Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Mar 1978; 75(3): 1314–1318.
PMCID: PMC411461

Regulation of acetylcholine release from neuroblastoma x glioma hybrid cells.


Neuroblastoma x glioma NG108-15 hybrid cells exposed to N6, O2'-dibutyryladenosine 3':5'-cyclic monophosphate for several days release [3H]acetylcholine in response to serotonin, prostaglandin F2alpha, KCl, or veratridine. NG108-15 cells grown in the absence of dibutyrul cyclic AMP do not respond to an excitatory stimulus by releasing [3H]acetylcholine but can be shifted to a responsive state by treatment with dibutyryl cyclic AMP. Thus, the reactions that are required for acetylcholine release can be regulated in NG108-15 cells, thereby regulating the ability of cells to form synapses and the efficiency of synaptic communication.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Nelson P, Christian C, Nirenberg M. Synapse formation between clonal neuroblastoma X glioma hybrid cells and striated muscle cells. Proc Natl Acad Sci U S A. 1976 Jan;73(1):123–127. [PMC free article] [PubMed]
  • Puro DG, Nirenberg M. On the specificity of synapse formation. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3544–3548. [PMC free article] [PubMed]
  • Christian CN, Nelson PG, Peacock J, Nirenberg M. Synapse formation between two clonal cell lines. Science. 1977 May 27;196(4293):995–998. [PubMed]
  • Daniels MP, Hamprecht B. The ultrastructure of neuroblastoma glioma somatic cell hybrids. Expression of neuronal characteristics stimulated by dibutyryl adenosine 3',5' cyclic monophosphate. J Cell Biol. 1974 Nov;63(2 Pt 1):691–699. [PMC free article] [PubMed]
  • Traber J, Fischer K, Latzin S, Hamprecht B. Morphine antagonises action of prostaglandin in neuroblastoma and neuroblastoma times glioma hybrid cells. Nature. 1975 Jan 10;253(5487):120–122. [PubMed]
  • Sharma SK, Klee WA, Nirenberg M. Dual regulation of adenylate cyclase accounts for narcotic dependence and tolerance. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3092–3096. [PMC free article] [PubMed]
  • Traber J, Fischer K, Buchen C, Hamprecht B. Muscarinic response to acetylcholine in neuroblastoma times glioma hybrid cells. Nature. 1975 Jun 12;255(5509):558–560. [PubMed]
  • Traber J, Reiser G, Fischer K, Hamprecht B. Measurements of adenosine 3':5'-cyclic monophosphate and membrane potential in neuroblastoma times glioma hybrid cells: opiates and adrenergic agonists cause effects opposite to those of prostaglandin E1. FEBS Lett. 1975 Apr 1;52(2):327–332. [PubMed]
  • Traber J, Fischer K, Latzin S, Hamprecht B. Morphine antagonizes the action of prostaglandin in neuroblastoma cells but not of prostaglandin and noradrenaline in glioma and glioma times fibroblast hybrid cells. FEBS Lett. 1974 Dec 15;49(2):260–263. [PubMed]
  • Myers PR, Livengood DR. Dopamine depolarising response in a vertebrate neuronal somatic cell hybrid. Nature. 1975 May 15;255(5505):235–236. [PubMed]
  • Toru M, Aprison MH. Brain acetylcholine studies: a new extraction procedure. J Neurochem. 1966 Dec;13(12):1533–1544. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Potter LT, Murphy W. Electrophoresis of acetylcholine, choline and related compounds. Biochem Pharmacol. 1967 Jul 7;16(7):1386–1388. [PubMed]
  • Goldberg AM, McCaman RE. The determination of picomole amounts of acetylcholine in mammalian brain. J Neurochem. 1973 Jan;20(1):1–8. [PubMed]
  • Yavin E. Regulation of phospholipid metabolism in differentiating cells from rat brain cerebral hemispheres in culture. Patterns of acetylcholine phosphocholine, and choline phosphoglycerides labeling from (methyl-14C)choline. J Biol Chem. 1976 Mar 10;251(5):1392–1397. [PubMed]
  • Yamamura HI, Snyder SH. High affinity transport of choline into synaptosomes of rat brain. J Neurochem. 1973 Dec;21(6):1355–1374. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...