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Mol Cell Biol. Sep 1987; 7(9): 3365–3370.
PMCID: PMC367981

One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes.


A system of epithelial cells is described in which it is possible to study the number and the nature of genes capable of conferring the malignant phenotype. Two fully differentiated, hormone-responsive cell lines from rat thyroid glands are presented which are susceptible to one-step or two-step transformation upon infection with several murine acute retroviruses. After infection, both cell lines became independent from their thyrotropic hormone requirement for growth. However, complete transformation was achieved with one of the cell lines (FRTL-5 Cl 2), whereas the other cell line (PC Cl 3) failed to grow in agar and to give rise to tumors in vivo. The latter cell line was susceptible to complete transformation upon cooperation of the v-ras-Ha and the human c-myc oncogenes.

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Selected References

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  • Aaronson SA, Barbacid M. Origin and biological properties of a new BALB/c mouse sarcoma virus. J Virol. 1978 Aug;27(2):366–373. [PMC free article] [PubMed]
  • Adams SL, Sobel ME, Howard BH, Olden K, Yamada KM, de Crombrugghe B, Pastan I. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3399–3403. [PMC free article] [PubMed]
  • Ambesi-Impiombato FS, Parks LA, Coon HG. Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3455–3459. [PMC free article] [PubMed]
  • Anderson SM, Scolnick EM. Construction and isolation of a transforming murine retrovirus containing the src gene of Rous sarcoma virus. J Virol. 1983 May;46(2):594–605. [PMC free article] [PubMed]
  • Bechade C, Calothy G, Pessac B, Martin P, Coll J, Denhez F, Saule S, Ghysdael J, Stéhelin D. Induction of proliferation or transformation of neuroretina cells by the mil and myc viral oncogenes. Nature. 1985 Aug 8;316(6028):559–562. [PubMed]
  • Cairns J, Logan J. Step by step into carcinogenesis. Nature. 1983 Aug 18;304(5927):582–583. [PubMed]
  • Chirigos MA, Scott D, Turner W, Perk K. Biological, pathological and physical characterization of a possible variant of a murine sarcoma virus (Moloney). Int J Cancer. 1968 Mar 15;3(2):223–227. [PubMed]
  • Colletta G, Pinto A, Di Fiore PP, Fusco A, Ferrentino M, Avvedimento VE, Tsuchida N, Vecchio G. Dissociation between transformed and differentiated phenotype in rat thyroid epithelial cells after transformation with a temperature-sensitive mutant of the Kirsten murine sarcoma virus. Mol Cell Biol. 1983 Nov;3(11):2099–2109. [PMC free article] [PubMed]
  • DeLorbe WJ, Luciw PA, Goodman HM, Varmus HE, Bishop JM. Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. J Virol. 1980 Oct;36(1):50–61. [PMC free article] [PubMed]
  • Ellis RW, Defeo D, Shih TY, Gonda MA, Young HA, Tsuchida N, Lowy DR, Scolnick EM. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes. Nature. 1981 Aug 6;292(5823):506–511. [PubMed]
  • Franz T, Löhler J, Fusco A, Pragnell I, Nobis P, Padua R, Ostertag W. Transformation of mononuclear phagocytes in vivo and malignant histiocytosis caused by a novel murine spleen focus-forming virus. Nature. 1985 May 9;315(6015):149–151. [PubMed]
  • Fusco A, Pinto A, Ambesi-Impiombato FS, Vecchio G, Tsuchida N. Transformation of rat thyroid epithelial cells by Kirsten murine sarcoma virus. Int J Cancer. 1981 Nov 15;28(5):655–662. [PubMed]
  • Fusco A, Pinto A, Tramontano D, Tajana G, Vecchio G, Tsuchida N. Block in the expression of differentiation markers of rat thyroid epithelial cells by transformation with Kirsten murine sarcoma virus. Cancer Res. 1982 Feb;42(2):618–626. [PubMed]
  • Fusco A, Portella G, Di Fiore PP, Berlingieri MT, Di Lauro R, Schneider AB, Vecchio G. A mos oncogene-containing retrovirus, myeloproliferative sarcoma virus, transforms rat thyroid epithelial cells and irreversibly blocks their differentiation pattern. J Virol. 1985 Oct;56(1):284–292. [PMC free article] [PubMed]
  • Kaplan PL, Ozanne B. Cellular responsiveness to growth factors correlates with a cell's ability to express the transformed phenotype. Cell. 1983 Jul;33(3):931–938. [PubMed]
  • Land H, Parada LF, Weinberg RA. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature. 1983 Aug 18;304(5927):596–602. [PubMed]
  • Nishikura K, ar-Rushdi A, Erikson J, Watt R, Rovera G, Croce CM. Differential expression of the normal and of the translocated human c-myc oncogenes in B cells. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4822–4826. [PMC free article] [PubMed]
  • Peters RL, Rabstein LS, VanVleck R, Kelloff GJ, Huebner RJ. Naturally occurring sarcoma virus of the BALB/cCr mouse. J Natl Cancer Inst. 1974 Dec;53(6):1725–1729. [PubMed]
  • Rapp UR, Goldsborough MD, Mark GE, Bonner TI, Groffen J, Reynolds FH, Jr, Stephenson JR. Structure and biological activity of v-raf, a unique oncogene transduced by a retrovirus. Proc Natl Acad Sci U S A. 1983 Jul;80(14):4218–4222. [PMC free article] [PubMed]
  • Rapp UR, Reynolds FH, Jr, Stephenson JR. New mammalian transforming retrovirus: demonstration of a polyprotein gene product. J Virol. 1983 Mar;45(3):914–924. [PMC free article] [PubMed]
  • Rasheed S, Gardner MB, Huebner RJ. In vitro isolation of stable rat sarcoma viruses. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2972–2976. [PMC free article] [PubMed]
  • Spandidos DA. Mechanism of carcinogenesis: the role of oncogenes, transcriptional enhancers and growth factors. Anticancer Res. 1985 Sep-Oct;5(5):485–498. [PubMed]
  • Spandidos DA, Wilkie NM. Malignant transformation of early passage rodent cells by a single mutated human oncogene. Nature. 1984 Aug 9;310(5977):469–475. [PubMed]
  • Thomas PS. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. [PMC free article] [PubMed]
  • Watson DK, Psallidopoulos MC, Samuel KP, Dalla-Favera R, Papas TS. Nucleotide sequence analysis of human c-myc locus, chicken homologue, and myelocytomatosis virus MC29 transforming gene reveals a highly conserved gene product. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3642–3645. [PMC free article] [PubMed]

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