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Proc Natl Acad Sci U S A. 1977 Jan; 74(1): 268–271.
PMCID: PMC393240

Cell differentiation in Dictyostelium under submerged conditions.


Hitherto it has not been possible to obtain spore and stalk cell differentiation of the cellular slime molds in submerged cultures. It is shown here that cells, when placed in roller tubes under an atmosphere of oxygen, will form clumps and differentiate in 48-72 hr into mature spores and stalk cells. Although differentiation occurs without the normal morphogenetic movements, there is the appearance of an anteroposterior polarity of the cells in the clump. In addition to oxygen we examined a number of other factors that affect differentiation.

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Selected References

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  • Cotter DA, Raper KB. Spore germination in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1966 Sep;56(3):880–887. [PMC free article] [PubMed]
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  • SONNEBORN DR, WHITE GJ, SUSSMAN M. A mutation affecting both rate and pattern of morphogenesis in Dictyostelium discoideum. Dev Biol. 1963 Mar;6:79–93. [PubMed]
  • Bonner JT. Induction of stalk cell differentiation by cyclic AMP in the cellular slime mold Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1970 Jan;65(1):110–113. [PMC free article] [PubMed]
  • Chia WK. Induction of stalk cell differentiation by cyclic-AMP in a susceptible variant of Dictyostelium discoideum. Dev Biol. 1975 Jun;44(2):239–252. [PubMed]
  • Town CD, Gross JD, Kay RR. Cell differentiation without morphogenesis in Dictyostelium discoideum. Nature. 1976 Aug 19;262(5570):717–719. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


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