• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of embojLink to Publisher's site
EMBO J. Mar 1, 1995; 14(5): 1015–1023.
PMCID: PMC398173

Crystal structure of casein kinase-1, a phosphate-directed protein kinase.

Abstract

The structure of a truncated variant of casein kinase-1 from Schizosaccharomyces pombe, has been determined in complex with MgATP at 2.0 A resolution. The model resembles the 'closed', ATP-bound conformations of the cyclin-dependent kinase 2 and the cAMP-dependent protein kinase, with clear differences in the structure of surface loops that impart unique features to casein kinase-1. The structure is of unphosphorylated, active conformation of casein kinase-1 and the peptide-binding site is fully accessible to substrate.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.7M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Barford D, Johnson LN. The allosteric transition of glycogen phosphorylase. Nature. 1989 Aug 24;340(6235):609–616. [PubMed]
  • Bossemeyer D, Engh RA, Kinzel V, Ponstingl H, Huber R. Phosphotransferase and substrate binding mechanism of the cAMP-dependent protein kinase catalytic subunit from porcine heart as deduced from the 2.0 A structure of the complex with Mn2+ adenylyl imidodiphosphate and inhibitor peptide PKI(5-24). EMBO J. 1993 Mar;12(3):849–859. [PMC free article] [PubMed]
  • Brockman JL, Anderson RA. Casein kinase I is regulated by phosphatidylinositol 4,5-bisphosphate in native membranes. J Biol Chem. 1991 Feb 5;266(4):2508–2512. [PubMed]
  • Brockman JL, Gross SD, Sussman MR, Anderson RA. Cell cycle-dependent localization of casein kinase I to mitotic spindles. Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9454–9458. [PMC free article] [PubMed]
  • Carmel G, Leichus B, Cheng X, Patterson SD, Mirza U, Chait BT, Kuret J. Expression, purification, crystallization, and preliminary x-ray analysis of casein kinase-1 from Schizosaccharomyces pombe. J Biol Chem. 1994 Mar 11;269(10):7304–7309. [PubMed]
  • Cegielska A, Virshup DM. Control of simian virus 40 DNA replication by the HeLa cell nuclear kinase casein kinase I. Mol Cell Biol. 1993 Feb;13(2):1202–1211. [PMC free article] [PubMed]
  • Chijiwa T, Hagiwara M, Hidaka H. A newly synthesized selective casein kinase I inhibitor, N-(2-aminoethyl)-5-chloroisoquinoline-8-sulfonamide, and affinity purification of casein kinase I from bovine testis. J Biol Chem. 1989 Mar 25;264(9):4924–4927. [PubMed]
  • Cook PF, Neville ME, Jr, Vrana KE, Hartl FT, Roskoski R., Jr Adenosine cyclic 3',5'-monophosphate dependent protein kinase: kinetic mechanism for the bovine skeletal muscle catalytic subunit. Biochemistry. 1982 Nov 9;21(23):5794–5799. [PubMed]
  • De Bondt HL, Rosenblatt J, Jancarik J, Jones HD, Morgan DO, Kim SH. Crystal structure of cyclin-dependent kinase 2. Nature. 1993 Jun 17;363(6430):595–602. [PubMed]
  • de Groot RP, den Hertog J, Vandenheede JR, Goris J, Sassone-Corsi P. Multiple and cooperative phosphorylation events regulate the CREM activator function. EMBO J. 1993 Oct;12(10):3903–3911. [PMC free article] [PubMed]
  • Dhillon N, Hoekstra MF. Characterization of two protein kinases from Schizosaccharomyces pombe involved in the regulation of DNA repair. EMBO J. 1994 Jun 15;13(12):2777–2788. [PMC free article] [PubMed]
  • Flotow H, Roach PJ. Role of acidic residues as substrate determinants for casein kinase I. J Biol Chem. 1991 Feb 25;266(6):3724–3727. [PubMed]
  • Flotow H, Graves PR, Wang AQ, Fiol CJ, Roeske RW, Roach PJ. Phosphate groups as substrate determinants for casein kinase I action. J Biol Chem. 1990 Aug 25;265(24):14264–14269. [PubMed]
  • Floyd CC, Grant P, Gallant PE, Pant HC. Principal neurofilament-associated protein kinase in squid axoplasm is related to casein kinase I. J Biol Chem. 1991 Mar 15;266(8):4987–4994. [PubMed]
  • Goldstein LS. The kinesin superfamily: tails of functional redundancy. Trends Cell Biol. 1991 Oct;1(4):93–98. [PubMed]
  • Hanks SK, Quinn AM, Hunter T. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science. 1988 Jul 1;241(4861):42–52. [PubMed]
  • Hoekstra MF, Liskay RM, Ou AC, DeMaggio AJ, Burbee DG, Heffron F. HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA. Science. 1991 Aug 30;253(5023):1031–1034. [PubMed]
  • Issinger OG. Casein kinases: pleiotropic mediators of cellular regulation. Pharmacol Ther. 1993;59(1):1–30. [PubMed]
  • Jones TA, Zou JY, Cowan SW, Kjeldgaard M. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr A. 1991 Mar 1;47(Pt 2):110–119. [PubMed]
  • Kabsch W, Sander C. Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features. Biopolymers. 1983 Dec;22(12):2577–2637. [PubMed]
  • Karlsson R, Zheng J, Xuong N, Taylor SS, Sowadski JM. Structure of the mammalian catalytic subunit of cAMP-dependent protein kinase and an inhibitor peptide displays an open conformation. Acta Crystallogr D Biol Crystallogr. 1993 Jul 1;49(Pt 4):381–388. [PubMed]
  • Kearney PH, Ebert M, Kuret J. Molecular cloning and sequence analysis of two novel fission yeast casein kinase-1 isoforms. Biochem Biophys Res Commun. 1994 Aug 30;203(1):231–236. [PubMed]
  • Knighton DR, Zheng JH, Ten Eyck LF, Ashford VA, Xuong NH, Taylor SS, Sowadski JM. Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science. 1991 Jul 26;253(5018):407–414. [PubMed]
  • Knighton DR, Zheng JH, Ten Eyck LF, Xuong NH, Taylor SS, Sowadski JM. Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science. 1991 Jul 26;253(5018):414–420. [PubMed]
  • Kong CT, Cook PF. Isotope partitioning in the adenosine 3',5'-monophosphate dependent protein kinase reaction indicates a steady-state random kinetic mechanism. Biochemistry. 1988 Jun 28;27(13):4795–4799. [PubMed]
  • Kuret J, Woodgett JR, Cohen P. Multisite phosphorylation of glycogen synthase from rabbit skeletal muscle. Identification of the sites phosphorylated by casein kinase-I. Eur J Biochem. 1985 Aug 15;151(1):39–48. [PubMed]
  • Meggio F, Perich JW, Reynolds EC, Pinna LA. A synthetic beta-casein phosphopeptide and analogues as model substrates for casein kinase-1, a ubiquitous, phosphate directed protein kinase. FEBS Lett. 1991 Jun 3;283(2):303–306. [PubMed]
  • Milne DM, Palmer RH, Campbell DG, Meek DW. Phosphorylation of the p53 tumour-suppressor protein at three N-terminal sites by a novel casein kinase I-like enzyme. Oncogene. 1992 Jul;7(7):1361–1369. [PubMed]
  • Morgan DO, De Bondt HL. Protein kinase regulation: insights from crystal structure analysis. Curr Opin Cell Biol. 1994 Apr;6(2):239–246. [PubMed]
  • Nicholls A, Sharp KA, Honig B. Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins. 1991;11(4):281–296. [PubMed]
  • RAMACHANDRAN GN, RAMAKRISHNAN C, SASISEKHARAN V. Stereochemistry of polypeptide chain configurations. J Mol Biol. 1963 Jul;7:95–99. [PubMed]
  • Roach PJ. Control of glycogen synthase by hierarchal protein phosphorylation. FASEB J. 1990 Sep;4(12):2961–2968. [PubMed]
  • Robinson RG, Preston DF, Spicer JA, Baxter KG. Radionuclide therapy of intractable bone pain: emphasis on strontium-89. Semin Nucl Med. 1992 Jan;22(1):28–32. [PubMed]
  • Roof DM, Meluh PB, Rose MD. Kinesin-related proteins required for assembly of the mitotic spindle. J Cell Biol. 1992 Jul;118(1):95–108. [PMC free article] [PubMed]
  • Solomon MJ, Harper JW, Shuttleworth J. CAK, the p34cdc2 activating kinase, contains a protein identical or closely related to p40MO15. EMBO J. 1993 Aug;12(8):3133–3142. [PMC free article] [PubMed]
  • Taylor SS, Radzio-Andzelm E. Three protein kinase structures define a common motif. Structure. 1994 May 15;2(5):345–355. [PubMed]
  • Tuazon PT, Traugh JA. Casein kinase I and II--multipotential serine protein kinases: structure, function, and regulation. Adv Second Messenger Phosphoprotein Res. 1991;23:123–164. [PubMed]
  • Umphress JL, Tuazon PT, Chen CJ, Traugh JA. Determinants on simian virus 40 large T antigen are important for recognition and phosphorylation by casein kinase I. Eur J Biochem. 1992 Jan 15;203(1-2):239–243. [PubMed]
  • Vancura A, Sessler A, Leichus B, Kuret J. A prenylation motif is required for plasma membrane localization and biochemical function of casein kinase I in budding yeast. J Biol Chem. 1994 Jul 29;269(30):19271–19278. [PubMed]
  • Vincent IJ, Davies P. A protein kinase associated with paired helical filaments in Alzheimer disease. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2878–2882. [PMC free article] [PubMed]
  • Volz K, Matsumura P. Crystal structure of Escherichia coli CheY refined at 1.7-A resolution. J Biol Chem. 1991 Aug 15;266(23):15511–15519. [PubMed]
  • Wang BC. Resolution of phase ambiguity in macromolecular crystallography. Methods Enzymol. 1985;115:90–112. [PubMed]
  • Wang PC, Vancura A, Mitcheson TG, Kuret J. Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1. Mol Biol Cell. 1992 Mar;3(3):275–286. [PMC free article] [PubMed]
  • Wang PC, Vancura A, Desai A, Carmel G, Kuret J. Cytoplasmic forms of fission yeast casein kinase-1 associate primarily with the particulate fraction of the cell. J Biol Chem. 1994 Apr 22;269(16):12014–12023. [PubMed]
  • Zheng J, Knighton DR, ten Eyck LF, Karlsson R, Xuong N, Taylor SS, Sowadski JM. Crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MgATP and peptide inhibitor. Biochemistry. 1993 Mar 9;32(9):2154–2161. [PubMed]

Articles from The EMBO Journal are provided here courtesy of The European Molecular Biology Organization

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...