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Proc Natl Acad Sci U S A. Apr 30, 1996; 93(9): 3859–3864.
PMCID: PMC39449

Detection of a major gene for resistance to fusiform rust disease in loblolly pine by genomic mapping.

Abstract

Genomic mapping has been used to identify a region of the host genome that determines resistance to fusiform rust disease in loblolly pine where no discrete, simply inherited resistance factors had been previously found by conventional genetic analysis over four decades. A resistance locus, behaving as a single dominant gene, was mapped by association with genetic markers, even though the disease phenotype deviated from the expected Mendelian ratio. The complexity of forest pathosystems and the limitations of genetic analysis, based solely on phenotype, had led to an assumption that effective long-term disease resistance in trees should be polygenic. However, our data show that effective long-term resistance can be obtained from a single qualitative resistance gene, despite the presence of virulence in the pathogen population. Therefore, disease resistance in this endemic coevolved forest pathosystem is not exclusively polygenic. Genomic mapping now provides a powerful tool for characterizing the genetic basis of host pathogen interactions in forest trees and other undomesticated, organisms, where conventional genetic analysis often is limited or not feasible.

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Selected References

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  • Kinloch BB, Jr, Parks GK, Fowler CW. White pine blister rust: simply inherited resistance in sugar pine. Science. 1970 Jan 9;167(3915):193–195. [PubMed]
  • Devey ME, Delfino-Mix A, Kinloch BB, Jr, Neale DB. Random amplified polymorphic DNA markers tightly linked to a gene for resistance to white pine blister rust in sugar pine. Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2066–2070. [PMC free article] [PubMed]
  • Bradshaw HD, Jr, Stettler RF. Molecular genetics of growth and development in populus. IV. Mapping QTLs with large effects on growth, form, and phenology traits in a forest tree. Genetics. 1995 Feb;139(2):963–973. [PMC free article] [PubMed]
  • Groover A, Devey M, Fiddler T, Lee J, Megraw R, Mitchel-Olds T, Sherman B, Vujcic S, Williams C, Neale D. Identification of quantitative trait loci influencing wood specific gravity in an outbred pedigree of loblolly pine. Genetics. 1994 Dec;138(4):1293–1300. [PMC free article] [PubMed]
  • Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990 Nov 25;18(22):6531–6535. [PMC free article] [PubMed]
  • Grattapaglia D, Sederoff R. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics. 1994 Aug;137(4):1121–1137. [PMC free article] [PubMed]
  • Lander ES, Schork NJ. Genetic dissection of complex traits. Science. 1994 Sep 30;265(5181):2037–2048. [PubMed]
  • Hulbert SH, Ilott TW, Legg EJ, Lincoln SE, Lander ES, Michelmore RW. Genetic analysis of the fungus, Bremia lactucae, using restriction fragment length polymorphisms. Genetics. 1988 Dec;120(4):947–958. [PMC free article] [PubMed]
  • Michelmore RW, Paran I, Kesseli RV. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9828–9832. [PMC free article] [PubMed]
  • Hamilton WD, Axelrod R, Tanese R. Sexual reproduction as an adaptation to resist parasites (a review). Proc Natl Acad Sci U S A. 1990 May;87(9):3566–3573. [PMC free article] [PubMed]

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