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Proc Natl Acad Sci U S A. Jan 1977; 74(1): 59–63.
PMCID: PMC393196

A protein covalently linked to poliovirus genome RNA.

Abstract

Poliovirion [32P]RNA, after digestion with RNase T2, yields mononucleotides and a labeled compound "X," which is not negatively charged at pH 5. X contains, relative to the label in virion RNA, one to two phosphates and is partially acid insoluble. It can be labeled with tritiated amino acids 3 hr after infection, is insoluble in chloroform/methanol, and can be digested with Pronase. These observations suggest that X is a protein. The protein cannot be removed from the polio genome when the RNA is (i) sedimented through a sucrose gradient in 0.5 M NaCl, (ii) heated to 100 degrees in the presence of sodium dodecyl sulfate followed by sedimentation through a sucrose gradient in 80% dimethylsulfoxide, or (iii) banded in 4 M cesium trichloroacetate. Digestion of the 32P-labeled protein with Pronase yields one major 32P-labeled product, which contains pUp. The protein migrates faster than capsid protein VP4 in a polyacrylamide gel. Our data show that the genome of poliovirus, but not poliovirus mRNA [A. Nomoto, Y. F. Lee, and E. Wimmer (1976) Proc. Natl. Acad. Sci. USA 73, 375-380], is covalently attached to a small virus-coded protein (molecular weight less than 7000), which we call VPg. VPg is probably linked to the 5' end of the polio genome. Possible functions of VPg in viral replication are discussed.

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Selected References

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  • BALTIMORE D. IN VITRO SYNTHESIS OF VIRAL RNA BY THE POLIOVIRUS RNA POLYMERASE. Proc Natl Acad Sci U S A. 1964 Mar;51:450–456. [PMC free article] [PubMed]
  • Caliguiri LA. Analysis of RNA associated with the poliovirus RNA replication complexes. Virology. 1974 Apr;58(2):526–535. [PubMed]
  • Lundquist RE, Ehrenfeld E, Maizel JV., Jr Isolation of a viral polypeptide associated with poliovirus RNA polymerase. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4773–4777. [PMC free article] [PubMed]
  • Dorsch-Häsler K, Yogo Y, Wimmer E. Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded. J Virol. 1975 Dec;16(6):1512–1517. [PMC free article] [PubMed]
  • Wimmer E. Sequence studies of poliovirus RNA. I. Characterization of the 5'-terminus. J Mol Biol. 1972 Jul 28;68(3):537–540. [PubMed]
  • Hewlett MJ, Rose JK, Baltimore D. 5'-terminal structure of poliovirus polyribosomal RNA is pUp. Proc Natl Acad Sci U S A. 1976 Feb;73(2):327–330. [PMC free article] [PubMed]
  • Nomoto A, Lee YF, Wimmer E. The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np. Proc Natl Acad Sci U S A. 1976 Feb;73(2):375–380. [PMC free article] [PubMed]
  • Fernandez-Munoz R, Darnell JE. Structural difference between the 5' termini of viral and cellular mRNA in poliovirus-infected cells: possible basis for the inhibition of host protein synthesis. J Virol. 1976 May;18(2):719–726. [PMC free article] [PubMed]
  • Lee YF, Nomoto A, Wimmer E. The genome of poliovirus is an exceptional eukaryotic mRNA. Prog Nucleic Acid Res Mol Biol. 1976;19:89–96. [PubMed]
  • Lee YF, Wimmer E. "Fingerprinting" high molecular weight RNA by two-dimensional gel electrophoresis: application to poliovirus RNA. Nucleic Acids Res. 1976 Jul;3(7):1647–1658. [PMC free article] [PubMed]
  • Cole CN, Smoler D, Wimmer E, Baltimore D. Defective interfering particles of poliovirus. I. Isolation and physical properties. J Virol. 1971 Apr;7(4):478–485. [PMC free article] [PubMed]
  • Swank RT, Munkres KD. Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate. Anal Biochem. 1971 Feb;39(2):462–477. [PubMed]
  • MANDEL B. THE EXTRACTION OF RIBONUCLEIC ACID FROM POLIOVIRUS BY TREATMENT WITH SODIUM DODECYL SULFATE. Virology. 1964 Mar;22:360–367. [PubMed]
  • Tegtmeyer P, Schwartz M, Collins JK, Rundell K. Regulation of tumor antigen synthesis by simain virus 40 gene A. J Virol. 1975 Jul;16(1):168–178. [PMC free article] [PubMed]
  • Robinson AJ, Bellett JD. A circular DNA-protein complex adenoviruses and its possible role in DNA replication. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):523–531. [PubMed]
  • Brown DT, Westphal M, Burlingham BT, Winterhoff U, Doerfler W. Structure and composition of the adenovirus type 2 core. J Virol. 1975 Aug;16(2):366–387. [PMC free article] [PubMed]
  • Kasamatsu H, Wu M. Structure of a nicked DNA-protein complex isolated from simian virus 40: covalent attachment of the protein to DNA and nick specificity. Proc Natl Acad Sci U S A. 1976 Jun;73(6):1945–1949. [PMC free article] [PubMed]

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