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EMBO J. Apr 1989; 8(4): 1205–1212.
PMCID: PMC400935

Autoregulatory and gap gene response elements of the even-skipped promoter of Drosophila.

Abstract

The pair-rule gene even-skipped (eve) plays a key role in the regulatory hierarchy governing segmentation in Drosophila. Here we describe the use of P-transformation and eve promoter fusions to identify cis elements that regulate the periodic seven-stripe eve pattern. A distal region of the eve promoter, located between -5.9 and -5.2 kb, controls autoregulation. Sequences from this region will induce striped expression of a heterologous hsp70 basal promoter in the presence, but not absence, of endogenous eve+ products. Autoregulatory activity was localized to a 200-bp region of the distal eve promoter. We also provide evidence that individual eve expression stripes are regulated by separate cis sequences. eve promoter sequences located between -4.7 and -3 kb upstream of the transcription start site are important for the initiation of stripe 3, whereas sequences between -1.7 and -0.4 kb are needed for stripes 2 and 7. It is possible that these latter regions are directly regulated by the products of gap genes.

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Selected References

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  • Hiromi Y, Gehring WJ. Regulation and function of the Drosophila segmentation gene fushi tarazu. Cell. 1987 Sep 11;50(6):963–974. [PubMed]
  • Hiromi Y, Kuroiwa A, Gehring WJ. Control elements of the Drosophila segmentation gene fushi tarazu. Cell. 1985 Dec;43(3 Pt 2):603–613. [PubMed]
  • Hoey T, Levine M. Divergent homeo box proteins recognize similar DNA sequences in Drosophila. Nature. 1988 Apr 28;332(6167):858–861. [PubMed]
  • Howard K, Ingham P. Regulatory interactions between the segmentation genes fushi tarazu, hairy, and engrailed in the Drosophila blastoderm. Cell. 1986 Mar 28;44(6):949–957. [PubMed]
  • Howard K, Ingham P, Rushlow C. Region-specific alleles of the Drosophila segmentation gene hairy. Genes Dev. 1988 Aug;2(8):1037–1046. [PubMed]
  • Ingham PW. The molecular genetics of embryonic pattern formation in Drosophila. Nature. 1988 Sep 1;335(6185):25–34. [PubMed]
  • Ingham PW, Ish-Horowicz D, Howard KR. Correlative changes in homoeotic and segmentation gene expression in Krüppel mutant embryos of Drosophila. EMBO J. 1986 Jul;5(7):1659–1665. [PMC free article] [PubMed]
  • Ingham PW, Baker NE, Martinez-Arias A. Regulation of segment polarity genes in the Drosophila blastoderm by fushi tarazu and even skipped. Nature. 1988 Jan 7;331(6151):73–75. [PubMed]
  • Karess RE, Rubin GM. Analysis of P transposable element functions in Drosophila. Cell. 1984 Aug;38(1):135–146. [PubMed]
  • Kornberg T. Engrailed: a gene controlling compartment and segment formation in Drosophila. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1095–1099. [PMC free article] [PubMed]
  • Kornberg T, Sidén I, O'Farrell P, Simon M. The engrailed locus of Drosophila: in situ localization of transcripts reveals compartment-specific expression. Cell. 1985 Jan;40(1):45–53. [PubMed]
  • Lawrence PA, Morata G. Compartments in the wing of Drosophila: a study of the engrailed gene. Dev Biol. 1976 Jun;50(2):321–337. [PubMed]
  • Lawrence PA, Johnston P, Macdonald P, Struhl G. Borders of parasegments in Drosophila embryos are delimited by the fushi tarazu and even-skipped genes. Nature. 328(6129):440–442. [PubMed]
  • Macdonald PM, Ingham P, Struhl G. Isolation, structure, and expression of even-skipped: a second pair-rule gene of Drosophila containing a homeo box. Cell. 1986 Dec 5;47(5):721–734. [PubMed]
  • McGinnis W, Levine MS, Hafen E, Kuroiwa A, Gehring WJ. A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes. Nature. 308(5958):428–433. [PubMed]
  • Meinhardt H. Hierarchical inductions of cell states: a model for segmentation in Drosophila. J Cell Sci Suppl. 1986;4:357–381. [PubMed]
  • Mismer D, Rubin GM. Analysis of the promoter of the ninaE opsin gene in Drosophila melanogaster. Genetics. 1987 Aug;116(4):565–578. [PMC free article] [PubMed]
  • Nüsslein-Volhard C, Wieschaus E. Mutations affecting segment number and polarity in Drosophila. Nature. 1980 Oct 30;287(5785):795–801. [PubMed]
  • Nüsslein-Volhard C, Kluding H, Jürgens G. Genes affecting the segmental subdivision of the Drosophila embryo. Cold Spring Harb Symp Quant Biol. 1985;50:145–154. [PubMed]
  • Rubin GM, Spradling AC. Genetic transformation of Drosophila with transposable element vectors. Science. 1982 Oct 22;218(4570):348–353. [PubMed]
  • Rubin GM, Spradling AC. Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acids Res. 1983 Sep 24;11(18):6341–6351. [PMC free article] [PubMed]
  • Scott MP, Weiner AJ. Structural relationships among genes that control development: sequence homology between the Antennapedia, Ultrabithorax, and fushi tarazu loci of Drosophila. Proc Natl Acad Sci U S A. 1984 Jul;81(13):4115–4119. [PMC free article] [PubMed]
  • Akam M. The molecular basis for metameric pattern in the Drosophila embryo. Development. 1987 Sep;101(1):1–22. [PubMed]
  • Carroll SB, Scott MP. Zygotically active genes that affect the spatial expression of the fushi tarazu segmentation gene during early Drosophila embryogenesis. Cell. 1986 Apr 11;45(1):113–126. [PubMed]
  • Carroll SB, Laughon A, Thalley BS. Expression, function, and regulation of the hairy segmentation protein in the Drosophila embryo. Genes Dev. 1988 Jul;2(7):883–890. [PubMed]
  • DiNardo S, O'Farrell PH. Establishment and refinement of segmental pattern in the Drosophila embryo: spatial control of engrailed expression by pair-rule genes. Genes Dev. 1987 Dec;1(10):1212–1225. [PubMed]
  • DiNardo S, Kuner JM, Theis J, O'Farrell PH. Development of embryonic pattern in D. melanogaster as revealed by accumulation of the nuclear engrailed protein. Cell. 1985 Nov;43(1):59–69. [PMC free article] [PubMed]
  • Fjose A, McGinnis WJ, Gehring WJ. Isolation of a homoeo box-containing gene from the engrailed region of Drosophila and the spatial distribution of its transcripts. Nature. 1985 Jan 24;313(6000):284–289. [PubMed]
  • Frasch M, Levine M. Complementary patterns of even-skipped and fushi tarazu expression involve their differential regulation by a common set of segmentation genes in Drosophila. Genes Dev. 1987 Nov;1(9):981–995. [PubMed]
  • Frasch M, Hoey T, Rushlow C, Doyle H, Levine M. Characterization and localization of the even-skipped protein of Drosophila. EMBO J. 1987 Mar;6(3):749–759. [PMC free article] [PubMed]
  • Frasch M, Warrior R, Tugwood J, Levine M. Molecular analysis of even-skipped mutants in Drosophila development. Genes Dev. 1988 Dec;2(12B):1824–1838. [PubMed]
  • Gergen JP, Butler BA. Isolation of the Drosophila segmentation gene runt and analysis of its expression during embryogenesis. Genes Dev. 1988 Sep;2(9):1179–1193. [PubMed]
  • Kuroiwa A, Hafen E, Gehring WJ. Cloning and transcriptional analysis of the segmentation gene fushi tarazu of Drosophila. Cell. 1984 Jul;37(3):825–831. [PubMed]
  • Harding K, Rushlow C, Doyle HJ, Hoey T, Levine M. Cross-regulatory interactions among pair-rule genes in Drosophila. Science. 1986 Aug 29;233(4767):953–959. [PubMed]

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