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Proc Natl Acad Sci U S A. 1996 Jan 23; 93(2): 759–764.

Integration of multiple repeats of geminiviral DNA into the nuclear genome of tobacco during evolution.


Integration of viral DNA into the host nuclear genome, although not unusual in bacterial and animal systems, has surprisingly not been reported for plants. We have discovered geminvirus-related DNA (GRD) sequences, in the form of distinct sets of multiple direct repeats comprising three related repeat classes, situated in a unique locus in the Nicotiana tabacum (tobacco) nuclear genome. The organization of these sequences is similar or identical in eight different tobacco cultivars we have examined. DNA sequence analysis reveals that each repeat has sequences most resembling those of the New World geminiviral DNA replication origin plus the adjacent AL1 gene, encoding the viral replication protein. We believe these GRD sequences originated quite recently in Nicotiana evolution through integration of geminiviral DNA by some combination of the processes of illegitimate recombination, amplification, deletions, and rearrangements. These events must have occurred in plant tissue that was subsequently able to contribute to meristematic tissue yielding gametes. GRD may have been retained in tobacco by selection or by random fixation in a small evolving population. Although we cannot detect transcription of these sequences, this does not exclude the possibility that they may originally have been expressed.

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Selected References

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  • Greene AE, Allison RF. Recombination between viral RNA and transgenic plant transcripts. Science. 1994 Mar 11;263(5152):1423–1425. [PubMed]
  • Xiong Y, Eickbush TH. Origin and evolution of retroelements based upon their reverse transcriptase sequences. EMBO J. 1990 Oct;9(10):3353–3362. [PMC free article] [PubMed]
  • Chu G, Vollrath D, Davis RW. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582–1585. [PubMed]
  • Lazarowitz SG, Wu LC, Rogers SG, Elmer JS. Sequence-specific interaction with the viral AL1 protein identifies a geminivirus DNA replication origin. Plant Cell. 1992 Jul;4(7):799–809. [PMC free article] [PubMed]
  • Fontes EP, Eagle PA, Sipe PS, Luckow VA, Hanley-Bowdoin L. Interaction between a geminivirus replication protein and origin DNA is essential for viral replication. J Biol Chem. 1994 Mar 18;269(11):8459–8465. [PubMed]
  • Day AG, Bejarano ER, Buck KW, Burrell M, Lichtenstein CP. Expression of an antisense viral gene in transgenic tobacco confers resistance to the DNA virus tomato golden mosaic virus. Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6721–6725. [PMC free article] [PubMed]
  • Koonin EV, Ilyina TV. Geminivirus replication proteins are related to prokaryotic plasmid rolling circle DNA replication initiator proteins. J Gen Virol. 1992 Oct;73(Pt 10):2763–2766. [PubMed]
  • Ilyina TV, Koonin EV. Conserved sequence motifs in the initiator proteins for rolling circle DNA replication encoded by diverse replicons from eubacteria, eucaryotes and archaebacteria. Nucleic Acids Res. 1992 Jul 11;20(13):3279–3285. [PMC free article] [PubMed]
  • Gorbalenya AE, Koonin EV, Wolf YI. A new superfamily of putative NTP-binding domains encoded by genomes of small DNA and RNA viruses. FEBS Lett. 1990 Mar 12;262(1):145–148. [PubMed]
  • Kenton A, Khashoggi A, Parokonny A, Bennett MD, Lichtenstein C. Chromosomal location of endogenous geminivirus-related DNA sequences in Nicotiana tabacum L. Chromosome Res. 1995 Sep;3(6):346–350. [PubMed]
  • Needleman SB, Wunsch CD. A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol. 1970 Mar;48(3):443–453. [PubMed]
  • Lipman DJ, Pearson WR. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. [PubMed]
  • Stanley J, Frischmuth T, Ellwood S. Defective viral DNA ameliorates symptoms of geminivirus infection in transgenic plants. Proc Natl Acad Sci U S A. 1990 Aug;87(16):6291–6295. [PMC free article] [PubMed]

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