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Effects of sample handling on plasma ascorbate and dehydroascorbic acid concentrations. Vacutainers with blood samples were prepared under oxidation controlled, standard and exposed conditions as described in Materials and Methods. Plasma total ascorbate (a) and reduced ascorbate (b) levels are highest in controlled samples, showing declines under standard and exposed preparations that are reflected in calculated dehydroascorbic acid levels (c). Significant changes were observed in the exposed group when compared to controlled or standard samples as determined by ANOVA, and denoted with an asterisk (*).

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