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Journal List > Appl Microbiol > v.17(4); Apr 1969

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Appl Microbiol. 1969 April; 17(4): 636–638.
PMCID: PMC377759
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Concentration and Purification of Herpesviruses (Simplex, Cytomegalo, and EB) in a Zonal Ultracentrifuge
Robert M. McCombs
Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas 77025
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Abstract
By using the BXIV zonal ultracentrifuge rotor, it was possible to efficiently concentrate and partially purify members of the herpesvirus group by pelleting the virus onto a double sucrose cushion.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
  • Anderson NG, Waters DA, Fisher WD, Cline GB, Nunley CE, Elrod LH, Rankin CT., Jr Analytical techniques for cell fractions. V. Characteristics of the B-XIV and B-XV zonal centrifuge rotors. Anal Biochem. 1967 Nov;21(2):235–252. [PubMed]
  • Benyesh-Melnick M, Phillips CF, Lewis RT, Seidel EH. Studies on acute leukemia and infectious mononucleosis of childhood. IV. Continuous propagation of lymphoblastoid cells from spontaneously transformed bone marrow cultures. J Natl Cancer Inst. 1968 Jan;40(1):123–134. [PubMed]
  • Benyesh-Melnick M, Probstmeyer F, McCombs R, Brunschwig JP, Vonka V. Correlation between infectivity and physical virus particles in human cytomegalovirus. J Bacteriol. 1966 Nov;92(5):1555–1561. [PubMed]
  • Berman AS. Theory of centrifugation: miscellaneous studies. Natl Cancer Inst Monogr. 1966 Jun;21:41–76. [PubMed]
  • Fox SM, Birnie GD, Harvey DR, Martin EM, Sonnabend JA. The use of batch-type zonal ultracentrifuge rotors for the isolation and purification of viruses. J Gen Virol. 1968 May;2(3):455–459. [PubMed]
  • McCombs RM, Melnick MB, Brunschwig JP. Biophysical studies of vesicular stomatitis virus. J Bacteriol. 1966 Feb;91(2):803–812. [PubMed]
  • Vonka V, Benyesh-Melnick M. Interactions of human cytomegalovirus with human fibroblasts. J Bacteriol. 1966 Jan;91(1):213–220. [PubMed]
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