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Proc Natl Acad Sci U S A. 1985 Oct; 82(20): 6960–6964.
PMCID: PMC391289

Interspecific chloroplast recombination in a Nicotiana somatic hybrid


Genetic recombination between chloroplasts of two flowering plant species, Nicotiana tabacum and Nicotiana plumbaginifolia, after somatic cell fusion is described. The parental lines differed in three cytoplasmic genetic markers. The N. tabacum mutant SR1-A15 was streptomycin-resistant, defective in chloroplast greening, and lincomycin-sensitive. The N. plumbaginifolia mutant LR400 was streptomycin-sensitive, normal green, and lincomycin-resistant. Streptomycin-resistant clones in cell culture are identified by their ability to form a green callus on a selective medium. Streptomycin resistance in the SR1-A15 mutant could not be expressed due to defective chloroplasts. Protoplasts of the two species were fused, and calli grown from the fused population were screened for the expression of streptomycin resistance from the SR1-A15 line as the result of interspecific chloroplast recombination. A somatic hybrid, pt14, expressed a new combination of the cytoplasmic genetic markers. In the pt14 chloroplast genome three N. tabacum and four N. plumbaginifolia parent specific restriction sites have been identified, indicating that the pt14 chloroplast genome contains at least six recombination sites.

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  • Lemieux C, Turmel M, Seligy VL, Lee RW. Chloroplast DNA recombination in interspecific hybrids of Chlamydomonas: Linkage between a nonmendelian locus for streptomycin resistance and restriction fragments coding for 16S rRNA. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1164–1168. [PMC free article] [PubMed]
  • Chen K, Wildman SG, Smith HH. Chloroplast DNA distribution in parasexual hybrids as shown by polypeptide composition of fraction I protein. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5109–5112. [PMC free article] [PubMed]
  • Galun E, Arzee-Gonen P, Fluhr R, Edelman M, Aviv D. Cytoplasmic hybridization in Nicotiana: mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions. Mol Gen Genet. 1982;186(1):50–56. [PubMed]
  • Maliga P, Sz-Breznovits A, Marton L Joo F. Non-Mendelian streptomycin-resistant tobacco mutant with altered chlorplasts and mitochondria. Nature. 1975 May 29;255(5507):401–402. [PubMed]
  • Menczel L, Galiba G, Nagy F, Maliga P. Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana. Genetics. 1982 Mar;100(3):487–495. [PMC free article] [PubMed]
  • Kolodner R, Tewari KK. The molecular size and conformation of the chloroplast DNA from higher plants. Biochim Biophys Acta. 1975 Sep 1;402(3):372–390. [PubMed]
  • Fluhr R, Fromm H, Edelman M. Clone bank of Nicotiana tabacum chloroplast DNA: mapping of the alpha, beta and epsilon subunits of the ATPase coupling factor, the large subunit of ribulosebisphosphate carboxylase, and the 32-kDal membrane protein. Gene. 1983 Nov;25(2-3):271–280. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences


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