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Proc Natl Acad Sci U S A. 1978 Jan; 75(1): 185–189.
PMCID: PMC411210

Specificity of the juvenile hormone binding protein: The geometrical isomers of juvenile hormone I


The binding of the geometrical isomers (≥99% pure) of juvenile hormone I to the hemolymph juvenile hormone binding protein of Manduca sexta (Lepidoptera, Sphingidae) was analyzed. A technique is described for isomer separation by micropreparative high-resolution liquid chromatography. Analysis of competition was performed by using a “batch adsorption” hydroxylapatite binding assay. Competition studies indicate that the naturally occurring isomer, 2E,6E,10cis, is bound with the highest affinity. Optimal binding appears to depend most heavily upon the configuration of the 2,3 double bond. Juvenile hormone binding protein shows a higher affinity for the 2E than for the 2Z configuration. The 6,7 double bond is of less importance in determining binding activity, and isomerism about the epoxide appears least important in conferring binding activity. The binding site may be a groove along the surface of the binding protein interacting with the side chains of juvenile hormone, including the ester methyl group. The grouping of the side chains and the ester methyl group thus constitutes a distinct hydrophobic face, and the hydrophobic interactions are essential in maintenance of the bound ligand.

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  • Kramer KJ, Sanburg LL, Kézdy FJ, Law JH. The Juvenile Hormone Binding Protein in the Hemolymph of Manduca sexta Johannson (Lepidoptera: Sphingidae). Proc Natl Acad Sci U S A. 1974 Feb;71(2):493–497. [PMC free article] [PubMed]
  • Röller H, Dahm KH. The chemistry and biology of juvenile hormone. Recent Prog Horm Res. 1968;24:651–680. [PubMed]
  • Low RB, Wool IG, Martin TE. Skeletal muscle ribosomal proteins: general characteristics and effect of diabetes. Biochim Biophys Acta. 1969 Nov 11;194(1):190–202. [PubMed]
  • Trautmann KH, Schuler A, Suchý M, Wipf HK. Eine Methode zur qualitativen und quantitativen Bestimmung von drei Juvenilhormonen von Insekten. Nachweis von 10.11-Epoxy-3.7.11-trimethyl-2-trans-6-trans-dodecadiensäurementylester in Mellontha melolontha. Z Naturforsch C. 1974 Mar-Apr;29(3):161–168. [PubMed]
  • Pavlik EJ, Coulson PB. Hydroxylapatite "batch" assay for estrogen receptors: increased sensitivity over present receptor assays. J Steroid Biochem. 1976 May;7(5):357–368. [PubMed]
  • Peterson RC, Reich MF, Dunn PE, Law JH, Katzenellnbogen JA. Binding specificity of the juvenile hormone carrier protein from the hemolymph of the tobacco hornworm Manduca sexta Johannson (Lepidoptera: Sphingidae). Biochemistry. 1977 May 17;16(10):2305–2311. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


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