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Proc Natl Acad Sci U S A. Jul 18, 1995; 92(15): 7070–7074.
PMCID: PMC41473

Dynamics of maize endosperm development and DNA endoreduplication.

Abstract

Endosperm development in Zea mays is characterized by a period of intense mitotic activity followed by a period in which mitosis is essentially eliminated and the cell cycle becomes one of alternating S and G phases, leading to endoreduplication of the nuclear DNA. The endosperm represents a significant contribution to the grain yield of maize; thus, methods that facilitate the study of cellular kinetics may be useful in discerning cellular and molecular components of grain yield. Two mathematical models have been developed to describe the kinetics of endosperm growth. The first describes the kinetics of mitosis during endosperm development; the second describes the kinetics of DNA endoreduplication during endosperm development. The mitotic model is a modification of standard growth curves. The endoreduplication model is composed of six differential equations that represent the progression of nuclei from one DNA content to another during the endoreduplication process. Total nuclei number per endosperm and the number of 3C, 6C, 12C, 24C, 48C, and 96C nuclei per endosperm (C is the haploid DNA content per nucleus) for inbred W64A from 8 to 18 days after pollination were determined by flow cytometry. The results indicate that the change in number of nuclei expressed as a function of the number of days after pollination is the same from one yearly crop to another. These data were used in the model to determine the endosperm growth rate, the maximum nuclei number per endosperm, and transition rates from one C value to the next higher C value. The kinetics of endosperm development are reasonably well represented by the models. Thus, the models provide a means to quantify the complex pattern of endosperm development.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Kowles RV, Phillips RL. DNA amplification patterns in maize endosperm nuclei during kernel development. Proc Natl Acad Sci U S A. 1985 Oct;82(20):7010–7014. [PMC free article] [PubMed]
  • Azaizeh HA, Pettit RE, Sarr BA, Phillips TD. Effect of peanut tannin extracts on growth of Aspergillus parasiticus and aflatoxin production. Mycopathologia. 1990 Jun;110(3):125–132. [PubMed]

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