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J Virol. Jun 1970; 5(6): 795–800.
PMCID: PMC376072

Stalked Bacteria: Properties of Deoxriybonucleic Acid Bacteriophage [var phi]CbK

Abstract

The Caulobacter crescentus bacteriophage [var phi]CbK was studied with respect to the physical and chemical properties of both the phage and its deoxyribonucleic acid (DNA). Electron micrographs reveal the phage to be among the largest DNA bacteriophages reported, with head dimensions of 64 by 195 nm and a flexible tail 275 nm in length. The phage is composed of 57% DNA. This DNA is double-stranded as indicated by (i) the sharp increase in extinction coefficient over a narrow range of temperature increase, (ii) an increase in density in CsCl upon thermal denaturation, and (iii) the equivalence of guanine and cytosine as well as adenine and thymine, determined by chemical analysis. [var phi]CbK DNA cosediments with Escherichia coli phage T2 DNA and has therefore been assigned an S20,w value of 63.5S. The size of the phage and its DNA and the percentage of DNA indicate that the [var phi]CbK phage head is relatively loosely packed. The properties of the DNA from bacteriophage [var phi]CbK are similar to those of host C. crescentus DNA with respect to buoyant density, thermal transition point, and guanine plus cytosine content.

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Selected References

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