Jun;51(6):1537–1546. [PMC free article] [PubMed]
- Davis GC, Kissinger PT, Shoup RE. Strategies for determination of serum or plasma norepinephrine by reverse-phase liquid chromatography. Anal Chem. 1981 Feb;53(2):156–159. [PubMed]
- YALOW RS, BERSON SA. Immunoassay of endogenous plasma insulin in man. J Clin Invest. 1960 Jul;39:1157–1175. [PMC free article] [PubMed]
- Yalow Rosalyn S, Berson Solomon A. IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MAN. J Clin Invest. 1960 Jul;39(7):1157–1175. [PMC free article] [PubMed]
- Hales CN, Randle PJ. Immunoassay of insulin with insulin-antibody precipitate. Biochem J. 1963 Jul;88(1):137–146. [PMC free article] [PubMed]
- HALES CN, RANDLE PJ. Immunoassay of insulin with insulin-antibody precipitate. Biochem J. 1963 Jul;88:137–146. [PMC free article] [PubMed]
- Schade DS, Eaton RP. The regulation of plasma ketone body concentration by counterregulatory hormones in man. Diabetes. 1977 Oct;26(10):989–996. [PubMed]
- McDonald-Gibson RG, Young M. The use of an automatic solids injection system for quantitative determination of plasma long chain non-esterified fatty acids by gas-liquid chromatography. Clin Chim Acta. 1974 May 31;53(1):117–126. [PubMed]
- Schoeller DA, van Santen E, Peterson DW, Dietz W, Jaspan J, Klein PD. Total body water measurement in humans with 18O and 2H labeled water. Am J Clin Nutr. 1980 Dec;33(12):2686–2693. [PubMed]
- STEELE R. Influences of glucose loading and of injected insulin on hepatic glucose output. Ann N Y Acad Sci. 1959 Sep 25;82:420–430. [PubMed]
- Spitzer JJ, Gold M. Studies on the metabolism of free fatty acids in diabetic and fasting dogs. Ann N Y Acad Sci. 1965 Oct 8;131(1):235–249. [PubMed]
- Hagenfeldt L, Wahren J, Pernow B, Räf L. Uptake of individual free fatty acids by skeletal muscle and liver in man. J Clin Invest. 1972 Sep;51(9):2324–2330. [PMC free article] [PubMed]
- Hagenfeldt Lars, Wahren John, Pernow Bengt, Räf Lars. Uptake of individual free fatty acids by skeletal muscle and liver in man. J Clin Invest. 1972 Sep;51(9):2324–2330. [PMC free article] [PubMed]
- Lesser GT, Deutsch S, Markofsky J. Use of independent measurement of body fat to evaluate overweight and underweight. Metabolism. 1971 Aug;20(8):792–804. [PubMed]
- Lilavivathana U, Campbell RG, Brodows RG. Control of insulin secretion during fasting in man. Metabolism. 1978 Jul;27(7):815–821. [PubMed]
- Aguilar-Parada E, Eisentraut AM, Unger RH. Effects of starvation on plasma pancreatic glucagon in normal man. Diabetes. 1969 Nov;18(11):717–723. [PubMed]
- ARMSTRONG DT, STEELE R, ALTSZULER N, DUNN A, BISHOP JS, DE BODO RC. Regulation of plasma free fatty acid turnover. Am J Physiol. 1961 Jul;201:9–15. [PubMed]
- Issekutz B, Jr, Paul P, Miller HI, Bortz WM. Oxidation of plasma FFA in lean and obese humans. Metabolism. 1968 Jan;17(1):62–73. [PubMed]
- Cahill GF, Jr, Herrera MG, Morgan AP, Soeldner JS, Steinke J, Levy PL, Reichard GA, Jr, Kipnis DM. Hormone-fuel interrelationships during fasting. J Clin Invest. 1966 Nov;45(11):1751–1769. [PMC free article] [PubMed]
- Cahill GF, Jr, Herrera MG, Morgan AP, Soeldner JS, Steinke J, Levy PL, Reichard GA, Jr, Kipnis DM. Hormone-fuel interrelationships during fasting. J Clin Invest. 1966 Nov;45(11):1751–1769. [PMC free article] [PubMed]
- Cahill GF., Jr Starvation in man. Clin Endocrinol Metab. 1976 Jul;5(2):397–415. [PubMed]
- Clutter WE, Bier DM, Shah SD, Cryer PE. Epinephrine plasma metabolic clearance rates and physiologic thresholds for metabolic and hemodynamic actions in man. J Clin Invest. 1980 Jul;66(1):94–101. [PMC free article] [PubMed]
- Clutter WE, Bier DM, Shah SD, Cryer PE. Epinephrine plasma metabolic clearance rates and physiologic thresholds for metabolic and hemodynamic actions in man. J Clin Invest. 1980 Jul;66(1):94–101. [PMC free article] [PubMed]
- Galster AD, Clutter WE, Cryer PE, Collins JA, Bier DM. Epinephrine plasma thresholds for lipolytic effects in man: measurements of fatty acid transport with [l-13C]palmitic acid. J Clin Invest. 1981 Jun;67(6):1729–1738. [PMC free article] [PubMed]
- Galster AD, Clutter WE, Cryer PE, Collins JA, Bier DM. Epinephrine plasma thresholds for lipolytic effects in man: measurements of fatty acid transport with [l-13C]palmitic acid. J Clin Invest. 1981 Jun;67(6):1729–1738. [PMC free article] [PubMed]
- Gorman RR, Tepperman HM, Tepperman J. Effects of starvation, refeeding, and fat feeding on adipocyte ghost adenyl cyclase activity. J Lipid Res. 1972 Mar;13(2):276–280. [PubMed]
- Zapf J, Feuerlein D, Waldvogel M, Froesch ER. Increased sensitivity of diabetic rat adipose tissue towards the lipolytic action of epinephrine. Diabetologia. 1975 Dec;11(6):509–516. [PubMed]
- Landsberg L, Young JB. Fasting, feeding and regulation of the sympathetic nervous system. N Engl J Med. 1978 Jun 8;298(23):1295–1301. [PubMed]
- Young JB, Landsberg L. Suppression of sympathetic nervous system during fasting. Science. 1977 Jun 24;196(4297):1473–1475. [PubMed]
- O'Dea K, Esler M, Leonard P, Stockigt JR, Nestel P. Noradrenaline turnover during under- and over-eating in normal weight subjects. Metabolism. 1982 Sep;31(9):896–899. [PubMed]
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