• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Jan 1983; 80(2): 594–598.
PMCID: PMC393426

Some rat sensory neurons in culture express characteristics of differentiated pain sensory cells.

Abstract

Sensory neurons were dissociated from trigeminal ganglia or from dorsal root ganglia of rats, grown in culture, and examined for expression of properties of pain sensory cells. Many sensory neurons in culture are excited by low concentrations of capsaicin, reportedly a selective stimulus for pain sensory neurons. Many are excited by bradykinin, sensitized by prostaglandin E2, or specifically stained by an antiserum against substance P. These experiments provide a basis for the study of pain mechanisms in cell culture.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Kumazawa T, Perl ER. Primate cutaneous receptors with unmyelinated (C) fibres and their projection to the substantia gelatinosa. J Physiol (Paris) 1977 Sep;73(3):287–304. [PubMed]
  • Torebjörk HE. Afferent C units responding to mechanical, thermal and chemical stimuli in human non-glabrous skin. Acta Physiol Scand. 1974 Nov;92(3):374–390. [PubMed]
  • Lynn B, Carpenter SE. Primary afferent units from the hairy skin of the rat hind limb. Brain Res. 1982 Apr 22;238(1):29–43. [PubMed]
  • Ferreira SH. Prostaglandins, aspirin-like drugs and analgesia. Nat New Biol. 1972 Dec 13;240(102):200–203. [PubMed]
  • Beck PW, Handwerker HO. Bradykinin and serotonin effects on various types of cutaneous nerve fibers. Pflugers Arch. 1974 Mar 11;347(3):209–222. [PubMed]
  • Mense S. Nervous outflow from skeletal muscle following chemical noxious stimulation. J Physiol. 1977 May;267(1):75–88. [PMC free article] [PubMed]
  • Kumazawa T, Mizumura K. The polymodal C-fiber receptor in the muscle of the dog. Brain Res. 1976 Jan 23;101(3):589–593. [PubMed]
  • Mense S. Sensitization of group IV muscle receptors to bradykinin by 5-hydroxytryptamine and prostaglandin E2. Brain Res. 1981 Nov 23;225(1):95–105. [PubMed]
  • Pateromichelakis S, Rood JP. Prostaglandin E1-induced sensitization of A delta moderate pressure mechanoreceptors. Brain Res. 1982 Jan 28;232(1):89–96. [PubMed]
  • Chahl LA, Iggo A. The effects of bradykinin and prostaglandin E1 on rat cutaneous afferent nerve activity. Br J Pharmacol. 1977 Feb;59(2):343–347. [PMC free article] [PubMed]
  • FJALLBRANT N, IGGO A. The effect of histamine, 5-hydroxytryptamine and acetylcholine on cutaneous afferent fibres. J Physiol. 1961 May;156:578–590. [PMC free article] [PubMed]
  • PORSZASZ J, JANCSO N. Studies on the action potentials of sensory nerves in animals desensitized with capsaicine. Acta Physiol Acad Sci Hung. 1959;16:299–306. [PubMed]
  • Szolcsányi J. A pharmacological approach to elucidation of the role of different nerve fibres and receptor endings in mediation of pain. J Physiol (Paris) 1977 Sep;73(3):251–259. [PubMed]
  • Kenins P. Responses of single nerve fibres to capsaicin applied to the skin. Neurosci Lett. 1982 Mar 17;29(1):83–88. [PubMed]
  • Foster RW, Ramage AG. The action of some chemical irritants on somatosensory receptors of the cat. Neuropharmacology. 1981 Feb;20(2):191–198. [PubMed]
  • Nicoll RA, Schenker C, Leeman SE. Substance P as a transmitter candidate. Annu Rev Neurosci. 1980;3:227–268. [PubMed]
  • Furshpan EJ, MacLeish PR, O'Lague PH, Potter DD. Chemical transmission between rat sympathetic neurons and cardiac myocytes developing in microcultures: evidence for cholinergic, adrenergic, and dual-function neurons. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4225–4229. [PMC free article] [PubMed]
  • Mains RE, Patterson PH. Primary cultures of dissociated sympathetic neurons. I. Establishment of long-term growth in culture and studies of differentiated properties. J Cell Biol. 1973 Nov;59(2 Pt 1):329–345. [PMC free article] [PubMed]
  • O'Lague PH, Obata K, Claude P, Furshpan EJ, Potter DD. Evidence for cholinergic synapses between dissociated rat sympathetic neurons in cell culture. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3602–3606. [PMC free article] [PubMed]
  • Choi DW, Farb DH, Fischbach GD. Chlordiazepoxide selectively augments GABA action in spinal cord cell cultures. Nature. 1977 Sep 22;269(5626):342–344. [PubMed]
  • Sternberger LA, Hardy PH, Jr, Cuculis JJ, Meyer HG. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem. 1970 May;18(5):315–333. [PubMed]
  • Nagy JI, Hunt SP, Iversen LL, Emson PC. Biochemical and anatomical observations on the degeneration of peptide-containing primary afferent neurons after neonatal capsaicin. Neuroscience. 1981;6(10):1923–1934. [PubMed]
  • Hökfelt T, Kellerth JO, Nilsson G, Pernow B. Substance p: localization in the central nervous system and in some primary sensory neurons. Science. 1975 Nov 28;190(4217):889–890. [PubMed]
  • Takahashi T, Otsuka M. Regional distribution of substance P in the spinal cord and nerve roots of the cat and the effect of dorsal root section. Brain Res. 1975 Apr 4;87(1):1–11. [PubMed]
  • Hökfelt T, Kellerth JO, Nilsson G, Pernow B. Experimental immunohistochemical studies on the localization and distribution of substance P in cat primary sensory neurons. Brain Res. 1975 Dec 19;100(2):235–252. [PubMed]
  • Cuello AC, Del Fiacco M, Paxinos G. The central and peripheral ends of the substance P-containing sensory neurones in the rat trigeminal system. Brain Res. 1978 Sep 8;152(3):499–500. [PubMed]
  • Yaksh TL, Jessell TM, Gamse R, Mudge AW, Leeman SE. Intrathecal morphine inhibits substance P release from mammalian spinal cord in vivo. Nature. 1980 Jul 10;286(5769):155–157. [PubMed]
  • Otsuka M, Konishi S. Release of substance P-like immunoreactivity from isolated spinal cord of newborn rat. Nature. 1976 Nov 4;264(5581):83–84. [PubMed]
  • Henry JL. Effects of substance P on functionally identified units in cat spinal cord. Brain Res. 1976 Sep 24;114(3):439–451. [PubMed]
  • Randić M, Miletić V. Effect of substance P in cat dorsal horn neurones activated by noxious stimuli. Brain Res. 1977 Jun 3;128(1):164–169. [PubMed]
  • Andersen RK, Lund JP, Puil E. Enkephalin and substance P effects related to trigeminal pain. Can J Physiol Pharmacol. 1978 Apr;56(2):216–222. [PubMed]
  • Mudge AW, Leeman SE, Fischbach GD. Enkephalin inhibits release of substance P from sensory neurons in culture and decreases action potential duration. Proc Natl Acad Sci U S A. 1979 Jan;76(1):526–530. [PMC free article] [PubMed]
  • Langford LA, Coggeshall RE. Branching of sensory axons in the dorsal root and evidence for the absence of dorsal root efferent fibers. J Comp Neurol. 1979 Mar 1;184(1):193–204. [PubMed]
  • Kenins P. Identification of the unmyelinated sensory nerves which evoke plasma extravasation in response to antidromic stimulation. Neurosci Lett. 1981 Sep 1;25(2):137–141. [PubMed]
  • Lembeck F, Holzer P. Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. Naunyn Schmiedebergs Arch Pharmacol. 1979 Dec;310(2):175–183. [PubMed]
  • Lembeck F, Folkers K, Donnerer J. Analgesic effect of antagonists of substance P. Biochem Biophys Res Commun. 1981 Dec 31;103(4):1318–1321. [PubMed]
  • Rosell S, Olgart L, Gazelius B, Panopoulos P, Folkers K, Hörig J. Inhibition of antidromic and substance P-induced vasodilatation by a substance P antagonist. Acta Physiol Scand. 1981 Mar;111(3):381–382. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...