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Proc Natl Acad Sci U S A. Apr 16, 1996; 93(8): 3450–3454.

Development of a bovine X chromosome linkage group and painting probes to assess cattle, sheep, and goat X chromosome segment homologies.


The X chromosome linkage group is conserved in placental mammals. However, X chromosome morphological differences, due to internal chromosome rearrangements, exist among mammalian species. We have developed bovine chromosome painting probes for Xp and Xq to assess segment homologies between the submetacentric bovine X chromosome and the acrocentric sheep and goat X chromosomes. These painting probes and their corresponding DNA libraries were developed by chromosome micromanipulation, DNA micropurification, microcloning, and PCR amplification. The bovine Xp painting probe identified an interstitially located homologous segment in the sheep and goat Xq region, most probably resulting from chromosome inversion. Ten type II (microsatellite) markers obtained from the bovine Xq library and five other X chromosome assigned, but unlinked, markers were used to generate a linkage map for Xq spanning 89.4 centimorgans. The chromosome painting probes and molecular markers generated in this study would be useful for comparative mapping and tracing of internal X chromosome rearrangements in all ruminant species and would contribute to the understanding of mammalian sex chromosome evolution.

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  • O'Brien SJ. Mammalian genome mapping: lessons and prospects. Curr Opin Genet Dev. 1991 Jun;1(1):105–111. [PubMed]
  • Bumstead N, Palyga J. A preliminary linkage map of the chicken genome. Genomics. 1992 Jul;13(3):690–697. [PubMed]
  • Bishop MD, Kappes SM, Keele JW, Stone RT, Sunden SL, Hawkins GA, Toldo SS, Fries R, Grosz MD, Yoo J, et al. A genetic linkage map for cattle. Genetics. 1994 Feb;136(2):619–639. [PMC free article] [PubMed]
  • Crawford AM, Dodds KG, Ede AJ, Pierson CA, Montgomery GW, Garmonsway HG, Beattie AE, Davies K, Maddox JF, Kappes SW, et al. An autosomal genetic linkage map of the sheep genome. Genetics. 1995 Jun;140(2):703–724. [PMC free article] [PubMed]
  • Ellegren H, Chowdhary BP, Johansson M, Marklund L, Fredholm M, Gustavsson I, Andersson L. A primary linkage map of the porcine genome reveals a low rate of genetic recombination. Genetics. 1994 Aug;137(4):1089–1100. [PMC free article] [PubMed]
  • Levin I, Santangelo L, Cheng H, Crittenden LB, Dodgson JB. An autosomal genetic linkage map of the chicken. J Hered. 1994 Mar-Apr;85(2):79–85. [PubMed]
  • Rohrer GA, Alexander LJ, Keele JW, Smith TP, Beattie CW. A microsatellite linkage map of the porcine genome. Genetics. 1994 Jan;136(1):231–245. [PMC free article] [PubMed]
  • Eggen A, Fries R. An integrated cytogenetic and meiotic map of the bovine genome. Anim Genet. 1995 Aug;26(4):215–236. [PubMed]
  • O'Brien SJ, Womack JE, Lyons LA, Moore KJ, Jenkins NA, Copeland NG. Anchored reference loci for comparative genome mapping in mammals. Nat Genet. 1993 Feb;3(2):103–112. [PubMed]
  • Lüdecke HJ, Senger G, Claussen U, Horsthemke B. Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification. Nature. 1989 Mar 23;338(6213):348–350. [PubMed]
  • Saunders RD, Glover DM, Ashburner M, Siden-Kiamos I, Louis C, Monastirioti M, Savakis C, Kafatos F. PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning. Nucleic Acids Res. 1989 Nov 25;17(22):9027–9037. [PMC free article] [PubMed]
  • Di Berardino D, Iannuzzi L. Detailed description of R-banded bovine chromosomes. J Hered. 1982 Nov-Dec;73(6):434–438. [PubMed]
  • Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet. 1988 Nov;80(3):224–234. [PubMed]
  • Lichter P, Tang CJ, Call K, Hermanson G, Evans GA, Housman D, Ward DC. High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science. 1990 Jan 5;247(4938):64–69. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Kappes SM, Bishop MD, Keele JW, Penedo MC, Hines HC, Grosz MD, Hawkins GA, Stone RT, Sunden SL, Beattie CW. Linkage of bovine erythrocyte antigen loci B, C, L, S, Z, R' and T' and the serum protein loci post-transferrin 2 (PTF 2), vitamin D binding protein (GC) and albumin (ALB) to DNA microsatellite markers. Anim Genet. 1994 Jun;25(3):133–140. [PubMed]
  • Keele JW, Wray JE, Behrens DW, Rohrer GA, Sunden SL, Kappes SM, Bishop MD, Stone RT, Alexander LJ, Beattie CW. A conceptual database model for genomic research. J Comput Biol. 1994 Spring;1(1):65–76. [PubMed]
  • Lander ES, Green P. Construction of multilocus genetic linkage maps in humans. Proc Natl Acad Sci U S A. 1987 Apr;84(8):2363–2367. [PMC free article] [PubMed]
  • Goudarzi K, Ciampolini R, Vaiman D, Leveziel H. A new bovine dinucleotide repeat microsatellite: microsatellite INRA 18. Anim Genet. 1993 Jun;24(3):221–221. [PubMed]
  • Hayes H, Petit E, Dutrillaux B. Comparison of RBG-banded karyotypes of cattle, sheep, and goats. Cytogenet Cell Genet. 1991;57(1):51–55. [PubMed]
  • Kaftanovskaya HM, Serov OL. High-resolution GTG-banded chromosomes of cattle, sheep, and goat: a comparative study. J Hered. 1994 Sep-Oct;85(5):395–400. [PubMed]

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