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EMBO J. Aug 15, 1995; 14(16): 3925–3936.
PMCID: PMC394471

Dominant negative mutants of the Cdc2 kinase uncouple cell division from iterative plant development.

Abstract

Because plant cells do not move and are surrounded by a rigid cell wall, cell division rates and patterns are believed to be directly responsible for generating new structures throughout development. To study the relationship between cell division and morphogenesis, transgenic tobacco and Arabidopsis plants were constructed expressing dominant mutations in a key regulator of the Arabidopsis cell cycle, the Cdc2a kinase. Plants constitutively overproducing the wild-type Cdc2a or the mutant form predicted to accelerate the cell cycle did not exhibit a significantly altered development. In contrast, a mutation expected to arrest the cell cycle abolished cell division when expressed in Arabidopsis, whereas some tobacco plants constitutively producing this mutant protein were recovered. These plants had a reduced histone H1 kinase activity and contained considerably fewer cells. These cells were, however, much larger and underwent normal differentiation. Morphogenesis, histogenesis and developmental timing were unaffected. The results indicate that, in plants, the developmental controls defining shape can act independently from cell division rates.

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Selected References

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  • Amon A, Surana U, Muroff I, Nasmyth K. Regulation of p34CDC28 tyrosine phosphorylation is not required for entry into mitosis in S. cerevisiae. Nature. 1992 Jan 23;355(6358):368–371. [PubMed]
  • Atherton-Fessler S, Hannig G, Piwnica-Worms H. Reversible tyrosine phosphorylation and cell cycle control. Semin Cell Biol. 1993 Dec;4(6):433–442. [PubMed]
  • Azzi L, Meijer L, Reed SI, Pidikiti R, Tung HY. Interaction between the cell-cycle-control proteins p34cdc2 and p9CKShs2. Evidence for two cooperative binding domains in p9CKShs2. Eur J Biochem. 1992 Feb 1;203(3):353–360. [PubMed]
  • Benfey PN, Ren L, Chua NH. Tissue-specific expression from CaMV 35S enhancer subdomains in early stages of plant development. EMBO J. 1990 Jun;9(6):1677–1684. [PMC free article] [PubMed]
  • Bergounioux C, Brown SC. Plant cell cycle analysis with isolated nuclei. Methods Cell Biol. 1990;33:563–573. [PubMed]
  • Booher R, Beach D. Site-specific mutagenesis of cdc2+, a cell cycle control gene of the fission yeast Schizosaccharomyces pombe. Mol Cell Biol. 1986 Oct;6(10):3523–3530. [PMC free article] [PubMed]
  • Busturia A, Lawrence PA. Regulation of cell number in Drosophila. Nature. 1994 Aug 18;370(6490):561–563. [PubMed]
  • De Bondt HL, Rosenblatt J, Jancarik J, Jones HD, Morgan DO, Kim SH. Crystal structure of cyclin-dependent kinase 2. Nature. 1993 Jun 17;363(6430):595–602. [PubMed]
  • Durkacz B, Carr A, Nurse P. Transcription of the cdc2 cell cycle control gene of the fission yeast Schizosaccharomyces pombe. EMBO J. 1986 Feb;5(2):369–373. [PMC free article] [PubMed]
  • Ferreira PC, Hemerly AS, Villarroel R, Van Montagu M, Inzé D. The Arabidopsis functional homolog of the p34cdc2 protein kinase. Plant Cell. 1991 May;3(5):531–540. [PMC free article] [PubMed]
  • Gould KL, Nurse P. Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis. Nature. 1989 Nov 2;342(6245):39–45. [PubMed]
  • Hemerly AS, Ferreira P, de Almeida Engler J, Van Montagu M, Engler G, Inzé D. cdc2a expression in Arabidopsis is linked with competence for cell division. Plant Cell. 1993 Dec;5(12):1711–1723. [PMC free article] [PubMed]
  • Hérouart D, Van Montagu M, Inzé D. Developmental and environmental regulation of the Nicotiana plumbaginifolia cytosolic Cu/Zn-superoxide dismutase promoter in transgenic tobacco. Plant Physiol. 1994 Mar;104(3):873–880. [PMC free article] [PubMed]
  • A simple and general method for transferring genes into plants. Science. 1985 Mar 8;227(4691):1229–1231. [PubMed]
  • Imajuku Y, Hirayama T, Endoh H, Oka A. Exon-intron organization of the Arabidopsis thaliana protein kinase genes CDC2a and CDC2b. FEBS Lett. 1992 Jun 8;304(1):73–77. [PubMed]
  • Jacobs T. Control of the cell cycle. Dev Biol. 1992 Sep;153(1):1–15. [PubMed]
  • Jürgens G. Pattern formation in the flowering plant embryo. Curr Opin Genet Dev. 1992 Aug;2(4):567–570. [PubMed]
  • Krek W, Nigg EA. Differential phosphorylation of vertebrate p34cdc2 kinase at the G1/S and G2/M transitions of the cell cycle: identification of major phosphorylation sites. EMBO J. 1991 Feb;10(2):305–316. [PMC free article] [PubMed]
  • Krek W, Nigg EA. Mutations of p34cdc2 phosphorylation sites induce premature mitotic events in HeLa cells: evidence for a double block to p34cdc2 kinase activation in vertebrates. EMBO J. 1991 Nov;10(11):3331–3341. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Landt O, Grunert HP, Hahn U. A general method for rapid site-directed mutagenesis using the polymerase chain reaction. Gene. 1990 Nov 30;96(1):125–128. [PubMed]
  • Martinez MC, Jørgensen JE, Lawton MA, Lamb CJ, Doerner PW. Spatial pattern of cdc2 expression in relation to meristem activity and cell proliferation during plant development. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7360–7364. [PMC free article] [PubMed]
  • Maundrell K. nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine. J Biol Chem. 1990 Jul 5;265(19):10857–10864. [PubMed]
  • Maundrell K. Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene. 1993 Jan 15;123(1):127–130. [PubMed]
  • Mendenhall MD, Richardson HE, Reed SI. Dominant negative protein kinase mutations that confer a G1 arrest phenotype. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4426–4430. [PMC free article] [PubMed]
  • Murray AW. Cell-cycle control: turning on mitosis. Curr Biol. 1993 May 1;3(5):291–293. [PubMed]
  • Norbury C, Blow J, Nurse P. Regulatory phosphorylation of the p34cdc2 protein kinase in vertebrates. EMBO J. 1991 Nov;10(11):3321–3329. [PMC free article] [PubMed]
  • Odell JT, Nagy F, Chua NH. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature. 313(6005):810–812. [PubMed]
  • Okazaki K, Okazaki N, Kume K, Jinno S, Tanaka K, Okayama H. High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res. 1990 Nov 25;18(22):6485–6489. [PMC free article] [PubMed]
  • Pines J. Clear as crystal? Curr Biol. 1993 Aug 1;3(8):544–547. [PubMed]
  • Solomon MJ. Activation of the various cyclin/cdc2 protein kinases. Curr Opin Cell Biol. 1993 Apr;5(2):180–186. [PubMed]
  • Sorger PK, Murray AW. S-phase feedback control in budding yeast independent of tyrosine phosphorylation of p34cdc28. Nature. 1992 Jan 23;355(6358):365–368. [PubMed]
  • Valvekens D, Montagu MV, Van Lijsebettens M. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536–5540. [PMC free article] [PubMed]
  • van den Heuvel S, Harlow E. Distinct roles for cyclin-dependent kinases in cell cycle control. Science. 1993 Dec 24;262(5142):2050–2054. [PubMed]
  • West MAL, Harada JJ. Embryogenesis in Higher Plants: An Overview. Plant Cell. 1993 Oct;5(10):1361–1369. [PMC free article] [PubMed]

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