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Proc Natl Acad Sci U S A. Sep 1983; 80(18): 5704–5708.
PMCID: PMC384327

RNA transcripts for I-J polypeptides are apparently not encoded between the I-A and I-E subregions of the murine major histocompatibility complex.

Abstract

The I-J subregion of the mouse major histocompatibility complex has been reported to encode antigenic determinants expressed by suppressor T cells. Previously, cosmid clones were obtained from mouse sperm DNA that contain all of the sequences between the I-A and I-E subregions, where I-J has been mapped genetically. However, hybridization of these sequences to RNA prepared from several I-J-positive suppressor T-cell hybridomas did not reveal the presence of a transcript. In addition, no rearrangements in this DNA were detected in the suppressor T cells that we have analyzed. Our results indicate that the I-J polypeptides are not encoded between the I-A and I-E subregions of the major histocompatibility complex. We discuss several hypotheses concerning the possible location and expression of I-J genes.

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Selected References

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  • Klein J, Juretic A, Baxevanis CN, Nagy ZA. The traditional and a new version of the mouse H-2 complex. Nature. 1981 Jun 11;291(5815):455–460. [PubMed]
  • Shreffler DC, David CS, Cullen SE, Frelinger JA, Niederhuber JE. Serological and functional evidence for further subdivision of the I regions of the H-2 gene complex. Cold Spring Harb Symp Quant Biol. 1977;41(Pt 2):477–487. [PubMed]
  • Hauptfeld V, Hauptfeld M, Klein J. Tissue distribution of I region-associated antigens in the mouse. J Immunol. 1974 Jul;113(1):181–188. [PubMed]
  • Thomas DW, Yamashita U, Shevach EM. Nature of the antigenic complex recognized by T lymphocytes. IV. Inhibition of antigen-specific T cell proliferation by antibodies to stimulator macrophage Ia antigens. J Immunol. 1977 Jul;119(1):223–226. [PubMed]
  • Swierkosz JE, Marrack P, Kappler JW. The role of H-2-linked genes in helper T cell function. V. I-region control of helper T cell interaction with antigen-presenting macrophages. J Immunol. 1979 Aug;123(2):654–659. [PubMed]
  • Baxevanis CN, Nagy ZA, Klein J. A novel type of T-T cell interaction removes the requirement for I-B region in the H-2 complex. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3809–3813. [PMC free article] [PubMed]
  • Berzofsky JA, Pisetsky DS, Killion DJ, Hicks G, Sachs DH. Ir genes of different high responder haplotypes for staphylococcal nuclease are not allelic. J Immunol. 1981 Dec;127(6):2453–2455. [PubMed]
  • Waltenbaugh C. Regulation of immune responses by I-J gene products. I. Production and characterization of anti-I-J monoclonal antibodies. J Exp Med. 1981 Nov 1;154(5):1570–1583. [PMC free article] [PubMed]
  • Kanno M, Kobayashi S, Tokuhisa T, Takei I, Shinohara N, Taniguchi M. Monoclonal antibodies that recognize the product controlled by a gene in the I-J subregion of the mouse H-2 complex. J Exp Med. 1981 Nov 1;154(5):1290–1304. [PMC free article] [PubMed]
  • Murphy DB, Herzenberg LA, Okumura K, Herzenberg LA, McDevitt HO. A new I subregion (I-J) marked by a locus (Ia-4) controlling surface determinants on suppressor T lymphocytes. J Exp Med. 1976 Sep 1;144(3):699–712. [PMC free article] [PubMed]
  • Tada T, Taniguchi M, David CS. Properties of the antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. IV. Special subregion assignment of the gene(s) that codes for the suppressive T-cell factor in the H-2 histocompatibility complex. J Exp Med. 1976 Sep 1;144(3):713–725. [PMC free article] [PubMed]
  • Tada T, Takemori T, Okumura K, Nonaka M, Tokuhisa T. Two distinct types of helper T cells involved in the secondary antibody response: independent and synergistic effects of Ia- and Ia+ helper T cells. J Exp Med. 1978 Feb 1;147(2):446–458. [PMC free article] [PubMed]
  • Niederhuber JE, Allen P, Mayo L. The expression of Ia antigenic determinants on macrophages required for the in vitro antibody response. J Immunol. 1979 Apr;122(4):1342–1349. [PubMed]
  • Taniguchi M, Tokuhisa T, Kanno M, Yaoita Y, Shimizu A, Honjo T. Reconstitution of antigen-specific suppressor activity with translation products of mRNA. Nature. 1982 Jul 8;298(5870):172–174. [PubMed]
  • Taniguchi M, Takei I, Tada T. Functional and molecular organisation of an antigen-specific suppressor factor from a T-cell hybridoma. Nature. 1980 Jan 10;283(5743):227–228. [PubMed]
  • Krupen K, Araneo BA, Brink L, Kapp JA, Stein S, Wieder KJ, Webb DR. Purification and characterization of a monoclonal T-cell suppressor factor specific for poly(LGlu60LAla30LTyr10). Proc Natl Acad Sci U S A. 1982 Feb;79(4):1254–1258. [PMC free article] [PubMed]
  • Wieder KJ, Araneo BA, Kapp JA, Webb DR. Cell-free translation of a biologically active, antigen-specific suppressor T cell factor. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3599–3603. [PMC free article] [PubMed]
  • Steinmetz M, Minard K, Horvath S, McNicholas J, Srelinger J, Wake C, Long E, Mach B, Hood L. A molecular map of the immune response region from the major histocompatibility complex of the mouse. Nature. 1982 Nov 4;300(5887):35–42. [PubMed]
  • Trial J, Kapp JA, Pierce CW, Shreffler DC, Sorensen CM, Waltenbaugh C. Expression of cell surface antigens by suppressor T cell hybridomas. I. Comparison of phenotype and function. J Immunol. 1983 Feb;130(2):565–572. [PubMed]
  • Ochi A, Nonaka M, Hayakawa K, Okumura K, Tada T. Two loci in I-J subregion of the H-2 complex controlling molecules selectively expressed on suppressor and helper T cells. J Immunol. 1982 Jul;129(1):227–231. [PubMed]
  • Kronenberg M, Davis MM, Early PW, Hood LE, Watson JD. Helper and killer T cells do not express B cell immunoglobulin joining and constant region gene segments. J Exp Med. 1980 Dec 1;152(6):1745–1761. [PMC free article] [PubMed]
  • Kraig E, Kronenberg M, Kapp JA, Pierce CW, Abruzzini AF, Sorensen CM, Samelson LE, Schwartz RH, Hood LE. T and B cells that recognize the same antigen do not transcribe similar heavy chain variable region gene segments. J Exp Med. 1983 Jul 1;158(1):192–209. [PMC free article] [PubMed]
  • Steinmetz M, Frelinger JG, Fisher D, Hunkapiller T, Pereira D, Weissman SM, Uehara H, Nathenson S, Hood L. Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes. Cell. 1981 Apr;24(1):125–134. [PubMed]
  • Sorensen CM, Pierce CW. Antigen-specific suppression in genetic responder mice to L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT). Characterization of conventional and hybridoma-derived factors produced by suppressor T cells from mice injected as neonates with syngeneic GAT macrophages. J Exp Med. 1982 Dec 1;156(6):1691–1710. [PMC free article] [PubMed]
  • Korman AJ, Knudsen PJ, Kaufman JF, Strominger JL. cDNA clones for the heavy chain of HLA-DR antigens obtained after immunopurification of polysomes by monoclonal antibody. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1844–1848. [PMC free article] [PubMed]
  • Wake CT, Long EO, Strubin M, Gross N, Accolla R, Carrel S, Mach B. Isolation of cDNA clones encoding HLA-DR alpha chains. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6979–6983. [PMC free article] [PubMed]
  • Wiman K, Larhammar D, Claesson L, Gustafsson K, Schenning L, Bill P, Böhme J, Denaro M, Dobberstein B, Hammerling U, et al. Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1703–1707. [PMC free article] [PubMed]
  • Kronenberg M, Kraig E, Siu G, Kapp JA, Kappler J, Marrack P, Pierce CW, Hood L. Three T cell hybridomas do not contain detectable heavy chain variable gene transcripts. J Exp Med. 1983 Jul 1;158(1):210–227. [PMC free article] [PubMed]
  • Jones PP, Murphy DB, McDevitt HO. Variable synthesis and expression of E alpha and Ae (E beta) Ia polypeptide chains in mice of different H-2 haplotypes. Immunogenetics. 1981;12(3-4):321–337. [PubMed]
  • Cook RG, Vitetta ES, Uhr JW, Capra JD. Structural studies on the murine Ia alloantigens. V. Evidence that the structural gene for the I-E/C beta polypeptide is encoded within the I-A subregion. J Exp Med. 1979 Apr 1;149(4):981–986. [PMC free article] [PubMed]

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