• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Jul 1974; 10(1): 197–203.
PMCID: PMC414977

Diminished Virulence of Glucan Synthesis-Defective Mutants of Streptococcus mutans

Abstract

The virulence of cell surface-associated, glucan synthesis-defective mutants of Streptococcus mutans strain 6715-13 was studied. Representatives from three groups of such mutants were tested for their pathogenicity in conventionalized, specific pathogen-free rats and gnotobiotic rats. The mutants differ from the wild-type strain in that each failed to form plaque on the smooth surfaces of the teeth and to cause smooth surface caries. Although the ability to form cell surface-associated glucans was not a strict requirement for the expression of virulence in the sulci of the teeth, it augmented virulence at such sites. However, the ability to form cell surface-associated glucans and to adhere to the teeth was clearly not the sole determinant of virulence.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Berman KS, Gibbons RJ. Iodophilic polysaccharide synthesis by human and rodent oral bacteria. Arch Oral Biol. 1966 May;11(5):533–542. [PubMed]
  • BOWEN WH. A BACTERIOLOGICAL STUDY OF EXPERIMENTAL DENTAL CARIES IN MONKEYS. Int Dent J. 1965 Mar;15:12–53. [PubMed]
  • Bowen WH. The induction of rampant dental caries in monkeys (Macaca irus). Caries Res. 1969;3(3):227–237. [PubMed]
  • Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed]
  • Bratthall D. Immunofluorescent identification of Streptococcus mutans. Odontol Revy. 1972;23(2):181–196. [PubMed]
  • Brown AT, Patterson CE. Heterogeneity of Streptococcus mutans strains based on their mannitol-1-phosphate dehydrogenases: criterion for rapid classification. Infect Immun. 1972 Sep;6(3):422–424. [PMC free article] [PubMed]
  • Brown AT, Wittenberger CL. Fructose-1,6-diphosphate-dependent lactate dehydrogenase from a cariogenic streptococcus: purification and regulatory properties. J Bacteriol. 1972 May;110(2):604–615. [PMC free article] [PubMed]
  • Carlsson J. A levansucrase from Streptococcus mutans. Caries Res. 1970;4(2):97–113. [PubMed]
  • Charlton G, Fitzgerald RJ, Keyes PH. Determination of saliva and dental plaque pH in hamsters with glass micro-electrodes. Arch Oral Biol. 1971 Jun;16(6):649–654. [PubMed]
  • Charlton G, Fitzgerald DB, Keyes PH. Hydrogen ion activity in dental plaques of hamsters during metabolism of sucrose, glucose and fructose. Arch Oral Biol. 1971 Jun;16(6):655–661. [PubMed]
  • DaCosta T, Gibbons RJ. Hydrolysis of levan by human plaque streptococci. Arch Oral Biol. 1968 Jun;13(6):609–617. [PubMed]
  • Coykendall AL. Base composition of deoxyribonucleic acid isolated from cariogenic streptococci. Arch Oral Biol. 1970 Apr;15(4):365–368. [PubMed]
  • De Stoppelaar JD, Van Houte J, Backer Dirks O. The relationship between extracellular polysaccharide-producing streptococci and smooth surface caries in 13-year-old children. Caries Res. 1969;3(2):190–199. [PubMed]
  • de Stoppelaar JD, König KG, Plasschaert AJ, van der Hoeven JS. Decreased cariogenicity of a mutant of Streptococcus mutans. Arch Oral Biol. 1971 Aug;16(8):971–975. [PubMed]
  • Dunny GM, Hausner T, Clewell DB. Buoyant densities of DNA from various strains of Streptococcus mutans. Arch Oral Biol. 1972 Jun;17(6):1001–1003. [PubMed]
  • Edwardsson S. Characteristics of caries-inducing human streptococci resembling Streptococcus mutans. Arch Oral Biol. 1968 Jun;13(6):637–646. [PubMed]
  • Edwardsson S. The caries-inducing property of variants of Streptococcus mutans. Odontol Revy. 1970;21(2):153–157. [PubMed]
  • Fitzgerlad RJ. Dental caries research in gnotobiotic animals. Caries Res. 1968;2(2):139–146. [PubMed]
  • FITZGERALD RJ, JORDAN HV, STANLEY HR. Experimental caries and gingival pathologic changes in the gnotobiotic rat. J Dent Res. 1960 Sep-Oct;39:923–935. [PubMed]
  • FITZGERALD RJ, KEYES PH. Demonstration of the etiologic role of streptococci in experimental caries in the hamster. J Am Dent Assoc. 1960 Jul;61:9–19. [PubMed]
  • Fitzgerald RJ, Larson RH. Effect of invertase administration on dental caries in rats. Arch Oral Biol. 1971 Jun;16(6):671–673. [PubMed]
  • Fitzgerald RJ, Spinell DM, Stoudt TH. Enzymatic removal of artificial plaques. Arch Oral Biol. 1968 Jan;13(1):125–128. [PubMed]
  • Freedman ML, Tanzer JM. Dissociation of plaque formation from glucan-induced agglutination in mutants of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):189–196. [PMC free article] [PubMed]
  • Gibbons RJ, Berman KS, Knoettner P, Kapsimalis B. Dental caries and alveolar bone loss in gnotobiotic rats infected with capsule forming streptococci of human origin. Arch Oral Biol. 1966 Jun;11(6):549–560. [PubMed]
  • Gibbons RJ, Fitzgerald RJ. Dextran-induced agglutination of Streptococcus mutans, and its potential role in the formation of microbial dental plaques. J Bacteriol. 1969 May;98(2):341–346. [PMC free article] [PubMed]
  • Gibbons RJ, Nygaard M. Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Arch Oral Biol. 1968 Oct;13(10):1249–1262. [PubMed]
  • Guggenheim B, König KG, Herzog E, Mühlemann HR. The cariogenicity of different dietary carbohydrates tested on rats in relative gnotobiosis with a Streptococcus producing extracellular polysaccharide. Helv Odontol Acta. 1966 Oct;10(2):101–113. [PubMed]
  • Guggenheim B, Regolati B, Mühlemann HR. Caries and plaque inhibition by mutanase in rats. Caries Res. 1972;6(4):289–297. [PubMed]
  • Guggenheim B, Schroeder HE. Biochemical and morphological aspects of extracellular polysaccharides produced by cariogenic streptococci. Helv Odontol Acta. 1967 Oct;11(2):131–152. [PubMed]
  • GUSTAFSSON BE. Lightweight stainless steel systems for rearing germfree animals. Ann N Y Acad Sci. 1959 May 8;78:17–28. [PubMed]
  • Ikeda T, Sandham HJ. Prevalence of Streptococcus mutans on various tooth surfaces in Negro children. Arch Oral Biol. 1971 Oct;16(10):1237–1240. [PubMed]
  • Jordan HV, Krasse B, Möller A. A method of sampling human dental plaque for certain "caries-inducing" streptococci. Arch Oral Biol. 1968 Aug;13(8):919–927. [PubMed]
  • KEYES PH. Dental caries in the molar teeth of rats. II. A method for diagnosing and scoring several types of lesions simultaneously. J Dent Res. 1958 Nov-Dec;37(6):1088–1099. [PubMed]
  • KEYES PH, JORDAN HV. PERIODONTAL LESIONS IN THE SYRIAN HAMSTER. III. FINDINGS RELATED TO AN INFECTIOUS AND TRANSMISSIBLE COMPONENT. Arch Oral Biol. 1964 Jul-Aug;9:377–400. [PubMed]
  • KONIG KG. Dental morphology in relation to caries resistance with special reference to fissures as susceptible areas. J Dent Res. 1963 Jan-Feb;2:461–476. [PubMed]
  • König KG, Guggenheim B, Mühlemann HR. Modifications of the oral bacterial flora and their influence on dental caries in the rat. II. Inoculation of a cariogenic streptococcus and its effect in relation to varying time of depression of the indigenous flora. Helv Odontol Acta. 1965 Oct;9(2):130–134. [PubMed]
  • König KG, Larson RH, Guggenheim B. A strain-specific eating pattern as a factor limiting the transmissibility of caries activity in rats. Arch Oral Biol. 1969 Jan;14(1):91–103. [PubMed]
  • Krasse B. Human streptococci and experimental caries in hamsters. Arch Oral Biol. 1966 Apr;11(4):429–436. [PubMed]
  • Krasse B, Carlsson J. Various types of streptococci and experimental caries in hamsters. Arch Oral Biol. 1970 Jan;15(1):25–32. [PubMed]
  • Krasse B, Edwardsson S. The proportional distribution of caries-inducing streptococci in various parts of the oral cavity of hamsters. Arch Oral Biol. 1966 Nov;11(11):1137–1142. [PubMed]
  • Krasse B, Jordan HV, Edwardsson S, Svensson I, Trell L. The occurrence of certain "caries-inducing" streptococci in human dental plaque material with special reference to frequency and activity of caries. Arch Oral Biol. 1968 Aug;13(8):911–918. [PubMed]
  • Littleton NW, Kakehashi S, Fitzgerald RJ. Recovery of specific "caries-inducing" streptococci from carious lesions in the teeth of children. Arch Oral Biol. 1970 May;15(5):461–463. [PubMed]
  • Parker RB, Creamer HR. Contribution of plaque polysaccharides to growth of cariogenic microorganisms. Arch Oral Biol. 1971 Aug;16(8):855–862. [PubMed]
  • SCHAEDLER RW, DUBOS R, COSTELLO R. THE DEVELOPMENT OF THE BACTERIAL FLORA IN THE GASTROINTESTINAL TRACT OF MICE. J Exp Med. 1965 Jul 1;122:59–66. [PMC free article] [PubMed]
  • Tanzer JM, Hageage GJ. Polyphosphate inhibition of growth of plaques formed by streptococci and diphtheroids implicated in oral disease. Infect Immun. 1970 Jun;1(6):604–606. [PMC free article] [PubMed]
  • Tanzer JM, Hageage GJ, Jr, Larson RH. Variable experiences in immunization of rats against Streptococcus mutans-associated dental caries. Arch Oral Biol. 1973 Nov;18(11):1425–1439. [PubMed]
  • TREXLER PC. The use of plastics in the design of isolator systems. Ann N Y Acad Sci. 1959 May 8;78:29–36. [PubMed]
  • Van Houte J, Gibbons RJ, Banghart SB. Adherence as a determinant of the presence of Streptococcus salivarius and Streptococcus sanguis on the human tooth surface. Arch Oral Biol. 1970 Nov;15(11):1025–1034. [PubMed]
  • Van Houte J, Gibbons RJ, Pulkkinen AJ. Adherence as an ecological determinant for streptococci in the human mouth. Arch Oral Biol. 1971 Oct;16(10):1131–1141. [PubMed]
  • ZINNER DD, JABLON JM, ARAN AP, SASLAW MS. EXPERIMENTAL CARIES INDUCED IN ANIMALS BY STREPTOCOCCI OF HUMAN ORIGIN. Proc Soc Exp Biol Med. 1965 Mar;118:766–770. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...