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Proc Natl Acad Sci U S A. Oct 1979; 76(10): 5138–5142.
PMCID: PMC413095

A study of the adipose conversion of suspended 3T3 cells by using glycerophosphate dehydrogenase as differentiation marker


The adipose conversion of 3T3 cells has been examined in stabilized suspension cultures. In 3T3-F442A cells, glycerophosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC, a key enzyme in triglyceride synthesis, increases in specific activity by more than 5000-fold and can be used as a sensitive and precise measure of the conversion. The conversion depends on an adipogenic factor present in the serum, and this factor can be assayed by the cellular enzyme response. If the cells are growing at the time they receive the adipogenic factor, the enzyme response does not become detectable until after 3 days, during which the cells first enter a resting state. If the cells are resting at the time they receive the adipogenic factor, the enzyme activity begins to increase in 24 hr or less. Only resting cells seem susceptible to the reprogramming of their differentiated state necessary for the adipose conversion. Once the conversion begins, the increase in enzyme activity is exponential over at least 2 orders of magnitude. When cells in a resting state begin the adipose conversion, their biosynthetic processes are accelerated: the rate of protein synthesis increases, they accumulate cell protein, and they may replicate their DNA and divide. The cell multiplication is not essential for adipose conversion but is a form of clonal selection that increases the proportion of adipose cells relative to nonadipose cells.

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Selected References

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  • Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell. 1974 Oct;3(2):127–133. [PubMed]
  • Mackall JC, Student AK, Polakis SE, Lane MD. Induction of lipogenesis during differentiation in a "preadipocyte" cell line. J Biol Chem. 1976 Oct 25;251(20):6462–6464. [PubMed]
  • Kuri-Harcuch W, Green H. Increasing activity of enzymes on pathway of triacylglycerol synthesis during adipose conversion of 3T3 cells. J Biol Chem. 1977 Mar 25;252(6):2158–2160. [PubMed]
  • Eckel RH, Fujimoto WY, Brunzell JD. Development of lipoprotein lipase in cultured 3T3-L1 cells. Biochem Biophys Res Commun. 1977 Sep 9;78(1):288–293. [PubMed]
  • Grimaldi P, Négrel R, Ailhaud G. Induction of the triglyceride pathway enzymes and of lipolytic enzymes during differentiation in a 'preadipocyte' cell line. Eur J Biochem. 1978 Mar 15;84(2):369–376. [PubMed]
  • Wise LS, Green H. Studies of lipoprotein lipase during the adipose conversion of 3T3 cells. Cell. 1978 Feb;13(2):233–242. [PubMed]
  • Coleman RA, Reed BC, Mackall JC, Student AK, Lane MD, Bell RM. Selective changes in microsomal enzymes of triacylglycerol phosphatidylcholine, and phosphatidylethanolamine biosynthesis during differentiation of 3T3-L1 preadipocytes. J Biol Chem. 1978 Oct 25;253(20):7256–7261. [PubMed]
  • Reed BC, Kaufmann SH, Mackall JC, Student AK, Lane MD. Alterations in insulin binding accompanying differentiation of 3T3-L1 preadipocytes. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4876–4880. [PMC free article] [PubMed]
  • Rubin CS, Lai E, Rosen OM. Acquisition of increased hormone sensitivity during in vitro adipocyte development. J Biol Chem. 1977 May 25;252(10):3554–3557. [PubMed]
  • Karlsson FA, Van Obberghen E, Grunfeld C, Kahn CR. Desensitization of the insulin receptor at an early postreceptor step by prolonged exposure to antireceptor antibody. Proc Natl Acad Sci U S A. 1979 Feb;76(2):809–813. [PMC free article] [PubMed]
  • Kuri-Harcuch W, Wise LS, Green H. Interruption of the adipose conversion of 3T3 cells by biotin deficiency: differentiation without triglyceride accumulation. Cell. 1978 May;14(1):53–59. [PubMed]
  • Wise LS, Green H. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. J Biol Chem. 1979 Jan 25;254(2):273–275. [PubMed]
  • Kuri-Harcuch W, Green H. Adipose conversion of 3T3 cells depends on a serum factor. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6107–6109. [PMC free article] [PubMed]
  • Green H, Kehinde O. Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells. Cell. 1976 Jan;7(1):105–113. [PubMed]
  • Green H. Terminal differentiation of cultured human epidermal cells. Cell. 1977 Jun;11(2):405–416. [PubMed]
  • Kozak LP, Jensen JT. Genetic and developmental control of multiple forms of L-glycerol 3-phosphate dehydrogenase. J Biol Chem. 1974 Dec 25;249(24):7775–7781. [PubMed]
  • Green H, Kehinde O. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell. 1975 May;5(1):19–27. [PubMed]
  • Morrissey JH, Green H. Differentiation-related death of an established keratinocyte line in suspension culture. J Cell Physiol. 1978 Dec;97(3 Pt 2 Suppl 1):469–476. [PubMed]
  • Benecke BJ, Ben-Ze'ev A, Penman S. The control of mRNA production, translation and turnover in suspended and reattached anchorage-dependent fibroblasts. Cell. 1978 Aug;14(4):931–939. [PubMed]
  • Pilgrim C. DNA synthesis and differentiation in developing white adipose tissue. Dev Biol. 1971 Sep;26(1):69–76. [PubMed]

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