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PMC full text:
Cell Rep. Author manuscript; available in PMC 2013 Nov 19.
Published in final edited form as:
Cell Rep. 2013 Oct 31; 5(2): 10.1016/j.celrep.2013.08.047.
Published online 2013 Oct 3. doi:  10.1016/j.celrep.2013.08.047

Figure 5

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ABHD6 Knockdown Results in Modest Alterations in Hepatic Monoacylglycerol Levels, Yet Does Not Alter Hepatic Endocannabinoid Levels or Acute Cannabinoid Receptor 1 (CB1) Signaling in Mouse Liver

(A) Male C57BL/6 mice were fed a chow or high fat diet (HFD) and treated with a control ASO or an ASO targeting ABHD6 for 12 weeks. The hepatic levels of monoacylglycerol (MAG) species were measured by mass spectrometry as described in the methods section. Data represent the mean ± SEM (n = 6), * = P < 0.05 (vs. control ASO within each diet).

(B and C) Male C57BL/6 mice were fed a high fat diet (HFD) and treated with a control non-targeting ASO or an ASO targeting the knockdown of ABHD6 for 12 weeks. The hepatic levels of 2-arachidonylglycerol (B) and anandamide (C) were measured by mass spectrometry as described in the methods section. Data represent the mean ± SEM (n = 6), and no significant differences were found.

(D) Male C57BL/6 mice were fed a high fat diet (HFD) and treated with a control ASO or an ASO targeting ABHD6 for 8 weeks. Following 8 weeks of ASO treatment, mice were fasted for 12 h and subsequently injected with either vehicle or CP-55,940 (0.1 mg/kg) directly into the portal vein. Exactly 5 min later, livers were excised and immediately snap frozen in liquid nitrogen. Protein extracts from the liver were analyzed by Western blotting for ABHD6, phospho-ERK (Thr202/Tyr204), total ERK MAPK, monoacylglycerol lipase (MAGL), or beta actin (β-actin); three representative animals are shown for each group.

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