Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
PMC full text:
Cell. Author manuscript; available in PMC 2013 Aug 3.
Published in final edited form as:
Cell. 2012 Aug 3; 150(3): 606–619.
Published online 2012 Jul 19. doi:  10.1016/j.cell.2012.07.007

Figure 2

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TRIF Signaling Downstream of TLR4-TRAM Mediates Inflammasome Activation by EHEC and C. rodentium

(A) Cleaved caspase-11 in the supernatants of Pam3CSK4-primed BMDMs stimulated for 16 hr with EHEC (MOI of 25 and 50), C. rodentium (MOI of 25 and 50), or polydAdT.

(B, D, and F–J) IL-1β or IL-1α production by Pam3CSK4-primed BMDMs from C57BL/6 wild-type and various knockout mice stimulated with EHEC, C. rodentium, polydAdT, or silica for 16 hr or nigericin for 1 hr.

(C) Cell death in BMDMs stimulated with EHEC, C. rodentium, polydAdT, or silica for 16 hr.

(E) Immunoblots for cleaved caspase-11, caspase-1, and IL-1β in the supernatants of Pam3CSK4-primed BMDMs stimulated with EHEC, C. rodentium, polydAdT, or silica for 16 hr.

Data are presented as the mean ± SEM of one experiment representative of three experiments. See also Figure S2.

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