Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Jun 1983; 80(11): 3364–3367.
PMCID: PMC394043

Transcription of repetitive sequences on Xenopus lampbrush chromosomes.


We reinvestigated the lampbrush chromosomes of Xenopus laevis and found them well suited for the study or transcription by in situ hybridization to nascent RNA transcripts. Using this technique, we analyzed the transcription of three repetitive sequences that do not show any sequence homology and that differ in their degree of interspersion. We found that they are located on different parts of the chromosomes: two are clustered, one is interspersed. All three of these sequences are transcribed at the lampbrush chromosome stage and transcripts from both strands of each sequence can be detected. The amount of transcription is apparently not proportional to the number of copies of the repetitive sequence at a given chromosomal locus, suggesting that other sequences are involved in the regulation of their transcription.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Angelier N, Lacroix JC. Complexes de transcription d'origines nucléolaire et chromosomique d'ovocytes de Pleurodeles waltlii et P. poireti (amphibiens, urodèles) Chromosoma. 1975 Aug 11;51(4):323–335. [PubMed]
  • Miller OL, Jr, Bakken AH. Morphological studies of transcription. Acta Endocrinol Suppl (Copenh) 1972;168:155–177. [PubMed]
  • Callan HG. The Croonian Lecture, 1981. Lampbrush chromosomes. Proc R Soc Lond B Biol Sci. 1982 Mar 22;214(1197):417–448. [PubMed]
  • Dumont JN. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. J Morphol. 1972 Feb;136(2):153–179. [PubMed]
  • Müller WP. The lampbrush chromosomes of Xenopus laevis (Daudin). Chromosoma. 1974;47(3):283–296. [PubMed]
  • Davidson EH, Posakony JW. Repetitive sequence transcripts in development. Nature. 1982 Jun 24;297(5868):633–635. [PubMed]
  • Diaz MO, Barsacchi-Pilone G, Mahon KA, Gall JG. Transcripts from both strands of a satellite DNA occur on lampbrush chromosome loops of the newt Notophthalmus. Cell. 1981 Jun;24(3):649–659. [PubMed]
  • Macgregor HC. In situ hybridization of highly repetitive DNA to chromosomes of Triturus cristatus. Chromosoma. 1979 Feb 13;71(1):57–64. [PubMed]
  • Varley JM, Macgregor HC, Nardi I, Andrews C, Erba HP. Cytological evidence of transcription of highly repeated DNA sequences during the lampbrush stage in Triturus cristatus carnifex. Chromosoma. 1980;80(3):289–307. [PubMed]
  • Varley JM, Macgregor HC, Erba HP. Satellite DNA is transcribed on lampbrush chromosomes. Nature. 1980 Feb 14;283(5748):686–688. [PubMed]
  • Pardue ML, Gall JG. Nucleic acid hybridization to the DNA of cytological preparations. Methods Cell Biol. 1975;10:1–16. [PubMed]
  • Pukkila PJ. Identification of the lampbrush chromosome loops which transcribe 5S ribosomal RNA in Notophthalmus (Triturus) viridescens. Chromosoma. 1975 Nov 20;53(1):71–89. [PubMed]
  • Bünemann H, Müller W. Base specific fractionation of double stranded DNA: affinity chromatography on a novel type of adsorbant. Nucleic Acids Res. 1978 Mar;5(3):1059–1074. [PMC free article] [PubMed]
  • Spohr G, Reith W, Sures I. Organization and sequence analysis of a cluster of repetitive DNA elements from Xenopus laevis. J Mol Biol. 1981 Oct 5;151(4):573–592. [PubMed]
  • Gall JG, Stephenson EC, Erba HP, Diaz MO, Barsacchi-Pilone G. Histone genes are located at the sphere loci of newt lampbrush chromosomes. Chromosoma. 1981;84(2):159–171. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...