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EMBO J. Dec 1, 1989; 8(12): 3553–3562.
PMCID: PMC402034

Molecular analysis of the asense gene, a member of the achaete-scute complex of Drosophila melanogaster, and its novel role in optic lobe development.


The achaete-scute complex (AS-C) comprises five genetic regions: achaete, scute (sc) alpha, lethal of sc, sc beta and sc gamma. Each region promotes the determination and positional specification of different, but partially overlapping, subsets of neural elements of Drosophila. In this work, we report a molecular characterization of the sc gamma region. It comprises 22 kb of DNA and contains two transcription units, only one of which, named asense (ase), seems involved in neurogenesis. ase encodes a protein that shares with other three AS-C proteins a domain containing a helix--loop--helix motif characteristic of a group of DNA-binding proteins. In the embryo, ase is expressed in neural precursor cells, a pattern consistent with the known requirement of sc gamma for the development of the larval nervous system. In late third-instar larvae, the gene is expressed in developing structures of the central nervous system (CNS), namely the anlagen of the optic lobes and in many cells, including neuroblasts, of the central brain and ventral ganglia. Its removal leads to anatomical defects in the adult optic lobes. This is the first demonstration of a role for the AS-C in the development of the adult CNS.

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  • Alonso MC, Cabrera CV. The achaete-scute gene complex of Drosophila melanogaster comprises four homologous genes. EMBO J. 1988 Aug;7(8):2585–2591. [PMC free article] [PubMed]
  • Balcells L, Modolell J, Ruiz-Gómez M. A unitary basis for different Hairy-wing mutations of Drosophila melanogaster. EMBO J. 1988 Dec 1;7(12):3899–3906. [PMC free article] [PubMed]
  • Beamonte D, Modolell J. Search for Drosophila genes encoding a conserved domain present in the achaete-scute complex and myc proteins. Mol Gen Genet. 1989 Jan;215(2):281–285. [PubMed]
  • Bensi G, Raugei G, Klefenz H, Cortese R. Structure and expression of the human haptoglobin locus. EMBO J. 1985 Jan;4(1):119–126. [PMC free article] [PubMed]
  • Birnstiel ML, Busslinger M, Strub K. Transcription termination and 3' processing: the end is in site! Cell. 1985 Jun;41(2):349–359. [PubMed]
  • Braun T, Buschhausen-Denker G, Bober E, Tannich E, Arnold HH. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts. EMBO J. 1989 Mar;8(3):701–709. [PMC free article] [PubMed]
  • Cabrera CV, Martinez-Arias A, Bate M. The expression of three members of the achaete-scute gene complex correlates with neuroblast segregation in Drosophila. Cell. 1987 Jul 31;50(3):425–433. [PubMed]
  • Campuzano S, Carramolino L, Cabrera CV, Ruíz-Gómez M, Villares R, Boronat A, Modolell J. Molecular genetics of the achaete-scute gene complex of D. melanogaster. Cell. 1985 Feb;40(2):327–338. [PubMed]
  • Campuzano S, Balcells L, Villares R, Carramolino L, García-Alonso L, Modolell J. Excess function hairy-wing mutations caused by gypsy and copia insertions within structural genes of the achaete-scute locus of Drosophila. Cell. 1986 Jan 31;44(2):303–312. [PubMed]
  • Caudy M, Vässin H, Brand M, Tuma R, Jan LY, Jan YN. daughterless, a Drosophila gene essential for both neurogenesis and sex determination, has sequence similarities to myc and the achaete-scute complex. Cell. 1988 Dec 23;55(6):1061–1067. [PubMed]
  • Cavener DR. Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates. Nucleic Acids Res. 1987 Feb 25;15(4):1353–1361. [PMC free article] [PubMed]
  • Chia W, Howes G, Martin M, Meng YB, Moses K, Tsubota S. Molecular analysis of the yellow locus of Drosophila. EMBO J. 1986 Dec 20;5(13):3597–3605. [PMC free article] [PubMed]
  • Davis RL, Weintraub H, Lassar AB. Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell. 1987 Dec 24;51(6):987–1000. [PubMed]
  • Dente L, Cesareni G, Cortese R. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 1983 Mar 25;11(6):1645–1655. [PMC free article] [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Doe CQ, Kuwada JY, Goodman CS. From epithelium to neuroblasts to neurons: the role of cell interactions and cell lineage during insect neurogenesis. Philos Trans R Soc Lond B Biol Sci. 1985 Dec 17;312(1153):67–81. [PubMed]
  • Doe CQ, Hiromi Y, Gehring WJ, Goodman CS. Expression and function of the segmentation gene fushi tarazu during Drosophila neurogenesis. Science. 1988 Jan 8;239(4836):170–175. [PubMed]
  • Doe CQ, Smouse D, Goodman CS. Control of neuronal fate by the Drosophila segmentation gene even-skipped. Nature. 1988 May 26;333(6171):376–378. [PubMed]
  • Fischbach KF, Heisenberg M. Structural brain mutant of Drosophila melanogaster with reduced cell number in the medulla cortex and with normal optomotor yaw response. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1105–1109. [PMC free article] [PubMed]
  • Franke WW, Krien S, Brown RM., Jr Simultaneous glutaraldehyde-osmium tetroxide fixation with postosmication. An improved fixation procedure for electron microscopy of plant and animal cells. Histochemie. 1969;19(2):162–164. [PubMed]
  • García-Bellido A. Genetic Analysis of the Achaete-Scute System of DROSOPHILA MELANOGASTER. Genetics. 1979 Mar;91(3):491–520. [PMC free article] [PubMed]
  • Ghysen A, Dambly-Chaudière C. From DNA to form: the achaete-scute complex. Genes Dev. 1988 May;2(5):495–501. [PubMed]
  • Goodman CS, Bastiani MJ, Doe CQ, du Lac S, Helfand SL, Kuwada JY, Thomas JB. Cell recognition during neuronal development. Science. 1984 Sep 21;225(4668):1271–1279. [PubMed]
  • Guarente L. UASs and enhancers: common mechanism of transcriptional activation in yeast and mammals. Cell. 1988 Feb 12;52(3):303–305. [PubMed]
  • Ingham PW. Genetic control of the spatial pattern of selector gene expression in Drosophila. Cold Spring Harb Symp Quant Biol. 1985;50:201–208. [PubMed]
  • Jiménez F, Campos-Ortega JA. Genes in subdivision 1B of the Drosophila melanogaster X-chromosome and their influence on neural development. J Neurogenet. 1987 Jun;4(4):179–200. [PubMed]
  • Jiménez F, Campos-Ortega JA. A region of the Drosophila genome necessary for CNS development. Nature. 1979 Nov 15;282(5736):310–312. [PubMed]
  • Klämbt C, Knust E, Tietze K, Campos-Ortega JA. Closely related transcripts encoded by the neurogenic gene complex enhancer of split of Drosophila melanogaster. EMBO J. 1989 Jan;8(1):203–210. [PMC free article] [PubMed]
  • Maniatis T, Hardison RC, Lacy E, Lauer J, O'Connell C, Quon D, Sim GK, Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. [PubMed]
  • Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. [PMC free article] [PubMed]
  • Meyerowitz EM, Kankel DR. A genetic analysis of visual system development in Drosophilia melanogaster. Dev Biol. 1978 Jan;62(1):112–142. [PubMed]
  • Modolell J, Bender W, Meselson M. Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1678–1682. [PMC free article] [PubMed]
  • Murre C, McCaw PS, Baltimore D. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Cell. 1989 Mar 10;56(5):777–783. [PubMed]
  • Rogers S, Wells R, Rechsteiner M. Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis. Science. 1986 Oct 17;234(4774):364–368. [PubMed]
  • Romani S, Campuzano S, Modolell J. The achaete-scute complex is expressed in neurogenic regions of Drosophila embryos. EMBO J. 1987 Jul;6(7):2085–2092. [PMC free article] [PubMed]
  • Romani S, Campuzano S, Macagno ER, Modolell J. Expression of achaete and scute genes in Drosophila imaginal discs and their function in sensory organ development. Genes Dev. 1989 Jul;3(7):997–1007. [PubMed]
  • Rubin GM. Development of the Drosophila retina: inductive events studied at single cell resolution. Cell. 1989 May 19;57(4):519–520. [PubMed]
  • Ruiz-Gómez M, Modolell J. Deletion analysis of the achaete-scute locus of Drosophila melanogaster. Genes Dev. 1987 Dec;1(10):1238–1246. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Staden R. Computer methods to locate signals in nucleic acid sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):505–519. [PMC free article] [PubMed]
  • Tabor S, Richardson CC. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767–4771. [PMC free article] [PubMed]
  • Thisse B, Stoetzel C, Gorostiza-Thisse C, Perrin-Schmitt F. Sequence of the twist gene and nuclear localization of its protein in endomesodermal cells of early Drosophila embryos. EMBO J. 1988 Jul;7(7):2175–2183. [PMC free article] [PubMed]
  • Truman JW, Bate M. Spatial and temporal patterns of neurogenesis in the central nervous system of Drosophila melanogaster. Dev Biol. 1988 Jan;125(1):145–157. [PubMed]
  • Villares R, Cabrera CV. The achaete-scute gene complex of D. melanogaster: conserved domains in a subset of genes required for neurogenesis and their homology to myc. Cell. 1987 Jul 31;50(3):415–424. [PubMed]
  • Wharton KA, Yedvobnick B, Finnerty VG, Artavanis-Tsakonas S. opa: a novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster. Cell. 1985 Jan;40(1):55–62. [PubMed]
  • White K, Kankel DR. Patterns of cell division and cell movement in the formation of the imaginal nervous system in Drosophila melanogaster. Dev Biol. 1978 Aug;65(2):296–321. [PubMed]
  • Wright WE, Sassoon DA, Lin VK. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell. 1989 Feb 24;56(4):607–617. [PubMed]

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