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Proc Natl Acad Sci U S A. Oct 24, 1995; 92(22): 10114–10118.
PMCID: PMC40746

A Drosophila seminal fluid protein, Acp26Aa, stimulates egg laying in females for 1 day after mating.

Abstract

Mating triggers behavioral and physiological changes in the Drosophila melanogaster female, including an elevation of egg laying. Seminal fluid molecules from the male accessory gland are responsible for initial behavioral changes, but persistence of these changes requires stored sperm. Using genetic analysis, we have identified a seminal fluid protein that is responsible for an initial elevation of egg laying. This molecule, Acp26Aa, has structural features of a prohormone and contains a region with amino acid similarity to the egg-laying hormone of Aplysia. Acp26Aa is transferred to the female during mating, where it undergoes processing. Here we report the generation and analysis of mutants, including a null, in Acp26Aa. Females mated to male flies that lack Acp26Aa lay fewer eggs than do mates of normal males. This effect is apparent only on the first day after mating. The null mutation has no other detectable physiological or behavioral effects on the male or the mated female.

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Selected References

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  • Chapman T, Liddle LF, Kalb JM, Wolfner MF, Partridge L. Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products. Nature. 1995 Jan 19;373(6511):241–244. [PubMed]
  • Monsma SA, Wolfner MF. Structure and expression of a Drosophila male accessory gland gene whose product resembles a peptide pheromone precursor. Genes Dev. 1988 Sep;2(9):1063–1073. [PubMed]
  • Scheller RH, Jackson JF, McAllister LB, Rothman BS, Mayeri E, Axel R. A single gene encodes multiple neuropeptides mediating a stereotyped behavior. Cell. 1983 Jan;32(1):7–22. [PubMed]
  • Kalb JM, DiBenedetto AJ, Wolfner MF. Probing the function of Drosophila melanogaster accessory glands by directed cell ablation. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8093–8097. [PMC free article] [PubMed]
  • Manning A. The control of sexual receptivity in female Drosophila. Anim Behav. 1967 Apr-Jul;15(2):239–250. [PubMed]
  • GARCIA-BELLIDO A. DAS SEKRET DER PARAGONIEN ALS STIMULUS DER FEKUNDITAET BEI WEIBCHEN VON DROSOPHILA MELANOGASTER. Z Naturforsch B. 1964 Jun;19:491–495. [PubMed]
  • Merle J. Fonctionnement ovarien et réceptivité sexuelle de Drosophila melanogaster après implantation de fragments de l'appareil génital mâle. J Insect Physiol. 1968 Aug;14(8):1159–1168. [PubMed]
  • Chen PS, Stumm-Zollinger E, Aigaki T, Balmer J, Bienz M, Böhlen P. A male accessory gland peptide that regulates reproductive behavior of female D. melanogaster. Cell. 1988 Jul 29;54(3):291–298. [PubMed]
  • Monsma SA, Harada HA, Wolfner MF. Synthesis of two Drosophila male accessory gland proteins and their fate after transfer to the female during mating. Dev Biol. 1990 Dec;142(2):465–475. [PubMed]
  • Clark AG, Aguadé M, Prout T, Harshman LG, Langley CH. Variation in sperm displacement and its association with accessory gland protein loci in Drosophila melanogaster. Genetics. 1995 Jan;139(1):189–201. [PMC free article] [PubMed]
  • Van Vactor D, Jr, Krantz DE, Reinke R, Zipursky SL. Analysis of mutants in chaoptin, a photoreceptor cell-specific glycoprotein in Drosophila, reveals its role in cellular morphogenesis. Cell. 1988 Jan 29;52(2):281–290. [PubMed]
  • DiBenedetto AJ, Lakich DM, Kruger WD, Belote JM, Baker BS, Wolfner MF. Sequences expressed sex-specifically in Drosophila melanogaster adults. Dev Biol. 1987 Jan;119(1):242–251. [PubMed]
  • Bialojan S, Falkenburg D, Renkawitz-Pohl R. Characterization and developmental expression of beta tubulin genes in Drosophila melanogaster. EMBO J. 1984 Nov;3(11):2543–2548. [PMC free article] [PubMed]
  • Aguadé M, Miyashita N, Langley CH. Polymorphism and divergence in the Mst26A male accessory gland gene region in Drosophila. Genetics. 1992 Nov;132(3):755–770. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem. 1983 Jun 1;133(1):17–21. [PubMed]
  • Sachs AB. Messenger RNA degradation in eukaryotes. Cell. 1993 Aug 13;74(3):413–421. [PubMed]
  • Szabad J, Fajszi C. Control of female reproduction in Drosophila: genetic dissection using gynandromorphs. Genetics. 1982 Jan;100(1):61–78. [PMC free article] [PubMed]
  • Tompkins L, Siegel RW, Gailey DA, Hall JC. Conditioned courtship in Drosophila and its mediation by association of chemical cues. Behav Genet. 1983 Nov;13(6):565–578. [PubMed]
  • Boulétreau-Merle J. Stimulation de l'ovogenèse par la copulation chez les femelles de Drosophila melanogaster privées de leur complexe endocrine rétrocérébral. J Insect Physiol. 1974 Oct;20(10):2035–2041. [PubMed]
  • Boulétreau-Merle J. Destruction de la pars intercerebralis chez Drosophila melanogaster: effet sur la fécondité et sur sa stimulation par l'accouplement. J Insect Physiol. 1976;22(7):933–940. [PubMed]
  • Aigaki T, Fleischmann I, Chen PS, Kubli E. Ectopic expression of sex peptide alters reproductive behavior of female D. melanogaster. Neuron. 1991 Oct;7(4):557–563. [PubMed]
  • Kaldany RR, Nambu JR, Scheller RH. Neuropeptides in identified Aplysia neurons. Annu Rev Neurosci. 1985;8:431–455. [PubMed]
  • Schmidt T, Choffat Y, Schneider M, Hunziker P, Fuyama Y, Kubli E. Drosophila suzukii contains a peptide homologous to the Drosophila melanogaster sex-peptide and functional in both species. Insect Biochem Mol Biol. 1993 Jul;23(5):571–579. [PubMed]

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