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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Neurosci. Author manuscript; available in PMC Sep 6, 2013.
Published in final edited form as:
PMCID: PMC3699341
NIHMSID: NIHMS453195

Inhibition of a SNARE sensitive pathway in astrocytes attenuates damage following stroke

Abstract

A strong body of research has defined the role of excitotoxic glutamate in animal models of brain ischemia and stroke, however clinical trials of glutamate receptor antagonists have demonstrated their limited capacity to prevent brain damage following ischemia. We propose that astrocyte-neuron signaling represents an important modulatory target that may be useful in mediating damage following stroke. To assess the impact of astrocyte signaling on damage following stroke we have used the astrocyte specific dominant-negative SNARE mouse model (dnSNARE). Recent findings have shown that the astrocytic SNARE signaling pathway can affect neuronal excitability by regulating the surface expression of NMDA receptors. Using focal photothrombosis via the Rose Bengal method, as well as excitotoxic NMDA lesions, we show that dnSNARE animals exhibited a sparing of damaged tissue quantified using Nissl and NeuN staining. At the same time point, animals were also tested in behavioral tasks that probe the functional integrity of stroke or lesion damaged motor and somatosensory areas. We found that dnSNARE mice performed significantly better than littermate controls on rung walk and adhesive dot removal tasks following lesion. Taken together, our results demonstrate the important role of astrocytic signaling under ischemic conditions. Drugs targeting astrocyte signaling have a potential benefit for the outcome of stroke in human patients by limiting the spread of damage.

Keywords: stroke, astrocyte, NMDA, photothrombosis, sensory-motor behaviors

Introduction

The pathophysiology of neuronal damage caused by ischemic injury, has been extensively studied, however most studies have focused solely on neuronal mechanisms (Barreto et al., 2011a). Ischemia is a serious condition in which the cerebral blood supply within the brain is restricted, typically by thrombotic and/or embolitic events. Severe ischemia causes acute neuronal death within minutes in the ischemic core (infarct), whereas secondary, expanding neuronal death occurs in the penumbra (or peri-infarct) within hours (Ginsberg and Pulsinelli, 1994; Hossmann, 1994). Several mechanisms contribute to the extent of damage, including glutamate and Ca2+ toxicity, oxidative stress, inflammation, mitochondrial dysfunction, and acidosis (Barber et al., 2003; Mattiasson et al., 2003; Xiong et al., 2004; Eltzschig and Eckle, 2011). Extensive research has focused on glutamate-mediated excitotoxicity in animal models of brain ischemia and stroke. However clinical trials of glutamate receptor antagonists have demonstrated their limited capacity to prevent brain damage following human ischemia (Ikonomidou and Turski, 2002; Hoyte et al., 2004; Lipton, 2004; Muir, 2006). The lack of positive results from these approaches may relate to the fundamental role of neuronal glutamate in brain signaling (Ikonomidou and Turski, 2002), and indeed failure of many approaches used to prevent damage following stroke could be due to the exclusive focus on neuronal function.

Astrocytes are the predominant glial-cell type in the CNS that modulate neuronal function although there is little insight into how they might impact the consequences of ischemia (Barreto et al., 2011b). Astrocytes are well known to maintain structural integrity and to be tightly coupled to neuronal metabolism via the vasculature (Haydon and Carmignoto, 2006; Attwell et al., 2010). Astrocytes not only play supportive roles in brain function but also play an active role in modulating neuronal function. Astrocytes express a variety of receptors, some of which are able to induce the release of chemical transmitters for interaction and communication with neurons (for review see (Haydon, 2001)). Astrocytes have been shown to affect neuronal excitability via multiple mechanisms (Oliet et al., 2004; Haydon and Carmignoto, 2006; Zorec et al., 2012). In particular, astrocyte signaling has been shown to regulate the surface expression of N-Methyl-D-aspartate (NMDA) receptors thereby regulating the NMDA component of synaptic transmission (Deng et al., 2011).

Astrocytes retain their structural integrity for hours following photothrombosis, exhibit receptor-mediated Ca2+ oscillations in the ischemic region which when selectively buffered can result in protection from damage in a stroke model (Ding et al., 2009). We have used a genetic mouse model that selectively interferes with astrocyte signaling via conditional expression of a dominant-negative SNARE protein under control of the GFAP promoter (dnSNARE; (Halassa et al., 2009)) to further probe the role of these glia in stroke. We demonstrate that dnSNARE mice show reduced lesion volume and improved behavioral performance following photothrombosis and show improved histological outcomes following NMDA lesions. Taken together, our results demonstrate the important role of astrocytic signaling under ischemic conditions, and provide new insights regarding neuron-glia interactions.

Materials Methods

All procedures were in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals, and were approved by the Tufts University Institutional Animal Care and Use Committee.

dnSNARE Mice

The dnSNARE mouse strain (Pascual et al., 2005) has been previously characterized (Fellin et al., 2009; Halassa et al., 2009). Germ-line transmission of the transgenes was detected using PCR to identify all experimental animals. The dnSNARE mice have been backcrossed onto a C57BL6/J genotype for more than 10 generations, and littermates were used as controls in all experiments. To allow normal brain development, dnSNARE animals and their littermates were maintained on a diet containing doxycycline, to suppress transgene expression, until weaning (Fellin et al., 2009; Halassa et al., 2009). EGFP reporter expression has previously been shown to occur selectively in astrocytes, as demonstrated using antibodies to GFAP (astrocyte marker) and NeuN (neuronal cell marker; (Fellin et al., 2009; Halassa et al., 2009)). All mice used in experiments were males ranging from 12- 16 weeks of age.

Photothrombosis

Unilateral ischemic strokes were induced in the sensory-motor cortex using the photothrombotic method (Watson et al., 1985; Brown et al., 2010). Mice were anesthetized with ketamine/xylazine (intraperitoneally100 mg/kg ketamine and 10 mg/kg xylazine), fitted into a stereotaxic frame. To induce stroke, a cranial window was created above the forelimb cortex (A/P=0.0, M/L +1.5; (Tennant et al., 2011)), and this region was illuminated with green epifluorescent light for 12 mins after an injection of 1% Rose Bengal solution (intraperitoneally, 100 mg/kg, in phosphate-buffered saline).

NMDA Lesion

NMDA lesions were based on previously established protocols (Kim et al., 2006). Mice were anesthetized with ketamine/xylazine (intraperitoneally 100 mg/kg ketamine and 10 mg/kg xylazine), and fitted into a stereotaxic frame. A small burr hole was drilled centered on the forelimb area, and 1 μL of a 10 mg/mL NMDA solution (dissolved in sterile artificial Cerebral Spinal fluid (aCSF)) was injected into the lesion site. In the case of the contralateral lesion, the skull above the area was drilled with a burr hole but no injection was performed.

Histology and Immunohostochemistry

Animals were transcardially perfused with a 4% Paraformaldehyde solution. 40 μm thick sections were cut serially, and a subset was reserved for Nissl staining. Remaining sections were stained free floating with anti-GFP (chicken – Upstate (Millipore)), and anti-NeuN (mouse – Chemicon (Millipore)), followed by corresponding secondary antibodies (Invitrogen). Images were taken with a 20× objective on a Nikon confocal microscope, and were quantified using imageJ.

Behavioral Assessments

Rung walk

The rung walk assessment was based on methods described by (Farr et al., 2006). The rung walk apparatus was composed of two Plexiglas walls (69.5 cm × 15 cm). Each wall contained 121 holes 0.20 cm diameter, spaced 0.5 cm apart, and located 1 cm from the bottom edge of the wall. The holes could be filled with 8 cm long metal bars, diameter 0.10 cm. The walls were spaced 5 cm apart to allow for passage of a mouse but prevent it from turning around. The entire apparatus was placed atop two standard mouse housing cages, 17 cm above the ground. The first tub served as a neutral start location and the goal tub was the animal’s home cage.

Adhesive dot removal

This task was based on methods previously described (Schallert and Whishaw, 1984), and requires mice to detect and remove pieces of adhesive paper from their wrists. The distal-radial aspects of forelimbs were cleaned with 70% alcohol and an adhesive sticker was applied to the limb contralateral to the photothrombotic lesion. We then quantified the latency to sticker removal (average over each of four trials) to assess the ability of the mouse to perform sensorimotor tasks.

Results

Expression of dnSNARE in astrocytes reduces the extent of damage following photothrombosis

Pregnant females and offspring were fed doxycycline containing chow to suppress the expression of EGFP and dnSNARE until weaning (Figure 1A-C). Subsequently mice were separated into two groups, one which was fed normal food and the other that was maintained on a diet containing doxycycline. At 9 weeks of age the intensity of EGFP expression in both groups of animals was compared. In the absence of doxycycline the intensity of EGFP fluorescence was 75.8±7.3 a.u compared to 12.2±2.2 a.u. when doxycycline was maintained in the diet of dnSNARE mice (p<0.001). Doxycycline prevented transgene expression, and thus fluorescence intensity was not different from wildtype littermate control mice (13.6±1.3 a.u.), or wildtype littermate control mice fed doxycycline chow (11.5±1.1 a.u.; Figure 1D).

Figure 1
Damage following Rose Bengal photothrombosis is attenuated in mice expressing dnSNARE in astrocytes

Groups of dnSNARE and matched wildtype littermate controls were subjected to unilateral Rose Bengal photothrombosis using stereotaxic coordinates to generate a cranial window above the forelimb sensory-motor area (A/P=0.0, M/L +1.5; (Tennant et al., 2011)). At 24 hours post-lesion mice were sacrificed by transcardial perfusion and serial tissue sections were cut for analysis of infarct volume with Nissl stained sections (Figure 1E,F). Wildtype mice had a mean infarct volume of 1.80±0.22 mm3, which was significantly greater (p=0.019) than mice expressing dnSNARE (0.93±0.17 mm3; Figure 1G), suggesting that expression of dnSNARE in astrocytes may aid in tissue sparing in the photothrombosis model of stroke.

Neurons are spared within the photothrombosis infarct and peri-infarct regions in mice expressing dnSNARE

Tissue sections from mice that had undergone unilateral photothrombosis were also processed for immunohistochemistry for the neuronal marker NeuN, to allow an assessment of neurons in the infarct and peri-infarct regions (Figure 2A,B). To examine the number of NeuN positive cells we sampled multiple regions, 50 μm tall by 50 μm wide and 25 μm deep (62500 μm3). Within the infarct region, wildtype mice had an average of 1.01±0.37 NeuN positive neurons per 62500 μm3. In contrast, mice expressing dnSNARE had an average of 4.33±1.17 NeuN positive neurons per 62500 μm3 of infarct region, demonstrating that dnSNARE mice had significantly greater numbers of neurons in the infarct region (p=0.039; Figure 2C).

Figure 2
Mice expressing dnSNARE show sparing of neurons in infarct and peri-infarct regions following photothrombosis

Assessments of NeuN positive cells in the peri-infarct region also showed significantly greater numbers in dnSNARE mice (11.83±1.74) compared to wildtype mice (8.17±1.01; p=0.024; Figure 2C). No difference was observed in the number of NeuN positive neurons per 62500 μm3 of contralateral hemisphere sensory-motor cortex between wildtype littermate (15.17±0.98) and dnSNARE (15.33±0.76) mice subjected to photothrombosis (Figure 2C).

Mice expressing dnSNARE show better performance on sensory-motor tasks following photothrombosis

To assess the functional impact of the photothrombotic lesions, we assessed animals pre-lesion to establish a baseline and again 24 hours post-lesion. We used two sensory-motor tasks that have been documented to demonstrate lesion severity, the rung walk ((Farr et al., 2006); Figure 3A) and the adhesive dot removal test ((Schallert and Whishaw, 1984); Figure 3C). Pre-lesion mice show very few errors during trials on the rung walk with wildtype mice showing an average of 0.14 ± 0.06 errors / steps, and dnSNARE mice showing 0.15 ± 0.10 errors / steps (Figure 3B). In contrast, 24 hours post lesion, wildtype mice show a significant increase in the number of errors / steps (12.41 ± 1.69) compared to pre lesion (p<0.001; Figure 3B). dnSNARE mice also show a significant increase in the number of errors / steps 24 hours post lesion (7.57 ± 1.06; p<0.001) compared to pre lesion. However compared to wildtype mice (12.41 ± 1.69), dnSNARE mice show significantly fewer errors post-lesion (7.57 ± 1.06; p =0.002; Figure 3B).

Figure 3
Mice expressing dnSNARE perform significantly better following photothrombosis on sensory-motor tasks compared to matched wildtype controls

In the adhesive dot removal test, pre-lesion animals were able to remove the adhesive dot in less than 2 seconds (1.98 ± 0.30 seconds wildtype; 2.03 ± 0.35 seconds dnSNARE; Figure 3D). At 24 hours post-lesion, wildtype mice take an average of 12.32 ± 1.90 seconds to remove the adhesive dot, while dnSNARE mice take an average of 7.34 ±1.01 seconds, both of which are significantly increased compared to pre-lesion times (p<0.001; Figure 3B). Comparison of wildtype (12.32 ± 1.90s) and dnSNARE (7.34 ± 1.01s) post-lesion reveals a significant difference (p=0.004) in post-lesion performance (Figure 3B).

Involvement of an NMDA signaling pathway

It is well known that cerebral ischemia can trigger NMDA receptor-mediated excitotoxicity, and indeed NMDA lesions are used as a model of the excitotoxic component of ischemic damage (Meldrum and Garthwaite, 1990; Kim et al., 2006; Lipton, 2007). Because astrocytic dnSNARE expression leads to reduced neuronal NMDA receptor expression, we asked whether dnSNARE expression would be protective against NMDA induced lesions. Mice were injected unilaterally with 1 μL of a 10 mg/mL solution of NMDA into the sensorimotor cortex and 24 hours later were sacrificed and NMDA lesion volume was assessed (Figure 4A). Mice expressing dnSNARE within astrocytes had a significantly reduced NMDA lesion volume (0.131± 0.0129 mm3) compared to wildtype mice (0.207±0.0260 mm3; p=0.040) as assessed by Nissl staining (Figure 4A,C).

Figure 4
Damage following NMDA lesions is attenuated by dnSNARE expression in astrocytes

Tissue sections from mice that had undergone unilateral NMDA lesions were also processed for immunohistochemistry for NeuN (Figure 4B). Within the NMDA lesion region, wildtype mice had an average of 0.96±0.26 NeuN positive neurons per 62500 μm3 (±0.26; Figure 4 D). In contrast, mice expressing dnSNARE had an average of 5.50±1.48 NeuN positive neurons per 62500 μm3 demonstrating that dnSNARE mice had significantly greater numbers of spared neurons within the NMDA lesion (p=0.024; Figure 4D). No difference was observed in the number of NeuN positive neurons per 62500 μm3 in cortical tissue contralateral to the NMDA lesion between wildtype littermate (14.83±1.70) and dnSNARE (15.67±1.33) mice.

Discussion

Astrocytes are well poised to impact the outcome of stroke because they are both structurally and functionally linked to both neurons and the vasculature (Haydon and Carmignoto, 2006; Attwell et al., 2010). From the data presented in this study we propose that an astrocytic SNARE signaling pathway plays a key role in the extent of damage following ischemic insult. We report that dnSNARE animals have decreased infarct volume, and increased sparing of NeuN positive neurons following photothrombotic damage in both the infarct and peri-infarct regions. We also observed a functional sparing in dnSNARE mice when animals that had undergone photothrombotic injury were tested 24 hours after lesion induction. Using the rung walk task and the adhesive dot removal tests we demonstrate that the sensorimotor areas have been functionally spared in the dnSNARE mice compared to wildtype mice receiving matched lesions.

There are many mechanisms that could mediate this protective effect. Previous studies using dnSNARE mice have shown that astrocytes regulate neuronal NMDA receptor trafficking. By releasing adenosine, astrocytes activate neuronal A1R that in turn stimulates a src family kinase dependent regulation of NMDA receptor trafficking. When dnSNARE is expressed in astrocytes this glial source of adenosine is lost leading to a reduction in neuronal NMDA receptors (Deng et al., 2011). Consequently, we propose that the ability of astrocytic dnSNARE expression to be neuroprotective is mediated in part by the loss of neuronal NMDA receptors. In agreement with this possibility we found that the magnitude of NMDA induced lesions is reduced in dnSNARE mice.

In addition to affects mediated by reduced cell surface NMDA receptors is the possibility that a dnSNARE dependent reduction in the Ca2+ dependent release of D-serine from astrocytes leads to a reduction in NMDA receptor mediated currents and thus to neuronal protection. For example, we have shown dnSNARE mice exhibit reduced D-serine regulation of NMDA receptors: in dnSNARE mice exogenous D-serine led to a greater augmentation of the NMDA receptor mediated current than in wildtype littermates (Fellin et al., 2009). In support of this possibility we have shown a significant reduction of infarct volume of 47% by decreasing astrocytic Ca2+ suggesting that the increased astrocytic Ca2+ signals in astrocytes contribute to ischemic damage (Ding et al., 2009).

The changes observed by (Ding et al., 2009) are particularly interesting because enhanced astrocytic calcium have been shown to induce the release of glutamate and d-serine which interact with the NR2B subunit of NMDA receptors (Araque et al., 1998; Parpura and Haydon, 2000; Aguado et al., 2002; Angulo et al., 2004; Fellin et al., 2004). We have previously shown that following status epilepticus, enhanced astrocytic Ca2+ signals could activate neuronal NR2B subunit of N-methyl d-aspartate (NMDA) receptors, resulting in delayed neuronal death (Ding et al., 2009).

Based on animal models of focal ischemia, numerous mechanisms of damage have been proposed including glutamate mediated exocytosis, oxidative stress, changes in pH and acidosis, and changes in mitochondrial function (Barber et al., 2003; Mattiasson et al., 2003; Xiong et al., 2004; Eltzschig and Eckle, 2011). In particular, the NMDA receptor has been shown to be critical for excitotoxicity following ischemia, and has been shown to increase intracellular Ca2+ levels and the initiation of both necrotic and apoptotic pathways (Szatkowski and Attwell, 1994; Martin et al., 1998; Aarts et al., 2003; Arundine and Tymianski, 2003, 2004). That a manipulation in the astrocytic dnSNARE signaling pathway can disrupt this process suggests that astrocytes impact cell loss that arises from ischemic injuries.

Acknowledgments

This work was supported by grants from the NIH to PGH (R01 NS037585, R01 MH095385) and by The Heart and Stroke Foundation of Canada - to DJH.

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