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Proc Natl Acad Sci U S A. 1996 Oct 29; 93(22): 12142–12149.

The N-end rule: functions, mysteries, uses.


The N-end rule relates the in vivo half-life of a protein to the identity of its N-terminal residue. Similar but distinct versions of the N-end rule operate in all organisms examined, from mammals to fungi and bacteria. In eukaryotes, the N-end rule pathway is a part of the ubiquitin system. I discuss the mechanisms and functions of this pathway, and consider its applications.

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