• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Sep 1972; 6(3): 211–217.
PMCID: PMC422518

Effect of Catecholamines on the Bactericidal Activity of Polymorphonuclear Leukocytes 1

Abstract

Epinephrine, norepinephrine, and dihydroxyphenylalanine at 8 mm concentrations prevented iodination of zymosan by intact neutrophils and decarboxylation of l-alanine by leukocyte sonic extracts. The same concentration of epinephrine also reduced bactericidal activity of the leukocyte against Staphylococcus aureus, Enterobacter cloacae, and Proteus rettgeri without decreasing phagocytosis of bacteria. Spectral studies indicated that epinephrine interferes with the myeloperoxidase-mediated reactions by competing for available H2O2 via its enzymatic oxidation to adrenochrome. These findings support a mechanism in which H2O2 plays an important role in the bactericidal activity of the leukocyte.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (983K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cooper MR, DeChatelet LR, McCall CE, LaVia MF, Spurr CL, Baehner RL. Leucocyte G.-6-P.D. deficiency. Lancet. 1970 Jul 11;1(7663):110–110. [PubMed]
  • DeChatelet LR, Cooper MR. A modified procedure for the determination of leukocyte alkaline phosphatase. Biochem Med. 1970 Aug;4(1):61–68. [PubMed]
  • Dechatelet LR, Cooper MR, McCall CE. Dissociation by colchicine of the hexose monophosphate shunt activation from the bactericidal activity of the leukocyte. Infect Immun. 1971 Jan;3(1):66–72. [PMC free article] [PubMed]
  • DeChatelet LR, Cooper MR, McCall CE. Stimulation of the hexose monophosphate shunt in human neutrophils by ascorbic acid: mechanism of action. Antimicrob Agents Chemother. 1972 Jan;1(1):12–16. [PMC free article] [PubMed]
  • Green DE, Richter D. Adrenaline and adrenochrome. Biochem J. 1937 Apr;31(4):596–616. [PMC free article] [PubMed]
  • HIRSCH JG. Cinemicrophotographic observations on granule lysis in polymorphonuclear leucocytes during phagocytosis. J Exp Med. 1962 Dec 1;116:827–834. [PMC free article] [PubMed]
  • Holmes B, Page AR, Good RA. Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function. J Clin Invest. 1967 Sep;46(9):1422–1432. [PMC free article] [PubMed]
  • Karnovsky ML. The metabolism of leukocytes. Semin Hematol. 1968 Apr;5(2):156–165. [PubMed]
  • Klebanoff SJ. Iodination of bacteria: a bactericidal mechanism. J Exp Med. 1967 Dec 1;126(6):1063–1078. [PMC free article] [PubMed]
  • Lehrer RI, Cline MJ. Interaction of Candida albicans with human leukocytes and serum. J Bacteriol. 1969 Jun;98(3):996–1004. [PMC free article] [PubMed]
  • McCall CE, DeChatelet LR, Cooper MR, Ashburn P. The effects of ascorbic acid on bactericidal mechanisms of neutrophils. J Infect Dis. 1971 Aug;124(2):194–198. [PubMed]
  • Miller TE. Killing and lysis of gram-negative bacteria through the synergistic effect of hydrogen peroxide, ascorbic acid, and lysozyme. J Bacteriol. 1969 Jun;98(3):949–955. [PMC free article] [PubMed]
  • Paul BB, Jacobs AA, Strauss RR, Sbarra AJ. Role of the Phagocyte in Host-Parasite Interactions XXIV. Aldehyde Generation by the Myeloperoxidase-H(2)O(2)-Chloride Antimicrobial System: a Possible In Vivo Mechanism of Action. Infect Immun. 1970 Oct;2(4):414–418. [PMC free article] [PubMed]
  • Pincus SH, Klebanoff SJ. Quantitative leukocyte iodination. N Engl J Med. 1971 Apr 8;284(14):744–750. [PubMed]
  • Qualliotine D, DeChatelet LR, McCall CE, Cooper MR. Stimulation of oxidative metabolism in polymorphonuclear leukocytes by catecholamines. J Reticuloendothel Soc. 1972 Mar;11(3):263–276. [PubMed]
  • REPASKE R. Lysis of gram-negative bacteria by lysozyme. Biochim Biophys Acta. 1956 Oct;22(1):189–191. [PubMed]
  • SBARRA AJ, KARNOVSKY ML. The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes. J Biol Chem. 1959 Jun;234(6):1355–1362. [PubMed]
  • Strauss RR, Paul BB, Jacobs AA, Sbarra AJ. Role of the phagocyte in host-parasite interactions. XXII. H2O2-dependent decarbosylation and deamination by myeloperoxidase and its relationship to antimicrobial activity. J Reticuloendothel Soc. 1970 Jun;7(6):754–761. [PubMed]
  • WALAAS E, WALAAS O. Oxidation of reduced phosphopyridine nucleotides by p-phenylenediamines, catecholamines and serotonin in the presence of ceruloplasmin. Arch Biochem Biophys. 1961 Oct;95:151–162. [PubMed]
  • ZEYA HI, SPITZNAGEL JK. ANTIBACTERIAL AND ENZYMIC BASIC PROTEINS FROM LEUKOCYTE LYSOSOMES: SEPARATION AND IDENTIFICATION. Science. 1963 Nov 22;142(3595):1085–1087. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...