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Proc Natl Acad Sci U S A. Jun 1979; 76(6): 2996–3000.
PMCID: PMC383738

Amino-terminal sequence of bovine leukemia virus major internal protein: homology with mammalian type C virus p30 structural proteins.

Abstract

The amino acid composition, the COOH-terminal amino acid, and the NH2-terminal amino acid sequence of the first 55 residues of the major internal structural protein, p24, of bovine leukemia virus (BLV) were determined. The compositional data and the results of end-group analysis revealed that, although BLV p24 is chemically distinct, it more closely resembles the p30 structural proteins than the other gag gene products of mammalian retroviruses. It was found that BLV p24 shares the common NH2-terminal proline and COOH-terminal leucine but lacks the common prolylleucylarginine tripeptide and the larger conserved region found near the NH2 terminus of all mammalian type C viral p30s. Alignment of the amino acid sequence of BLV p24 with the previously determined sequence of feline leukemia virus p27 revealed a statistically significant sequence homology. A more distant relationship was found between BLV p24 and other mammalian p30s. The finding of a definite sequence homology between BLV p24 and mammalian type C virus p30s clearly establishes the origin of these contemporary viral proteins from common progenitor genes.

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  • CROSHAW JE, Jr, ABT DA, MARSHAK RR, HARE WC, SWITZER J, IPSEN J, DUTCHER RM. PEDIGREE STUDIES IN BOVINE LYMPHOSARCOMA. Ann N Y Acad Sci. 1963 Nov 4;108:1193–1202. [PubMed]
  • Miller JM, Miller LD, Olson C, Gillette KG. Virus-like particles in phytohemagglutinin-stimulated lymphocyte cultures with reference to bovine lymphosarcoma. J Natl Cancer Inst. 1969 Dec;43(6):1297–1305. [PubMed]
  • Miller JM, Olson C. Precipitating antibody to an internal antigen of the C-type virus associated with bovine lymphosarcoma. J Natl Cancer Inst. 1972 Nov;49(5):1459–1462. [PubMed]
  • Van Der Maaten MJ, Miller JM, Boothe AD. Replicating type-C virus particles in monolayer cell cultures of tissues from cattle with lymphosarcoma. J Natl Cancer Inst. 1974 Feb;52(2):491–497. [PubMed]
  • Ferrer JF, Abt DA, Bhatt DM, Marshak RR. Studies on the relationship between infection with bovine C-type virus, leukemia, and persistent lymphocytosis in cattle. Cancer Res. 1974 Apr;34(4):893–900. [PubMed]
  • Piper CE, Abt DA, Ferrer JF, Marshak RR. Seroepidemiological evidence for horizontal transmission of bovine C-type virus. Cancer Res. 1975 Oct;35(10):2714–2716. [PubMed]
  • Devare SG, Stephenson JR, Sarma PS, Aaronson SA, Charder S. Bovine lymphosarcoma: development of a radioimmunologic technique for detection of the etiologic agent. Science. 1976 Dec 24;194(4272):1428–1430. [PubMed]
  • Levy D, Deshayes L, Parodi AL, Levy JP, Stephenson JR, Devare SG, Gilden RV. Bovine leukemia virus specific antibodies among French cattle. II. Radioimmunoassay with the major structural protein (BLV p24). Int J Cancer. 1977 Oct 15;20(4):543–550. [PubMed]
  • Mussgay M, Kaaden OR. Progress in studies on the etiology and serologic diagnosis of enzootic bovine leukosis. Curr Top Microbiol Immunol. 1978;79:43–72. [PubMed]
  • Kettmann R, Portetelle D, Mammerickx M, Cleuter Y, Dekegel D, Galoux M, Ghysdael J, Burny A, Chantrenne H. Bovine leukemia virus: an exogenous RNA oncogenic virus. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1014–1018. [PMC free article] [PubMed]
  • Callahan R, Lieber MM, Todaro GJ, Graves DC, Ferrer JF. Bovine leukemia virus genes in the DNA of leukemic cattle. Science. 1976 Jun 4;192(4243):1005–1007. [PubMed]
  • Calafat J, Hageman PC, Ressang AA. Structure of C-type virus particles in lymphocyte cultures of bovine origin. J Natl Cancer Inst. 1974 Apr;52(4):1251–1257. [PubMed]
  • Gilden RV, Long CW, Hanson M, Toni R, Charman HP, Oroszlan S, Miller JM, Van der Maaten MJ. Characteristics of the major internal protein and RNA-dependent DNA polymerase of bovine leukaemia virus. J Gen Virol. 1975 Dec;29(3):305–314. [PubMed]
  • Devare SG, Stephenson JR. Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus. J Virol. 1977 Aug;23(2):443–447. [PMC free article] [PubMed]
  • McDonald HC, Graves DC, Ferrer JF. Isolation and characterization of an antigen of the bovine C-type virus. Cancer Res. 1976 Apr;36(4):1251–1257. [PubMed]
  • Geering G, Hardy WD, Jr, Old LJ, de Harven E, Brodey RS. Shared group-specific antigen of murine and feline leukemia viruses. Virology. 1968 Dec;36(4):678–680. [PubMed]
  • Geering G, Aoki T, Old LJ. Shared viral antigen of mammalian leukaemia viruses. Nature. 1970 Apr 18;226(5242):265–266. [PubMed]
  • Gilden RV, Oroszlan S, Huebner RJ. Coexistence of intraspecies and interspecies specific antigenic determinants on the major structural polypeptide of mammalian C-type viruses. Nat New Biol. 1971 May 26;231(21):107–108. [PubMed]
  • Oroszlan S, Huebner RJ, Gilden RV. Species-specific and interspecific antigenic determinants associated with the structural protein of feline C-type virus. Proc Natl Acad Sci U S A. 1971 May;68(5):901–904. [PMC free article] [PubMed]
  • Parks WP, Scolnick EM. Radioimmunoassay of mammalian type-C viral proteins: interspecies antigenic reactivities of the major internal polypeptide. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1766–1770. [PMC free article] [PubMed]
  • Strand M, August JT. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. J Virol. 1974 Jan;13(1):171–180. [PMC free article] [PubMed]
  • Barbacid M, Stephenson JR, Aaronson SA. Structural polypeptides of mammalian type C RNA viruses. Isolation and immunologic characterization of a low molecular weight polypeptide, p10. J Biol Chem. 1976 Aug 25;251(16):4859–4866. [PubMed]
  • Stephenson JR, Reynolds RK, Devare SG, Reynolds FH. Biochemical and immunological properties of gag genecoded structural proteins of endogenous tyep C RNA tumor viruses of diverse mammalian species. J Biol Chem. 1977 Nov 10;252(21):7818–7825. [PubMed]
  • Oroszlan S, Copeland T, Summers MR, Smythers G, Gilden RV. Amino acid sequence homology of mammalian type C RNA virus major internal proteins. J Biol Chem. 1975 Aug 25;250(16):6232–6239. [PubMed]
  • Oroszlan S, Henderson LE, Stephenson JR, Copeland TD, Long CW, Ihle JN, Gilden RV. Amino- and carboxyl-terminal amino acid sequences of proteins coded by gag gene of murine leukemia virus. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1404–1408. [PMC free article] [PubMed]
  • Boyer SH, Noyes AN, Boyer ML, Marr K. Hemoglobin 3 chains in apes. Primary structures and the presumptive nature of back mutation in a normally silent gene. J Biol Chem. 1973 Feb 10;248(3):992–1003. [PubMed]
  • Henderson LE, Copeland TD, Smythers GW, Marquardt H, Oroszlan S. Amino-terminal amino acid sequence and carboxyl-terminal analysis of Rauscher murine leukemia virus glycoproteins. Virology. 1978 Mar;85(1):319–322. [PubMed]
  • Edman P, Begg G. A protein sequenator. Eur J Biochem. 1967 Mar;1(1):80–91. [PubMed]
  • Tarr GE, Beecher JF, Bell M, McKean DJ. Polyquarternary amines prevent peptide loss from sequenators. Anal Biochem. 1978 Feb;84(2):622–7?0=ENG. [PubMed]
  • Klapper DG, Wilde CE, 3rd, Capra JD. Automated amino acid sequence of small peptides utilizing Polybrene. Anal Biochem. 1978 Mar;85(1):126–131. [PubMed]
  • Hunkapiller MW, Hood LE. Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (polybrene), and high pressure liquid chromatography. Biochemistry. 1978 May 30;17(11):2124–2133. [PubMed]
  • Brauer AW, Margolies MN, Haber E. The application of 0.1 M quadrol to the microsequence of proteins and the sequence of tryptic peptides. Biochemistry. 1975 Jul;14(13):3029–3035. [PubMed]
  • Smithies O, Gibson D, Fanning EM, Goodfliesh RM, Gilman JG, Ballantyne DL. Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac. Biochemistry. 1971 Dec 21;10(26):4912–4921. [PubMed]
  • Capaldi RA, Vanderkooi G. The low polarity of many membrane proteins. Proc Natl Acad Sci U S A. 1972 Apr;69(4):930–932. [PMC free article] [PubMed]
  • Webster RG, Laver WG. Antigenic variation in influenza virus. Biology and chemistry. Prog Med Virol. 1971;13:271–338. [PubMed]

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