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Journal List > Proc Natl Acad Sci U S A > v.75(12); Dec 1978

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Proc Natl Acad Sci U S A. 1978 December; 75(12): 6064–6068.
PMCID: PMC393118
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G2 phase-specific proteins of HeLa cells.
A A Al-Bader, A Orengo, and P N Rao
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Abstract
The objective of this study was to determine if HeLa cells irreversibly arrested in G2 phase of the cell cycle by a brief exposure to a nitrosourea compound were deficient in certain proteins when compared with G2-synchronized cells. Total cellular proteins of G2-synchronized, G2-arrested, and S phase-synchronized cells were compared by two-dimensional polyacrylamide gel electrophoresis. The S phase cells differed from the G2-synchronized and G2-arrested cells by the absence of about 35 and 25 protein spots, respectively, of a total of nearly 150. At least nine protein spots in the molecular weight range of 4--5 X 10(4) that were present in the G2-synchronized cells were absent in both the G2-arrested and the S phase cells. Thus, these studies suggest that the missing proteins are probably necessary for the transition of cells from G2 phase to mitosis. Supplying the missing proteins to the G2-arrested cells by fusion with G2-synchronized cells facilitated the entry of the former into mitosis.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Tobey RA. Different drugs arrest cells at a number of distinct stages in G2. Nature. 1975 Mar 20;254(5497):245–247. [PubMed]
  • Rao AP, Rao PN. The cause of G2-arrest in Chinese hamster ovary cells treated with anticancer drugs. J Natl Cancer Inst. 1976 Nov;57(5):1139–1143. [PubMed]
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  • Rao PN, Johnson RT. Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis. Nature. 1970 Jan 10;225(5228):159–164. [PubMed]
  • Rao PN. Mitotic synchrony in mammalian cells treated with nitrous oxide at high pressure. Science. 1968 May 17;160(829):774–776. [PubMed]
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