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Journal List > Infect Immun > v.3(2); Feb 1971

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Infect Immun. 1971 February; 3(2): 355–357.
PMCID: PMC416155
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Rapid Fluorescent-Antibody Conjugation Procedure
M. Peacock, W. Burgdorfer, and R. A. Ormsbee
U. S. Department of Health, Education, and Welfare, Public Health Service, Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840
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Abstract
This note describes a technique developed for rapid preparation of specific and brilliant conjugates for direct staining of rickettsial agents in yolk-sac, tick, and vertebrate tissues.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • BAUMSTARK JS, LAFFIN RJ, BARDAWIL WA. A PREPARATIVE METHOD FOR THE SEPARATION OF 7S GAMMA GLOBULIN FROM HUMAN SERUM. Arch Biochem Biophys. 1964 Dec;108:514–522. [PubMed]
  • COONS AH. Fluorescent antibody methods. Gen Cytochem Methods. 1958;1:399–422. [PubMed]
  • CURTAIN CC. The chromatographic purification of fluorescein-antibody. J Histochem Cytochem. 1961 Sep;9:484–486. [PubMed]
  • Fiset P, Ormsbee RA, Silberman R, Peacock M, Spielman SH. A microagglutination technique for detection and measurement of rickettsial antibodies. Acta Virol. 1969 Jan;13(1):60–66. [PubMed]
  • LIU C. The use of fluorescent antibody in the diagnosis and study of viral and rickettsial infections. Ergeb Mikrobiol Immunitatsforsch Exp Ther. 1960;33:242–258. [PubMed]
  • MCKINNEY RM, SPILLANE JT, PEARCE GW. FACTORS AFFECTING THE RATE OF REACTION OF FLUORESCEIN ISOTHIOCYANATE WITH SERUM PROTEINS. J Immunol. 1964 Aug;93:232–242. [PubMed]
  • Oldstone MB, Dixon FJ. Direct immunofluorescent tissue culture assay for lymphocytic choriomeningitis virus. J Immunol. 1968 May;100(5):1135–1138. [PubMed]
  • RINDERKNECHT H. Ultra-rapid fluorescent labelling of proteins. Nature. 1962 Jan 13;193:167–168. [PubMed]
  • The TH, Feltkamp TE. Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation. Immunology. 1970 Jun;18(6):865–873. [PMC free article] [PubMed]
  • The TH, Feltkamp TEW. Conjugation of fluorescein isothiocyanate to antibodies: I. Experiments on the conditions of conjugation. Immunology. 1970 Jun;18(6):865–873. [PMC free article] [PubMed]
  • The TH, Feltkamp TEW. Conjugation of fluorescein isothiocyanate to antibodies: II. A reproducible method. Immunology. 1970 Jun;18(6):875–881. [PMC free article] [PubMed]
  • The TH, Feltkamp TE. Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method. Immunology. 1970 Jun;18(6):875–881. [PMC free article] [PubMed]
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