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Proc Natl Acad Sci U S A. Oct 1986; 83(20): 7821–7825.
PMCID: PMC386814

Toward a physical map of the genome of the nematode Caenorhabditis elegans

Abstract

A technique for digital characterization and comparison of DNA fragments, using restriction enzymes, is described. The technique is being applied to fragments from the nematode Caenorhabditis elegans (i) to facilitate cross-indexing of clones emanating from different laboratories and (ii) to construct a physical map of the genome. Eight hundred sixty clusters of clones, from 35 to 350 kilobases long and totaling about 60% of the genome, have been characterized.

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Selected References

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  • Olson MV, Dutchik JE, Graham MY, Brodeur GM, Helms C, Frank M, MacCollin M, Scheinman R, Frank T. Random-clone strategy for genomic restriction mapping in yeast. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7826–7830. [PMC free article] [PubMed]
  • Sulston JE, Brenner S. The DNA of Caenorhabditis elegans. Genetics. 1974 May;77(1):95–104. [PMC free article] [PubMed]
  • Albertson DG. Mapping muscle protein genes by in situ hybridization using biotin-labeled probes. EMBO J. 1985 Oct;4(10):2493–2498. [PMC free article] [PubMed]
  • Cami B, Kourilsky P. Screening of cloned recombinant DNA in bacteria by in situ colony hybridization. Nucleic Acids Res. 1978 Jul;5(7):2381–2390. [PMC free article] [PubMed]
  • Murray NE, Brammar WJ, Murray K. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977 Jan 7;150(1):53–61. [PubMed]
  • Karn J, Matthes HW, Gait MJ, Brenner S. A new selective phage cloning vector, lambda 2001, with sites for XbaI, BamHI, HindIII, EcoRI, SstI and XhoI. Gene. 1984 Dec;32(1-2):217–224. [PubMed]
  • Ish-Horowicz D, Burke JF. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. [PMC free article] [PubMed]
  • Little PF, Cross SH. A cosmid vector that facilitates restriction enzyme mapping. Proc Natl Acad Sci U S A. 1985 May;82(10):3159–3163. [PMC free article] [PubMed]
  • Sternberg N, Tiemeier D, Enquist L. In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1. Gene. 1977 May;1(3-4):255–280. [PubMed]
  • Gergen JP, Stern RH, Wensink PC. Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res. 1979 Dec 20;7(8):2115–2136. [PMC free article] [PubMed]
  • Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. [PMC free article] [PubMed]
  • Peacock AC, Dingman CW. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. [PubMed]
  • Sanger F, Coulson AR. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. [PubMed]
  • Garoff H, Ansorge W. Improvements of DNA sequencing gels. Anal Biochem. 1981 Aug;115(2):450–457. [PubMed]
  • Blumenthal T, Squire M, Kirtland S, Cane J, Donegan M, Spieth J, Sharrock W. Cloning of a yolk protein gene family from Caenorhabditis elegans. J Mol Biol. 1984 Mar 25;174(1):1–18. [PubMed]
  • Cox GN, Carr S, Kramer JM, Hirsh D. Genetic mapping of Caenorhabditis elegans collagen genes using DNA polymorphisms as phenotypic markers. Genetics. 1985 Mar;109(3):513–528. [PMC free article] [PubMed]
  • Klass MR, Kinsley S, Lopez LC. Isolation and characterization of a sperm-specific gene family in the nematode Caenorhabditis elegans. Mol Cell Biol. 1984 Mar;4(3):529–537. [PMC free article] [PubMed]
  • Staden R. A new computer method for the storage and manipulation of DNA gel reading data. Nucleic Acids Res. 1980 Aug 25;8(16):3673–3694. [PMC free article] [PubMed]
  • Files JG, Hirsh D. Ribosomal DNA of Caenorhabditis elegans. J Mol Biol. 1981 Jun 25;149(2):223–240. [PubMed]
  • Ellis RE, Sulston JE, Coulson AR. The rDNA of C. elegans: sequence and structure. Nucleic Acids Res. 1986 Mar 11;14(5):2345–2364. [PMC free article] [PubMed]
  • Rosenberg SM. EcoK restriction during in vitro packaging of coliphage lambda DNA. Gene. 1985;39(2-3):313–315. [PubMed]

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