• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Sep 12, 1995; 92(19): 8675–8679.
PMCID: PMC41029

A chloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis.

Abstract

Plant lipoxygenases are thought to be involved in the biosynthesis of lipid-derived signaling molecules. The potential involvement of a specific Arabidopsis thaliana lipoxygenase isozyme, LOX2, in the biosynthesis of the plant growth regulators jasmonic acid (JA) and abscisic acid was investigated. Our characterization of LOX2 indicates that the protein is targeted to chloroplasts. The physiological role of this chloroplast lipoxygenase was analyzed in transgenic plants where cosuppression reduced LOX2 accumulation. The reduction in LOX2 levels caused no obvious changes in plant growth or in the accumulation of abscisic acid. However, the wound-induced accumulation of JA observed in control plants was absent in leaves of transgenic plants that lacked LOX2. Thus, LOX2 is required for the wound-induced synthesis of the plant growth regulator JA in leaves. We also examined the expression of a wound- and JA-inducible Arabidopsis gene, vsp, in transgenic and control plants. Leaves of transgenic plants lacking LOX2 accumulated less vsp mRNA than did control leaves in response to wounding. This result suggests that wound-induced JA (or some other LOX2-requiring component of the wound response pathway) is involved in the wound-induced regulation of this gene.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Samuelsson B, Dahlén SE, Lindgren JA, Rouzer CA, Serhan CN. Leukotrienes and lipoxins: structures, biosynthesis, and biological effects. Science. 1987 Sep 4;237(4819):1171–1176. [PubMed]
  • Bell E, Mullet JE. Lipoxygenase gene expression is modulated in plants by water deficit, wounding, and methyl jasmonate. Mol Gen Genet. 1991 Dec;230(3):456–462. [PubMed]
  • Bell E, Mullet JE. Characterization of an Arabidopsis lipoxygenase gene responsive to methyl jasmonate and wounding. Plant Physiol. 1993 Dec;103(4):1133–1137. [PMC free article] [PubMed]
  • Koch E, Meier BM, Eiben HG, Slusarenko A. A Lipoxygenase from Leaves of Tomato (Lycopersicon esculentum Mill.) Is Induced in Response to Plant Pathogenic Pseudomonads. Plant Physiol. 1992 Jun;99(2):571–576. [PMC free article] [PubMed]
  • Ohta H, Shida K, Peng YL, Furusawa I, Shishiyama J, Aibara S, Morita Y. A Lipoxygenase Pathway Is Activated in Rice after Infection with the Rice Blast Fungus Magnaporthe grisea. Plant Physiol. 1991 Sep;97(1):94–98. [PMC free article] [PubMed]
  • Creelman RA, Tierney ML, Mullet JE. Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4938–4941. [PMC free article] [PubMed]
  • Mueller MJ, Brodschelm W, Spannagl E, Zenk MH. Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid. Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7490–7494. [PMC free article] [PubMed]
  • Creelman RA, Bell E, Mullet JE. Involvement of a lipoxygenase-like enzyme in abscisic Acid biosynthesis. Plant Physiol. 1992 Jul;99(3):1258–1260. [PMC free article] [PubMed]
  • Melan MA, Dong X, Endara ME, Davis KR, Ausubel FM, Peterman TK. An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. Plant Physiol. 1993 Feb;101(2):441–450. [PMC free article] [PubMed]
  • Baumgartner BJ, Rapp JC, Mullet JE. Plastid transcription activity and DNA copy number increase early in barley chloroplast development. Plant Physiol. 1989 Mar;89(3):1011–1018. [PMC free article] [PubMed]
  • Carrington JC, Freed DD. Cap-independent enhancement of translation by a plant potyvirus 5' nontranslated region. J Virol. 1990 Apr;64(4):1590–1597. [PMC free article] [PubMed]
  • Valvekens D, Montagu MV, Van Lijsebettens M. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536–5540. [PMC free article] [PubMed]
  • Creelman RA, Mullet JE. Jasmonic acid distribution and action in plants: regulation during development and response to biotic and abiotic stress. Proc Natl Acad Sci U S A. 1995 May 9;92(10):4114–4119. [PMC free article] [PubMed]
  • Creelman RA, Zeevaart JA. Incorporation of oxygen into abscisic Acid and phaseic Acid from molecular oxygen. Plant Physiol. 1984 May;75(1):166–169. [PMC free article] [PubMed]
  • Flavell RB. Inactivation of gene expression in plants as a consequence of specific sequence duplication. Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3490–3496. [PMC free article] [PubMed]
  • Rock CD, Zeevaart JA. The aba mutant of Arabidopsis thaliana is impaired in epoxy-carotenoid biosynthesis. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7496–7499. [PMC free article] [PubMed]
  • Vick BA, Zimmerman DC. The biosynthesis of jasmonic acid: a physiological role for plant lipoxygenase. Biochem Biophys Res Commun. 1983 Mar 16;111(2):470–477. [PubMed]
  • Berger S, Bell E, Sadka A, Mullet JE. Arabidopsis thaliana Atvsp is homologous to soybean VspA and VspB, genes encoding vegetative storage protein acid phosphatases, and is regulated similarly by methyl jasmonate, wounding, sugars, light and phosphate. Plant Mol Biol. 1995 Mar;27(5):933–942. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...