• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of pnasPNASInfo for AuthorsSubscriptionsAboutThis Article
Proc Natl Acad Sci U S A. Sep 1986; 83(18): 6692–6696.
PMCID: PMC386575

Isolation, properties, and complete amino acid sequence of human secretory leukocyte protease inhibitor, a potent inhibitor of leukocyte elastase.

Abstract

A potent inhibitor of human leukocyte elastase (EC 3.4.21.37) and cathepsin G (EC 3.4.21.20) and of human trypsin (EC 3.4.21.4) has been purified from human parotid secretions. The complete amino acid sequence of this protein has been determined. The sequence suggests that the protein has two domains of about 54 amino acids, each of which contains four disulfide bonds. On the basis of a limited homology to other protease inhibitors, the antielastase and antitrypsin activities are thought to be properties of the C-terminal and N-terminal domains, respectively. The affinity of the inhibitor for leukocyte elastase is very high, suggesting a functional role for the protein in preventing elastase-mediated damage to oral and possibly other mucosal tissues.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1013K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ohlsson K. Neutral leucocyte proteases and elastase inhibited by plasma alpha 1 -antitrypsin. Scand J Clin Lab Invest. 1971 Nov;28(3):251–253. [PubMed]
  • Klasen EC, Kramps JA. The N-terminal sequence of antileukoprotease isolated from bronchial secretion. Biochem Biophys Res Commun. 1985 Apr 16;128(1):285–289. [PubMed]
  • Smith CE, Johnson DA. Human bronchial leucocyte proteinase inhibitor. Rapid isolation and kinetic analysis with human leucocyte proteinases. Biochem J. 1985 Jan 15;225(2):463–472. [PMC free article] [PubMed]
  • Wallner O, Fritz H. Characterization of an acid-stable proteinase inhibitor in human cervical mucus. Hoppe Seylers Z Physiol Chem. 1974 Jun;355(6):709–715. [PubMed]
  • Hochstrasser K, Haendle H, Reichert R, Werle E. Uber Vorkommen und Eigenschaften eines Proteaseninhibitors in menschlichem Nasensekret. Hoppe Seylers Z Physiol Chem. 1971 Jul;352(7):954–958. [PubMed]
  • Ohlsson M, Fryksmark U, Polling A, Tegner H, Ohlsson K. Localization of antileukoprotease in the parotid and the submandibular salivary glands. Acta Otolaryngol. 1984 Jul-Aug;98(1-2):147–151. [PubMed]
  • Ohlsson M, Rosengren M, Tegner H, Ohlsson K. Quantification of granulocyte elastase inhibitors in human mixed saliva and in pure parotid secretion. Hoppe Seylers Z Physiol Chem. 1983 Sep;364(9):1323–1328. [PubMed]
  • Ohlsson K, Olsson I. The neutral proteases of human granulocytes. Isolation and partial characterization of granulocyte elastases. Eur J Biochem. 1974 Mar 1;42(2):519–527. [PubMed]
  • Ohlsson K, Olsson AS. Immunoreactive granulocyte elastase in human serum. Hoppe Seylers Z Physiol Chem. 1978 Nov;359(11):1531–1539. [PubMed]
  • Mancini G, Carbonara AO, Heremans JF. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. [PubMed]
  • Ohlsson K, Tegner H, Akesson U. Isolation and partial characterization of a low molecular weight acid stable protease inhibitor from human bronchial secretion. Hoppe Seylers Z Physiol Chem. 1977 May;358(5):583–589. [PubMed]
  • Koop DR, Morgan ET, Tarr GE, Coon MJ. Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits. J Biol Chem. 1982 Jul 25;257(14):8472–8480. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Blobel G, Dobberstein B. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol. 1975 Dec;67(3):835–851. [PMC free article] [PubMed]
  • Burnette WN. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981 Apr;112(2):195–203. [PubMed]
  • Glynn P, Gilbert H, Newcombe J, Cuzner ML. Rapid analysis of immunoglobulin isoelectric focusing patterns with cellulose nitrate sheets and immunoperoxidase staining. J Immunol Methods. 1982 Jun 11;51(2):251–257. [PubMed]
  • Jekel PA, Weijer WJ, Beintema JJ. Use of endoproteinase Lys-C from Lysobacter enzymogenes in protein sequence analysis. Anal Biochem. 1983 Oct 15;134(2):347–354. [PubMed]
  • Schenkein I, Levy M, Franklin EC, Frangione B. Proteolytic enzymes from the mouse submaxillary gland. Specificity restricted to arginine residues. Arch Biochem Biophys. 1977 Jul;182(1):64–70. [PubMed]
  • Houmard J, Drapeau GR. Staphylococcal protease: a proteolytic enzyme specific for glutamoyl bonds. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3506–3509. [PMC free article] [PubMed]
  • Laskowski M, Jr, Kato I. Protein inhibitors of proteinases. Annu Rev Biochem. 1980;49:593–626. [PubMed]
  • Seemüller U, Arnhold M, Fritz H, Wiedenmann K, Machleidt W, Heinzel R, Appelhans H, Gassen HG, Lottspeich F. The acid-stable proteinase inhibitor of human mucous secretions (HUSI-I, antileukoprotease). Complete amino acid sequence as revealed by protein and cDNA sequencing and structural homology to whey proteins and Red Sea turtle proteinase inhibitor. FEBS Lett. 1986 Apr 7;199(1):43–48. [PubMed]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Gene
    Gene
    Gene links
  • Gene (nucleotide)
    Gene (nucleotide)
    Records in Gene identified from shared sequence links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Nucleotide
    Nucleotide
    Published Nucleotide sequences
  • OMIM
    OMIM
    OMIM record citing PubMed
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...