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Journal List > Plant Physiol > v.49(5); May 1972

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Plant Physiol. 1972 May; 49(5): 852–856.
PMCID: PMC366064
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Effect of Filipin on Liposomes Prepared with Different Types of Steroids 1
M. G. Kleinschmidta and K. S. Chougha
J. B. Muddb
aDepartment of Agricultural Chemistry, Washington State University, Pullman, Washington 99163
Department of Biochemistry and Air Pollution Research Center, University of California, Riverside, California 92502
1 Scientific Paper No. 3744, Project 1634, College of Agriculture, Washington State University, Pullman, Wash. 99163.
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Abstract
The action of the polyene antibiotic filipin on the permeability of liposomes prepared with lecithin and several plant and other steroids was studied. The effect of filipin was found to be dependent upon the type of steroid incorporated into the membrane. The interaction of filipin with steroids was found to be related both to the functional group at the 3-position and the aliphatic chain of the steroid. Complex formation of the steroid with filipin in aqueous solutions, as detected by ultraviolet spectrophotometry, correlated with the ability of filipin to increase the permeability of the liposomes.
From these results it was suggested that the action of filipin could be used as a diagnostic tool to determine the presence of different steroids in plant membranes. Differences in the sensitivity of subcellular organelles to filipin may be due to differences in the steroid composition of their membranes.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
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