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Proc Natl Acad Sci U S A. 1996 April 16; 93(8): 3346–3351. | PMCID: PMC39610 |
Ecdysone-inducible gene expression in mammalian cells and transgenic mice. D No, T P Yao, and R M Evans Howard Hughes Medical Institute, Salk Institute for Biomedical Studies, La Jolla, CA 92037, USA. Abstract During metamorphosis of Drosophila melanogaster, a cascade of morphological changes is triggered by the steroid hormone 20-OH ecdysone via the ecdysone receptor, a member of the nuclear receptor superfamily. In this report, we have transferred insect hormone responsiveness to mammalian cells by the stable expression of a modified ecdysone receptor that regulates an optimized ecdysone responsive promoter. Inductions reaching 4 orders of magnitude have been achieved upon treatment with hormone. Transgenic mice expressing the modified ecdysone receptor can activate an integrated ecdysone responsive promoter upon administration of hormone. A comparison of tetracycline-based and ecdysone-based inducible systems reveals the ecdysone regulatory system exhibits lower basal activity and higher inducibility. Since ecdysone administration has no apparent effect on mammals, its use for regulating genes should be excellent for transient inducible expression of any gene in transgenic mice and for gene therapy. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.7M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - Jaenisch R. Transgenic animals. Science. 1988 Jun 10;240(4858):1468–1474. [PubMed]
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