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Proc Natl Acad Sci U S A. Dec 1986; 83(23): 9154–9158.
PMCID: PMC387093

Isolation of CD1 genes: a family of major histocompatibility complex-related differentiation antigens.

Abstract

CD1 differentiation antigens are defined by a group of monoclonal antibodies that characterize immature human thymocytes. A cloned cDNA has been used to identify CD1 genes in a human genomic library. Five CD1 genes have been isolated, and Southern blot analysis suggests that these represent all the cross-hybridizing human CD1 genes. They share a highly conserved exon, which is homologous to the beta 2-microglobulin-binding domain (alpha 3) of major histocompatibility complex (MHC) class I antigens. In this domain, amino acid sequences are 71-88% homologous. However, the homology between CD1 and MHC class I alpha 3 domains is only 21%. This is the same degree of homology as between either of them and the class II beta 2 domain, which does not bind beta 2-microglobulin. The evolutionary implications of these results are discussed.

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Selected References

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  • Williams AF, Galfrè G, Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977 Nov;12(3):663–673. [PubMed]
  • Reinherz EL, Schlossman SF. The differentiation and function of human T lymphocytes. Cell. 1980 Apr;19(4):821–827. [PubMed]
  • McMichael AJ, Pilch JR, Galfré G, Mason DY, Fabre JW, Milstein C. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur J Immunol. 1979 Mar;9(3):205–210. [PubMed]
  • Reinherz EL, Kung PC, Goldstein G, Levey RH, Schlossman SF. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1588–1592. [PMC free article] [PubMed]
  • Olive D, Dubreuil P, Mawas C. Two distinct TL-like molecular subsets defined by monoclonal antibodies on the surface of human thymocytes with different expression on leukemia lines. Immunogenetics. 1984;20(3):253–264. [PubMed]
  • Kahn-Perles B, Wietzerbin J, Caillol DH, Lemonnier F. Delineation of three subsets of class I human T antigens (HTA) on Molt-4 cells: serologic and regulatory relationship to HLA class I antigens. J Immunol. 1985 Mar;134(3):1759–1765. [PubMed]
  • Amiot M, Bernard A, Raynal B, Knapp W, Deschildre C, Boumsell L. Heterogeneity of the first cluster of differentiation: characterization and epitopic mapping of three CD1 molecules on normal human thymus cells. J Immunol. 1986 Mar 1;136(5):1752–1758. [PubMed]
  • Bradstock KF, Janossy G, Pizzolo G, Hoffbrand AV, McMichael A, Pilch JR, Milstein C, Beverley P, Bollum FJ. Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies. J Natl Cancer Inst. 1980 Jul;65(1):33–42. [PubMed]
  • Fithian E, Kung P, Goldstein G, Rubenfeld M, Fenoglio C, Edelson R. Reactivity of Langerhans cells with hybridoma antibody. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2541–2544. [PMC free article] [PubMed]
  • Ziegler A, Milstein C. A small polypeptide different from beta2-microglobin associated with a human cell surface antigen. Nature. 1979 May 17;279(5710):243–244. [PubMed]
  • Kefford RF, Calabi F, Fearnley IM, Burrone OR, Milstein C. Serum beta 2-microglobulin binds to a T-cell differentiation antigen and increases its expression. Nature. 1984 Apr 12;308(5960):641–642. [PubMed]
  • Bernabeu C, van de Rijn M, Lerch PG, Terhorst CP. Beta 2-microglobulin from serum associates with MHC class I antigens on the surface of cultured cells. Nature. 1984 Apr 12;308(5960):642–645. [PubMed]
  • Calabi F, Milstein C. A novel family of human major histocompatibility complex-related genes not mapping to chromosome 6. Nature. 1986 Oct 9;323(6088):540–543. [PubMed]
  • Lefranc MP, Rabbitts TH. Two tandemly organized human genes encoding the T-cell gamma constant-region sequences show multiple rearrangement in different T-cell types. Nature. 1985 Aug 1;316(6027):464–466. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Kieny MP, Lathe R, Lecocq JP. New versatile cloning and sequencing vectors based on bacteriophage M13. Gene. 1983 Dec;26(1):91–99. [PubMed]
  • Staden R. The current status and portability of our sequence handling software. Nucleic Acids Res. 1986 Jan 10;14(1):217–231. [PMC free article] [PubMed]
  • Dover G. Molecular drive: a cohesive mode of species evolution. Nature. 1982 Sep 9;299(5879):111–117. [PubMed]
  • Smith GP. Unequal crossover and the evolution of multigene families. Cold Spring Harb Symp Quant Biol. 1974;38:507–513. [PubMed]

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