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Mol Cell Biol. 1987 September; 7(9): 3199–3204. | PMCID: PMC367955 |
A Podospora anserina longevity mutant with a temperature-sensitive phenotype for senescence. M S Turker, J G Nelson, and D J Cummings Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver 80262. Abstract A Podospora anserina longevity mutant was identified with a temperature-sensitive phenotype for senescence. This mutant, termed TS1, grew for over 3 m at 27 degrees C, but when shifted to 34 degrees C, it underwent senescence between 10 and 18 cm. A previously described senescence-associated plasmid, alpha senDNA, derived from the mitochondrial genome, was not detected in TS1 at 27 degrees C but was present in senescent cultures at 34 degrees C. A similar result was observed in progeny strains obtained by crossing the TS1 mutant with a wild-type strain. Other mitochondrial excision-amplification DNAs in addition to alpha senDNA were also observed in the senescent cultures. Most were derived from a specific region of the mitochondrial genome. These results provide evidence that alpha senDNA is involved in TS1 senescence and suggest that this plasmid may play a role in the formation of other mitochondrial excision-amplification plasmids. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - Belcour Léon, Vierny Corinne. Variable DNA splicing sites of a mitochondrial intron: relationship to the senescence process in Podospora. EMBO J. 1986 Mar;5(3):609–614. [PubMed]
- Cummings DJ, Belcour L, Grandchamp C. Mitochondrial DNA from Podospora anserina. I. Isolation and characterization. Mol Gen Genet. 1979 Mar 27;171(3):229–238. [PubMed]
- Cummings DJ, Belcour L, Grandchamp C. Mitochondrial DNA from Podospora anserina. II. Properties of mutant DNA and multimeric circular DNA from senescent cultures. Mol Gen Genet. 1979 Mar 27;171(3):239–250. [PubMed]
- Jamet-Vierny C, Begel O, Belcour L. Senescence in Podospora anserina: amplification of a mitochondrial DNA sequence. Cell. 1980 Aug;21(1):189–194. [PubMed]
- Koll F, Belcour L, Vierny C. A 1100-bp sequence of mitochondrial DNA is involved in senescence process in Podospora: study of senescent and mutant cultures. Plasmid. 1985 Sep;14(2):106–117. [PubMed]
- Kück U, Osiewacz HD, Schmidt U, Kappelhoff B, Schulte E, Stahl U, Esser K. The onset of senescence is affected by DNA rearrangements of a discontinuous mitochondrial gene in Podospora anserina. Curr Genet. 1985;9(5):373–382. [PubMed]
- Matsuura ET, Domenico JM, Cummings DJ. An additional class II intron with homology to reverse transcriptase in rapidly senescing Podospora anserina. Curr Genet. 1986;10(12):915–922. [PubMed]
- Michel F, Lang BF. Mitochondrial class II introns encode proteins related to the reverse transcriptases of retroviruses. Nature. 316(6029):641–643. [PubMed]
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- Stahl U, Lemke PA, Tudzynski P, Kück U, Esser K. Evidence for plasmid like DNA in a filamentous fungus, the ascomycete Podospora anserina. Mol Gen Genet. 1978 Jul 4;162(3):341–343. [PubMed]
- Stahl U, Tudzynski P, Kück U, Esser K. Replication and expression of a bacterial--mitochondrial hybrid plasmid in the fungus Podospora anserina. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3641–3645. [PubMed]
- Steinhilber W, Cummings DJ. A DNA polymerase activity with characteristics of a reverse transcriptase in Podospora anserina. Curr Genet. 1986;10(5):389–392. [PubMed]
- Turker MS, Cummings DJ. Podospora anserina does not senesce when serially passaged in liquid culture. J Bacteriol. 1987 Feb;169(2):454–460. [PubMed]
- Turker MS, Domenico JM, Cummings DJ. A novel family of mitochondrial plasmids associated with longevity mutants of Podospora anserina. J Biol Chem. 1987 Feb 15;262(5):2250–2255. [PubMed]
- Vierny C, Keller AM, Begel O, Belcour L. A sequence of mitochondrial DNA is associated with the onset of senescence in a fungus. Nature. 1982 May 13;297(5862):157–159. [PubMed]
- Wright RM, Horrum MA, Cummings DJ. Are mitochondrial structural genes selectively amplified during senescence in Podospora anserina? Cell. 1982 Jun;29(2):505–515. [PubMed]
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