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J Clin Invest. Apr 1979; 63(4): 759–764.
PMCID: PMC372012

Immunoglobulin G independent activation of the classical complement pathway by monosodium urate crystals.

Abstract

10 mg of monosodium urate crystals reduced the CH50 of 1 ml of human serum by 57% after 30 min at 37 degrees C. C1, C4, and C3 depletion of 52, 68, and 46% were typical of classical pathway activation. C1 binding and activation occurred when urate crystals were incubated with isolated precursor C1, and required the intact macromolecule, C1qrs. Activation of isolated C1 by urate crystals was not diminished by F(ab')2 anti-Fc under conditions in which C1 activation by aggregated immunoglobulin (G) was blocked by the F(ab')2 antibody.

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Selected References

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  • Webster ME, Maling HM, Zweig MH, Williams MA, Anderson W., Jr Urate crystal induced inflammation in the rat: evidence for the combined actions of kinins, histamine and components of complement. Immunol Commun. 1972;1(2):185–198. [PubMed]
  • Kellermeyer RW, Naff GB. Chemical mediators of inflammation in acute gouty arthritis. Arthritis Rheum. 1975 Nov-Dec;18(6 Suppl):765–770. [PubMed]
  • Naff GB, Byers PH. Complement as a mediator of inflammation in acute gouty arthritis. I. Studies on the reaction between human serum complement and sodium urate crystals. J Lab Clin Med. 1973 May;81(5):747–760. [PubMed]
  • Byers PH, Ward PA, Kellermeyer RW, Naff GB. Complement as a mediator of inflammation in acute gouty arthritis. II. Biological activities generated from complement by the interaction of serum complement and sodium urate crystals. J Lab Clin Med. 1973 May;81(5):761–769. [PubMed]
  • Kozin F, McCarty DJ. Protein binding to monosodium urate monohydrate, calcium pyrophosphate dihydrate, and silicon dioxide crystals. I. Physical characteristics. J Lab Clin Med. 1977 Jun;89(6):1314–1325. [PubMed]
  • Ginsberg MH, Kozin F, O'Malley M, McCarty DJ. Release of platelet constituents by monosodium urate crystals. J Clin Invest. 1977 Nov;60(5):999–1007. [PMC free article] [PubMed]
  • HOWELL RR, EANES ED, SEEGMILLER JE. X-ray diffraction studies of the tophaceous deposits in gout. Arthritis Rheum. 1963 Apr;6:97–103. [PubMed]
  • Yonemasu K, Stroud RM. Clq: rapid purification method for preparation of monospecific antisera and for biochemical studies. J Immunol. 1971 Feb;106(2):304–313. [PubMed]
  • Ziccardi RJ, Cooper NR. Physicochemical and functional characterization of the C1r subunit of the first complement component. J Immunol. 1976 Feb;116(2):496–503. [PubMed]
  • Valet G, Cooper NR. Isolation and characterization of the proenzyme form of the C1s subunit of the first complement component. J Immunol. 1974 Jan;112(1):339–350. [PubMed]
  • Cooper NR, Ziccardi RJ. Reconstitution of C1 in native proenzyme form and its use in a quantitative C1 activation test. J Immunol. 1977 Nov;119(5):1664–1667. [PubMed]
  • Ginsberg MH, Kozin F. Mechanisms of cellular interaction with monosodium urate crystals. IgG-dependent and IgG-independent platelet stimulation by urate crystals. Arthritis Rheum. 1978 Nov-Dec;21(8):896–903. [PubMed]
  • Weber K, Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed]
  • Cooper NR, Morrison DC. Binding and activation of the first component of human complement by the lipid A region of lipopolysaccharides. J Immunol. 1978 Jun;120(6):1862–1868. [PubMed]
  • Ulevitch RJ, Johnston AR. The modification of biophysical and endotoxic properties of bacterial lipopolysaccharides by serum. J Clin Invest. 1978 Dec;62(6):1313–1324. [PMC free article] [PubMed]
  • McCarty DJ, Jr, Phelps P, Pyenson J. Crystal-induced inflammation in canine joints. I. An experimental model with quantification of the host response. J Exp Med. 1966 Jul 1;124(1):99–114. [PMC free article] [PubMed]
  • Phelps P, McCarty DJ., Jr Crystal-induced inflammation in canine joints. II. Importance of polymorphonuclear leukocytes. J Exp Med. 1966 Jul 1;124(1):115–126. [PMC free article] [PubMed]
  • Goers JW, Ziccardi RJ, Schumaker VN, Glovsky MM. The mechanism of activation of the first component of complement by a univalent hapten-IgG antibody complex. J Immunol. 1977 Jun;118(6):2182–2191. [PubMed]
  • Ratnoff OD, Naff GB. The conversion of C'IS to C'1 esterase by plasmin and trypsin. J Exp Med. 1967 Feb 1;125(2):337–358. [PMC free article] [PubMed]

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