• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Nov 1970; 2(5): 617–622.
PMCID: PMC416059

Antibody in Cattle Experimentally Infected with Contagious Bovine Pleuropneumonia

Abstract

Antibody responses in cattle experimentally infected with contagious bovine pleuropneumonia were assayed by the complement fixation (CF), plate agglutination (PA), and agar-gel diffusion precipitin (AGDP) tests. Sera were also fractionated by sucrose gradient centrifugation, by chromatography on diethylaminoethyl (DEAE)-cellulose columns, and by starch-block electrophoresis. Serum fractions were assayed for antibody by the CF and PA tests. Antibody was detected by the CF and PA tests from 7 days postinoculation (DPI) throughout the test period of 154 days. The AGDP test was the least sensitive and was negative after 28 DPI. By sucrose gradient centrifugal analysis, only 19S antibody could be detected in the 7 DPI sera, but 19 and 7S antibodies were found in the 14, 21, 28, 35, and 42 DPI sera. Only 7S antibody was found in sera subsequently collected until 154 DPI at the conclusion of the experiment. Results of chromatographic fractionation of sera on DEAE-cellulose columns showed that most of the CF antibody was adsorbed when 0.0175 m phosphate buffer (pH 6.3) was used for elution of proteins. Plate agglutination antibody activity was found in both immunoglobulin (Ig)G and IgM fractions from 7 to 42 DPI and only in the IgG fraction thereafter. By starch-block electrophoresis, the 14 DPI antibody was of beta mobility (IgM), whereas at 21 DPI the antibody mobility was gamma-beta. In the 28 and later DPI sera, CF antibody had gamma mobility.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (966K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Morgan WJ. The serological diagnosis of bovine brucellosis. Vet Rec. 1967 May 27;80(21):612–620. [PubMed]
  • EDELMAN GM, KUNKEL HG, FRANKLIN EC. Interaction of the rheumatoid factor with antigen-antibody complexes and aggregated gamma globulin. J Exp Med. 1958 Jul 1;108(1):105–120. [PMC free article] [PubMed]
  • Edsall G. Principles of active immunization. Annu Rev Med. 1966;17:39–48. [PubMed]
  • KUNKEL HG. Zone electrophoresis. Methods Biochem Anal. 1954;1:141–170. [PubMed]
  • LEVY HB, SOBER HA. A simple chromatographic method for preparation of gamma globulin. Proc Soc Exp Biol Med. 1960 Jan;103:250–252. [PubMed]
  • Murphy FA, Osebold JW, Aalund O. Physical heterogeneity of bovine gamma-globulins: characterization of gamma-M and gamma-G globulins. Arch Biochem Biophys. 1965 Oct;112(1):126–136. [PubMed]
  • OSLER AG, STRAUSS JH, MAYER MM. Diagnostic complement fixation. I. A method. Am J Syph Gonorrhea Vener Dis. 1952 Mar;36(2):140–153. [PubMed]
  • Pike RM. Antibody heterogeneity and serological reactions. Bacteriol Rev. 1967 Jun;31(2):157–174. [PMC free article] [PubMed]
  • Rice CE, Alexander DC, Barrett BB. Chromatographic studies of sera from calves vaccinated with brucella abortus, strain 19. Can J Comp Med Vet Sci. 1967 May;31(5):114–121. [PMC free article] [PubMed]
  • SCHEIDEGGER JJ. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed]
  • Shifrine M, Domermuth CH. Contagious bovine pleuropneumonia in wildlife. Bull Epizoot Dis Afr. 1967 Dec;15(4):319–322. [PubMed]
  • Shifrine M, Moulton JE. Infection of cattle with Mycoplasma mycoides by nasal instillation. J Comp Pathol. 1968 Jul;78(3):383–386. [PubMed]
  • Stone SS. Comparison of bovine serum and colostral antibody: effect of colostral antibody on vaccination of calves for contagious bovine pleuropneumonia. Immunology. 1970 Mar;18(3):369–377. [PMC free article] [PubMed]
  • Stone SS, Bygrave AC. Contagious bovine pleuropneumonia: comparison of serological tests and post-mortem observations in cattle with resolving lung lesions. Bull Epizoot Dis Afr. 1969 Mar;17(1):11–19. [PubMed]
  • Stone SS, Masiga WN, Read WC. Mycoplasma mycoides transplacental transfer in cattle. Res Vet Sci. 1969 Jul;10(4):368–372. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...