• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of microrevMicrobiol Mol Biol Rev ArchivePermissionsJournals.ASM.orgMMBR ArticleJournal InfoAuthorsReviewers
Microbiol Rev. Dec 1989; 53(4): 517–530.
PMCID: PMC372751

Genetics and molecular biology of siderophore-mediated iron transport in bacteria.


The possession of specialized iron transport systems may be crucial for bacteria to override the iron limitation imposed by the host or the environment. One of the most commonly found strategies evolved by microorganisms is the production of siderophores, low-molecular-weight iron chelators that have very high constants of association for their complexes with iron. Thus, siderophores act as extracellular solubilizing agents for iron from minerals or organic compounds, such as transferrin and lactoferrin in the host vertebrate, under conditions of iron limitation. Transport of iron into the cell cytosol is mediated by specific membrane receptor and transport systems which recognize the iron-siderophore complexes. In this review I have analyzed in detail three siderophore-mediated iron uptake systems: the plasmid-encoded anguibactin system of Vibrio anguillarum, the aerobactin-mediated iron assimilation system present in the pColV-K30 plasmid and in the chromosomes of many enteric bacteria, and the chromosomally encoded enterobactin iron uptake system, found in Escherichia coli, Shigella spp., Salmonella spp., and other members of the family Enterobacteriaceae. The siderophore systems encoded by Pseudomonas aeruginosa, namely, pyochelin and pyoverdin, as well as the siderophore amonabactin, specified by Aeromonas hydrophila, are also discussed. The potential role of siderophore-mediated systems as virulence determinants in the specific host-bacteria interaction leading to disease is also analyzed with respect to the influence of these systems in the expression of other factors, such as toxins, in the bacterial virulence repertoire.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.7M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Actis LA, Fish W, Crosa JH, Kellerman K, Ellenberger SR, Hauser FM, Sanders-Loehr J. Characterization of anguibactin, a novel siderophore from Vibrio anguillarum 775(pJM1). J Bacteriol. 1986 Jul;167(1):57–65. [PMC free article] [PubMed]
  • Actis LA, Potter SA, Crosa JH. Iron-regulated outer membrane protein OM2 of Vibrio anguillarum is encoded by virulence plasmid pJM1. J Bacteriol. 1985 Feb;161(2):736–742. [PMC free article] [PubMed]
  • Actis LA, Tolmasky ME, Farrell DH, Crosa JH. Genetic and molecular characterization of essential components of the Vibrio anguillarum plasmid-mediated iron-transport system. J Biol Chem. 1988 Feb 25;263(6):2853–2860. [PubMed]
  • Ames GF. Bacterial periplasmic transport systems: structure, mechanism, and evolution. Annu Rev Biochem. 1986;55:397–425. [PubMed]
  • Ankenbauer R, Sriyosachati S, Cox CD. Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin. Infect Immun. 1985 Jul;49(1):132–140. [PMC free article] [PubMed]
  • Ankenbauer RG, Toyokuni T, Staley A, Rinehart KL, Jr, Cox CD. Synthesis and biological activity of pyochelin, a siderophore of Pseudomonas aeruginosa. J Bacteriol. 1988 Nov;170(11):5344–5351. [PMC free article] [PubMed]
  • Arnold RR, Cole MF, McGhee JR. A bactericidal effect for human lactoferrin. Science. 1977 Jul 15;197(4300):263–265. [PubMed]
  • Bagg A, Neilands JB. Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli. Biochemistry. 1987 Aug 25;26(17):5471–5477. [PubMed]
  • Bagg A, Neilands JB. Molecular mechanism of regulation of siderophore-mediated iron assimilation. Microbiol Rev. 1987 Dec;51(4):509–518. [PMC free article] [PubMed]
  • Barghouthi S, Young R, Olson MO, Arceneaux JE, Clem LW, Byers BR. Amonabactin, a novel tryptophan- or phenylalanine-containing phenolate siderophore in Aeromonas hydrophila. J Bacteriol. 1989 Apr;171(4):1811–1816. [PMC free article] [PubMed]
  • Bjorn MJ, Iglewski BH, Ives SK, Sadoff JC, Vasil ML. Effect of iron on yields of exotoxin A in cultures of Pseudomonas aeruginosa PA-103. Infect Immun. 1978 Mar;19(3):785–791. [PMC free article] [PubMed]
  • Bjorn MJ, Sokol PA, Iglewski BH. Influence of iron on yields of extracellular products in Pseudomonas aeruginosa cultures. J Bacteriol. 1979 Apr;138(1):193–200. [PMC free article] [PubMed]
  • Bullen JJ. The significance of iron in infection. Rev Infect Dis. 1981 Nov-Dec;3(6):1127–1138. [PubMed]
  • Bullen JJ, Rogers HJ, Griffiths E. Role of iron in bacterial infection. Curr Top Microbiol Immunol. 1978;80:1–35. [PubMed]
  • Bullen JJ, Rogers HJ, Lewin JE. The bacteriostatic effect of serum on Pasteurella septica and its abolition by iron compounds. Immunology. 1971 Mar;20(3):391–406. [PMC free article] [PubMed]
  • Burkhardt R, Braun V. Nucleotide sequence of the fhuC and fhuD genes involved in iron (III) hydroxamate transport: domains in FhuC homologous to ATP-binding proteins. Mol Gen Genet. 1987 Aug;209(1):49–55. [PubMed]
  • Carlioz A, Ludwig ML, Stallings WC, Fee JA, Steinman HM, Touati D. Iron superoxide dismutase. Nucleotide sequence of the gene from Escherichia coli K12 and correlations with crystal structures. J Biol Chem. 1988 Jan 25;263(3):1555–1562. [PubMed]
  • Casey JL, Hentze MW, Koeller DM, Caughman SW, Rouault TA, Klausner RD, Harford JB. Iron-responsive elements: regulatory RNA sequences that control mRNA levels and translation. Science. 1988 May 13;240(4854):924–928. [PubMed]
  • Cox CD. Iron uptake with ferripyochelin and ferric citrate by Pseudomonas aeruginosa. J Bacteriol. 1980 May;142(2):581–587. [PMC free article] [PubMed]
  • Cox CD. Effect of pyochelin on the virulence of Pseudomonas aeruginosa. Infect Immun. 1982 Apr;36(1):17–23. [PMC free article] [PubMed]
  • Cox CD, Rinehart KL, Jr, Moore ML, Cook JC., Jr Pyochelin: novel structure of an iron-chelating growth promoter for Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4256–4260. [PMC free article] [PubMed]
  • Crichton RR, Charloteaux-Wauters M. Iron transport and storage. Eur J Biochem. 1987 May 4;164(3):485–506. [PubMed]
  • Crosa JH. A plasmid associated with virulence in the marine fish pathogen Vibrio anguillarum specifies an iron-sequestering system. Nature. 1980 Apr 10;284(5756):566–568. [PubMed]
  • Crosa JH. The relationship of plasmid-mediated iron transport and bacterial virulence. Annu Rev Microbiol. 1984;38:69–89. [PubMed]
  • Crosa JH, Hodges LL. Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum 775. Infect Immun. 1981 Jan;31(1):223–227. [PMC free article] [PubMed]
  • Crosa JH, Hodges LL, Schiewe MH. Curing of a plasmid is correlated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum. Infect Immun. 1980 Mar;27(3):897–902. [PMC free article] [PubMed]
  • Crosa LM, Wolf MK, Actis LA, Sanders-Loehr J, Crosa JH. New aerobactin-mediated iron uptake system in a septicemia-causing strain of Enterobacter cloacae. J Bacteriol. 1988 Dec;170(12):5539–5544. [PMC free article] [PubMed]
  • Curtis NA, Eisenstadt RL, East SJ, Cornford RJ, Walker LA, White AJ. Iron-regulated outer membrane proteins of Escherichia coli K-12 and mechanism of action of catechol-substituted cephalosporins. Antimicrob Agents Chemother. 1988 Dec;32(12):1879–1886. [PMC free article] [PubMed]
  • De Lorenzo V, Herrero M, Giovannini F, Neilands JB. Fur (ferric uptake regulation) protein and CAP (catabolite-activator protein) modulate transcription of fur gene in Escherichia coli. Eur J Biochem. 1988 May 2;173(3):537–546. [PubMed]
  • de Lorenzo V, Wee S, Herrero M, Neilands JB. Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor. J Bacteriol. 1987 Jun;169(6):2624–2630. [PMC free article] [PubMed]
  • Eick-Helmerich K, Hantke K, Braun V. Cloning and expression of the exbB gene of Escherichia coli K-12. Mol Gen Genet. 1987 Feb;206(2):246–251. [PubMed]
  • Elish ME, Pierce JR, Earhart CF. Biochemical analysis of spontaneous fepA mutants of Escherichia coli. J Gen Microbiol. 1988 May;134(5):1355–1364. [PubMed]
  • Ernst JF, Bennett RL, Rothfield LI. Constitutive expression of the iron-enterochelin and ferrichrome uptake systems in a mutant strain of Salmonella typhimurium. J Bacteriol. 1978 Sep;135(3):928–934. [PMC free article] [PubMed]
  • Fecker L, Braun V. Cloning and expression of the fhu genes involved in iron(III)-hydroxamate uptake by Escherichia coli. J Bacteriol. 1983 Dec;156(3):1301–1314. [PMC free article] [PubMed]
  • GARIBALDI JA, NEILANDS JB. Formation of iron-binding compounds by micro-organisms. Nature. 1956 Mar 17;177(4507):526–527. [PubMed]
  • Gibson F, Magrath DI. The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I. Biochim Biophys Acta. 1969 Nov 18;192(2):175–184. [PubMed]
  • Griggs DW, Konisky J. Mechanism for iron-regulated transcription of the Escherichia coli cir gene: metal-dependent binding of fur protein to the promoters. J Bacteriol. 1989 Feb;171(2):1048–1054. [PMC free article] [PubMed]
  • Guterman SK. Colicin B: mode of action and inhibition by enterochelin. J Bacteriol. 1973 Jun;114(3):1217–1224. [PMC free article] [PubMed]
  • Hantke K. Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant. Mol Gen Genet. 1981;182(2):288–292. [PubMed]
  • Hantke K, Braun V. Functional interaction of the tonA/tonB receptor system in Escherichia coli. J Bacteriol. 1978 Jul;135(1):190–197. [PMC free article] [PubMed]
  • Hartmann A, Braun V. Iron transport in Escherichia coli: uptake and modification of ferrichrome. J Bacteriol. 1980 Jul;143(1):246–255. [PMC free article] [PubMed]
  • Hentze MW, Caughman SW, Casey JL, Koeller DM, Rouault TA, Harford JB, Klausner RD. A model for the structure and functions of iron-responsive elements. Gene. 1988 Dec 10;72(1-2):201–208. [PubMed]
  • Herrington DA, Sparling PF. Haemophilus influenzae can use human transferrin as a sole source for required iron. Infect Immun. 1985 Apr;48(1):248–251. [PMC free article] [PubMed]
  • Lemos ML, Salinas P, Toranzo AE, Barja JL, Crosa JH. Chromosome-mediated iron uptake system in pathogenic strains of Vibrio anguillarum. J Bacteriol. 1988 Apr;170(4):1920–1925. [PMC free article] [PubMed]
  • Liu J, Duncan K, Walsh CT. Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase. J Bacteriol. 1989 Feb;171(2):791–798. [PMC free article] [PubMed]
  • Luckey M, Pollack JR, Wayne R, Ames BN, Neilands JB. Iron uptake in Salmonella typhimurium: utilization of exogenous siderochromes as iron carriers. J Bacteriol. 1972 Sep;111(3):731–738. [PMC free article] [PubMed]
  • Magazin MD, Moores JC, Leong J. Cloning of the gene coding for the outer membrane receptor protein for ferric pseudobactin, a siderophore from a plant growth-promoting Pseudomonas strain. J Biol Chem. 1986 Jan 15;261(2):795–799. [PubMed]
  • Marolda CL, Valvano MA, Lawlor KM, Payne SM, Crosa JH. Flanking and internal regions of chromosomal genes mediating aerobactin iron uptake systems in enteroinvasive Escherichia coli and Shigella flexneri. J Gen Microbiol. 1987 Aug;133(8):2269–2278. [PubMed]
  • Marugg JD, Nielander HB, Horrevoets AJ, van Megen I, van Genderen I, Weisbeek PJ. Genetic organization and transcriptional analysis of a major gene cluster involved in siderophore biosynthesis in Pseudomonas putida WCS358. J Bacteriol. 1988 Apr;170(4):1812–1819. [PMC free article] [PubMed]
  • Marugg JD, van Spanje M, Hoekstra WP, Schippers B, Weisbeek PJ. Isolation and analysis of genes involved in siderophore biosynthesis in plant-growth-stimulating Pseudomonas putida WCS358. J Bacteriol. 1985 Nov;164(2):563–570. [PMC free article] [PubMed]
  • Matzanke BF, Müller GI, Raymond KN. Hydroxamate siderophore mediated iron uptake in E. coli: stereospecific recognition of ferric rhodotorulic acid. Biochem Biophys Res Commun. 1984 Jun 29;121(3):922–930. [PubMed]
  • Mickelsen PA, Sparling PF. Ability of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species to obtain iron from transferrin and iron compounds. Infect Immun. 1981 Aug;33(2):555–564. [PMC free article] [PubMed]
  • Miles AA, Khimji PL. Enterobacterial chelators of iron: their occurrence, detection, and relation to pathogenicity. J Med Microbiol. 1975 Nov;8(4):477–490. [PubMed]
  • Moores JC, Magazin M, Ditta GS, Leong J. Cloning of genes involved in the biosynthesis of pseudobactin, a high-affinity iron transport agent of a plant growth-promoting Pseudomonas strain. J Bacteriol. 1984 Jan;157(1):53–58. [PMC free article] [PubMed]
  • Nahlik MS, Brickman TJ, Ozenberger BA, McIntosh MA. Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entA. J Bacteriol. 1989 Feb;171(2):784–790. [PMC free article] [PubMed]
  • Neilands JB. Microbial iron compounds. Annu Rev Biochem. 1981;50:715–731. [PubMed]
  • Neilands JB. Iron absorption and transport in microorganisms. Annu Rev Nutr. 1981;1:27–46. [PubMed]
  • Neilands JB. Microbial envelope proteins related to iron. Annu Rev Microbiol. 1982;36:285–309. [PubMed]
  • O'Brien IG, Cox GB, Gibson F. Enterochelin hydrolysis and iron metabolism in Escherichia coli. Biochim Biophys Acta. 1971 Jun 22;237(3):537–549. [PubMed]
  • Ozenberger BA, Brickman TJ, McIntosh MA. Nucleotide sequence of Escherichia coli isochorismate synthetase gene entC and evolutionary relationship of isochorismate synthetase and other chorismate-utilizing enzymes. J Bacteriol. 1989 Feb;171(2):775–783. [PMC free article] [PubMed]
  • Payne SM. Synthesis and utilization of siderophores by Shigella flexneri. J Bacteriol. 1980 Sep;143(3):1420–1424. [PMC free article] [PubMed]
  • Perez-Casal JF, Crosa JH. Aerobactin iron uptake sequences in plasmid ColV-K30 are flanked by inverted IS1-like elements and replication regions. J Bacteriol. 1984 Oct;160(1):256–265. [PMC free article] [PubMed]
  • Pollack JR, Ames BN, Neilands JB. Iron transport in Salmonella typhimurium: mutants blocked in the biosynthesis of enterobactin. J Bacteriol. 1970 Nov;104(2):635–639. [PMC free article] [PubMed]
  • Postle K, Good RF. DNA sequence of the Escherichia coli tonB gene. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5235–5239. [PMC free article] [PubMed]
  • Pressler U, Staudenmaier H, Zimmermann L, Braun V. Genetics of the iron dicitrate transport system of Escherichia coli. J Bacteriol. 1988 Jun;170(6):2716–2724. [PMC free article] [PubMed]
  • Ptashne M. How eukaryotic transcriptional activators work. Nature. 1988 Oct 20;335(6192):683–689. [PubMed]
  • Rao K, Harford JB, Rouault T, McClelland A, Ruddle FH, Klausner RD. Transcriptional regulation by iron of the gene for the transferrin receptor. Mol Cell Biol. 1986 Jan;6(1):236–240. [PMC free article] [PubMed]
  • Russell LM, Holmes RK. Initial characterization of the ferric iron transport system of Corynebacterium diphtheriae. J Bacteriol. 1983 Sep;155(3):1439–1442. [PMC free article] [PubMed]
  • Russell LM, Cryz SJ, Jr, Holmes RK. Genetic and biochemical evidence for a siderophore-dependent iron transport system in Corynebacterium diphtheriae. Infect Immun. 1984 Jul;45(1):143–149. [PMC free article] [PubMed]
  • Salinas PC, Tolmasky ME, Crosa JH. Regulation of the iron uptake system in Vibrio anguillarum: evidence for a cooperative effect between two transcriptional activators. Proc Natl Acad Sci U S A. 1989 May;86(10):3529–3533. [PMC free article] [PubMed]
  • Gross R, Engelbrecht F, Braun V. Identification of the genes and their polypeptide products responsible for aerobactin synthesis by pColV plasmids. Mol Gen Genet. 1985;201(2):204–212. [PubMed]
  • Schmitt MP, Payne SM. Genetics and regulation of enterobactin genes in Shigella flexneri. J Bacteriol. 1988 Dec;170(12):5579–5587. [PMC free article] [PubMed]
  • Sokol PA. Surface expression of ferripyochelin-binding protein is required for virulence of Pseudomonas aeruginosa. Infect Immun. 1987 Sep;55(9):2021–2025. [PMC free article] [PubMed]
  • Sokol PA, Cox CD, Iglewski BH. Pseudomonas aeruginosa mutants altered in their sensitivity to the effect of iron on toxin A or elastase yields. J Bacteriol. 1982 Aug;151(2):783–787. [PMC free article] [PubMed]
  • Sokol PA, Woods DE. Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa. Infect Immun. 1983 May;40(2):665–669. [PMC free article] [PubMed]
  • Sriyosachati S, Cox CD. Siderophore-mediated iron acquisition from transferrin by Pseudomonas aeruginosa. Infect Immun. 1986 Jun;52(3):885–891. [PMC free article] [PubMed]
  • Stoebner JA, Payne SM. Iron-regulated hemolysin production and utilization of heme and hemoglobin by Vibrio cholerae. Infect Immun. 1988 Nov;56(11):2891–2895. [PMC free article] [PubMed]
  • Stuart SJ, Greenwood KT, Luke RK. Hydroxamate-mediated transport of iron controlled by ColV plasmids. J Bacteriol. 1980 Jul;143(1):35–42. [PMC free article] [PubMed]
  • Tai SP, Holmes RK. Iron regulation of the cloned diphtheria toxin promoter in Escherichia coli. Infect Immun. 1988 Sep;56(9):2430–2436. [PMC free article] [PubMed]
  • Takeda Y, Avila H. Structure and gene expression of the E. coli Mn-superoxide dismutase gene. Nucleic Acids Res. 1986 Jun 11;14(11):4577–4589. [PMC free article] [PubMed]
  • Teintze M, Hossain MB, Barnes CL, Leong J, van der Helm D. Structure of ferric pseudobactin, a siderophore from a plant growth promoting Pseudomonas. Biochemistry. 1981 Oct 27;20(22):6446–6457. [PubMed]
  • Tolmasky ME, Actis LA, Crosa JH. Genetic analysis of the iron uptake region of the Vibrio anguillarum plasmid pJM1: molecular cloning of genetic determinants encoding a novel trans activator of siderophore biosynthesis. J Bacteriol. 1988 Apr;170(4):1913–1919. [PMC free article] [PubMed]
  • Tolmasky ME, Crosa JH. Molecular cloning and expression of genetic determinants for the iron uptake system mediated by the Vibrio anguillarum plasmid pJM1. J Bacteriol. 1984 Dec;160(3):860–866. [PMC free article] [PubMed]
  • Tolmasky ME, Salinas PC, Actis LA, Crosa JH. Increased production of the siderophore anguibactin mediated by pJM1-like plasmids in Vibrio anguillarum. Infect Immun. 1988 Jun;56(6):1608–1614. [PMC free article] [PubMed]
  • Toranzo AE, Barja JL, Potter SA, Colwell RR, Hetrick FM, Crosa JH. Molecular factors associated with virulence of marine vibrios isolated from striped bass in Chesapeake Bay. Infect Immun. 1983 Mar;39(3):1220–1227. [PMC free article] [PubMed]
  • Valvano MA, Crosa JH. Aerobactin iron transport genes commonly encoded by certain ColV plasmids occur in the chromosome of a human invasive strain of Escherichia coli K1. Infect Immun. 1984 Oct;46(1):159–167. [PMC free article] [PubMed]
  • Valvano MA, Crosa JH. Molecular cloning, expression, and regulation in Escherichia coli K-12 of a chromosome-mediated aerobactin iron transport system from a human invasive isolate of E. coli K1. J Bacteriol. 1988 Dec;170(12):5529–5538. [PMC free article] [PubMed]
  • Valvano MA, Silver RP, Crosa JH. Occurrence of chromosome- or plasmid-mediated aerobactin iron transport systems and hemolysin production among clonal groups of human invasive strains of Escherichia coli K1. Infect Immun. 1986 Apr;52(1):192–199. [PMC free article] [PubMed]
  • Walter MA, Potter SA, Crosa JH. Iron uptake system medicated by Vibrio anguillarum plasmid pJM1. J Bacteriol. 1983 Nov;156(2):880–887. [PMC free article] [PubMed]
  • Waters VL, Crosa JH. DNA environment of the aerobactin iron uptake system genes in prototypic ColV plasmids. J Bacteriol. 1986 Aug;167(2):647–654. [PMC free article] [PubMed]
  • Waters VL, Crosa JH. Divergence of the aerobactin iron uptake systems encoded by plasmids pColV-K30 in Escherichia coli K-12 and pSMN1 in Aerobacter aerogenes 62-1. J Bacteriol. 1988 Nov;170(11):5153–5160. [PMC free article] [PubMed]
  • Warner PJ, Williams PH, Bindereif A, Neilands JB. ColV plasmid-specific aerobactin synthesis by invasive strains of Escherichia coli. Infect Immun. 1981 Aug;33(2):540–545. [PMC free article] [PubMed]
  • Wayne R, Neilands JB. Evidence for common binding sites for ferrichrome compounds and bacteriophage phi 80 in the cell envelope of Escherichia coli. J Bacteriol. 1975 Feb;121(2):497–503. [PMC free article] [PubMed]
  • Williams PH. Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect Immun. 1979 Dec;26(3):925–932. [PMC free article] [PubMed]
  • Wolf MK, Crosa JH. Evidence for the role of a siderophore in promoting Vibrio anguillarum infections. J Gen Microbiol. 1986 Oct;132(10):2949–2952. [PubMed]
  • Zimmermann L, Angerer A, Braun V. Mechanistically novel iron(III) transport system in Serratia marcescens. J Bacteriol. 1989 Jan;171(1):238–243. [PMC free article] [PubMed]

Articles from Microbiological Reviews are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...