• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Jul 1971; 68(7): 1639–1643.
PMCID: PMC389258

Role of Exonuclease and β Protein of Phage λ in Genetic Recombination, V. Recombination of λ DNA in Vitro

Abstract

The sequential action of λ exonuclease and polynucleotide ligase upon redundant joint molecules is sufficient to produce intact polynucleotide chains and heat-stable, biologically active molecules of λ DNA, whereas the action of ligase alone is insufficient. These results (a) confirm the previously described mechanism of single-strand assimilation, including a subsidiary mechanism by which the further action of λ exonuclease is arrested when a redundant strand is completely assimilated, and (b) represent a simulation of the steps in genetic recombination that follow the formation of biparental complexes (synapsis). λ exonuclease is postulated to catalyze a concerted reaction that includes exposure of complementary sequences, formation of heteroduplex regions, and elimination of redundant branches.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (975K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Carter DM, Radding CM. The role of exonuclease and beta protein of phage lambda in genetic recombination. II. Substrate specificity and the mode of action of lambda exonuclease. J Biol Chem. 1971 Apr 25;246(8):2502–2512. [PubMed]
  • Radding CM, Carter DM. The role of exonuclease and beta protein of phage lambda in genetic recombination. 3. Binding to deoxyribonucleic acid. J Biol Chem. 1971 Apr 25;246(8):2513–2518. [PubMed]
  • Sedat JW, Kelly RB, Sinsheimer RL. Fractionation of nucleic acid on benzoylated-naphthoylated DEAE cellulose. J Mol Biol. 1967 Jun 28;26(3):537–540. [PubMed]
  • Mackinlay AG, Kaiser AD. DNA replication in head mutants of bacteriophage lambda. J Mol Biol. 1969 Feb 14;39(3):679–683. [PubMed]
  • Young ET, 2nd, Sinsheimer RL. Vegetative bacteriophage lambda-DNA. I. Infectivity in a spheroplast assay. J Mol Biol. 1967 Nov 28;30(1):147–164. [PubMed]
  • FRASER D, JERREL EA. The amino acid composition of T3 bacteriophage. J Biol Chem. 1953 Nov;205(1):291–295. [PubMed]
  • Iyer VN, Rupp WD. Usefulness of benzoylated naphthoylated DEAE-cellulose to distinguish and fractionate double-stranded DNA bearing different extents of single-stranded regions. Biochim Biophys Acta. 1971 Jan 1;228(1):117–126. [PubMed]
  • Weiss B, Thompson A, Richardson CC. Ezymatic breakage and joining of deoxyribonucleic acid. VII. Properties of the enzyme-adenylate intermediate in the polynucleotide ligase reaction. J Biol Chem. 1968 Sep 10;243(17):4556–4563. [PubMed]
  • Masamune Y, Fleischman RA, Richardson CC. Enzymatic removal and replacement of nucleotides at single strand breaks in deoxyribonucleic acid. J Biol Chem. 1971 Apr 25;246(8):2680–2691. [PubMed]
  • Little JW. An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction. J Biol Chem. 1967 Feb 25;242(4):679–686. [PubMed]
  • Lee CS, Davis RW, Davidson N. A physical study by electron microscopy of the terminally reptitious, circularly permuted DNA from the coliphage particles of Escherichia coli 15. J Mol Biol. 1970 Feb 28;48(1):1–22. [PubMed]
  • Boon T, Zinder ND. A mechanism for genetic recombination generating one parent and one recombinant. Proc Natl Acad Sci U S A. 1969 Oct;64(2):573–577. [PMC free article] [PubMed]
  • Alberts BM, Frey L. T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA. Nature. 1970 Sep 26;227(5265):1313–1318. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...