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Proc Natl Acad Sci U S A. Nov 1978; 75(11): 5565–5569.
PMCID: PMC393007

Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).


A monoclonal antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with F9 teratocarcinoma cells is described. This antibody, which reacts with embryonal carcinoma cells of mouse and human origin and with some preimplantation stage mouse embryos, defines an embryonic stage-specific antigen. This stage-specific antigen (SSEA-1) is first detected on blastomeres of 8-cell stage embryos. Trophectodermal cells are transitorily positive; however, each cell in the inner cell mass eventually expresses this antigen.

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Selected References

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  • Jacob F. Mouse teratocarcinoma and embryonic antigens. Immunol Rev. 1977 Jan;33:3–32. [PubMed]
  • Wiley LM, Calarco PG. The effects of anti-embryo sera and their localization on the cell surface during mouse preimplantation development. Dev Biol. 1975 Dec;47(2):407–418. [PubMed]
  • Kemler R, Babinet C, Eisen H, Jacob F. Surface antigen in early differentiation. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4449–4452. [PMC free article] [PubMed]
  • Galfre G, Howe SC, Milstein C, Butcher GW, Howard JC. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. [PubMed]
  • Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. [PubMed]
  • Köhler G, Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol. 1976 Jul;6(7):511–519. [PubMed]
  • Berstine EG, Hooper ML, Grandchamp S, Ephrussi B. Alkaline phosphatase activity in mouse teratoma. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3899–3903. [PMC free article] [PubMed]
  • Jakob H, Boon T, Gaillard J, Nicolas J, Jacob F. Tératocarcinome de la spuris: isolement, culture et propriétés de cellules a potentialités multiples. Ann Microbiol (Paris) 1973 Oct;124(3):269–282. [PubMed]
  • Dewey MJ, Martin DW, Jr, Martin GR, Mintz B. Mosaic mice with teratocarcinoma-derived mutant cells deficient in hypoxanthine phosphoribosyltransferase. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5564–5568. [PMC free article] [PubMed]
  • Lehman JM, Speers WC, Swartzendruber DE, Pierce GB. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma. J Cell Physiol. 1974 Aug;84(1):13–27. [PubMed]
  • Knowles BB, Ford SR, Aden DP. Reactivity to SV40 T antigen in athymic (nude), anti-thymocyte serum-treated, and normal mice. J Immunol. 1977 Jul;119(1):79–82. [PubMed]
  • Croce CM, Girardi AJ, Koprowski H. Assignment of the T-antigen gene of simian virus 40 to human chromosome C-7. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3617–3620. [PMC free article] [PubMed]
  • Solter D, Schachner M. Brain and sperm cell surface antigen (NS-4) on preimplantation mouse embryos. Dev Biol. 1976 Aug;52(1):98–104. [PubMed]
  • Solter D, Knowles BB. Immunosurgery of mouse blastocyst. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5099–5102. [PMC free article] [PubMed]
  • Goldberg EH, Boyse EA, Bennett D, Scheid M, Carswell EA. Serological demonstration of H-Y (male) antigen on mouse sperm. Nature. 1971 Aug 13;232(5311):478–480. [PubMed]
  • Knowles BB, Solter D, Trinchieri G, Maloney KM, Ford SR, Aden DP. Complement-mediated antiserum cytotoxic reactions to human chromosome 7 coded antigen(s): immunoselection of rearranged human chromosome 7 in human-mouse somatic cell hybrids. J Exp Med. 1977 Feb 1;145(2):314–326. [PMC free article] [PubMed]
  • Strickland S, Reich E, Sherman MI. Plasminogen activator in early embryogenesis: enzyme production by trophoblast and parietal endoderm. Cell. 1976 Oct;9(2):231–240. [PubMed]
  • Artzt K, Bennett D, Jacob F. Primitive teratocarcinoma cells express a differentiation antigen specified by a gene at the T-locus in the mouse. Proc Natl Acad Sci U S A. 1974 Mar;71(3):811–814. [PMC free article] [PubMed]
  • Stern PL, Willison KR, Lennox E, Galfrè G, Milstein C, Secher D, Ziegler A. Monoclonal antibodies as probes for differentiation and tumor-associated antigens: a Forssman specificity on teratocarcinoma stem cells. Cell. 1978 Aug;14(4):775–783. [PubMed]
  • Willison KR, Stern PL. Expression of a Forssman antigenic specificity in the preimplantation mouse embryo. Cell. 1978 Aug;14(4):785–793. [PubMed]

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