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J Clin Microbiol. Apr 1986; 23(4): 768–771.
PMCID: PMC362834

Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies.


A panel of monoclonal antibodies to Legionella pneumophila serogroup 1 and a subclassification scheme were developed in a collaborative project among three laboratories. The seven most useful monoclonal antibodies were selected from three previously developed panels on the basis of indirect fluorescent antibody patterns with 83 strains of L. pneumophila serogroup 1 that were obtained from widely distributed geographic locations. The isolates were divided into 10 major subgroups on the basis of reactivity patterns that can be readily reproduced in any laboratory and are not subject to major inconsistencies of interpretation of staining intensity. A standard protocol for the indirect fluorescent antibody procedure was also developed.

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  • Bibb WF, Arnow PM, Dellinger DL, Perryman SR. Isolation and characterization of a seventh serogroup of Legionella pneumophila. J Clin Microbiol. 1983 Feb;17(2):346–348. [PMC free article] [PubMed]
  • Bibb WF, Arnow PM, Thacker L, McKinney RM. Detection of soluble Legionella pneumophila antigens in serum and urine specimens by enzyme-linked immunosorbent assay with monoclonal and polyclonal antibodies. J Clin Microbiol. 1984 Sep;20(3):478–482. [PMC free article] [PubMed]
  • Bissett ML, Lee JO, Lindquist DS. New serogroup of Legionella pneumophila, serogroup 8. J Clin Microbiol. 1983 May;17(5):887–891. [PMC free article] [PubMed]
  • Brown A, Vickers RM, Elder EM, Lema M, Garrity GM. Plasmid and surface antigen markers of endemic and epidemic Legionella pneumophila strains. J Clin Microbiol. 1982 Aug;16(2):230–235. [PMC free article] [PubMed]
  • Cordes LG, Fraser DW, Skaliy P, Perlino CA, Elsea WR, Mallison GF, Hayes PS. Legionnaires' disease outbreak at an Atlanta, Georgia, Country Club: evidence for spread from an evaporative condenser. Am J Epidemiol. 1980 Apr;111(4):425–431. [PubMed]
  • Dennis PJ, Taylor JA, Fitzgeorge RB, Bartlett CL, Barrow GI. Legionella pneumophila in water plumbing systems. Lancet. 1982 Apr 24;1(8278):949–951. [PubMed]
  • Dondero TJ, Jr, Rendtorff RC, Mallison GF, Weeks RM, Levy JS, Wong EW, Schaffner W. An outbreak of Legionnaires' disease associated with a contaminated air-conditioning cooling tower. N Engl J Med. 1980 Feb 14;302(7):365–370. [PubMed]
  • Edelstein PH, Bibb WF, Gorman GW, Thacker WL, Brenner DJ, Wilkinson HW, Moss CW, Buddington RS, Dunn CJ, Roos PJ, et al. Legionella pneumophila serogroup 9: a cause of human pneumonia. Ann Intern Med. 1984 Aug;101(2):196–198. [PubMed]
  • England AC, 3rd, McKinney RM, Skaliy P, Gorman GW. A fifth serogroup of Legionella pneumophila. Ann Intern Med. 1980 Jul;93(1):58–59. [PubMed]
  • Fliermans CB, Cherry WB, Orrison LH, Smith SJ, Tison DL, Pope DH. Ecological distribution of Legionella pneumophila. Appl Environ Microbiol. 1981 Jan;41(1):9–16. [PMC free article] [PubMed]
  • Fraser DW, Tsai TR, Orenstein W, Parkin WE, Beecham HJ, Sharrar RG, Harris J, Mallison GF, Martin SM, McDade JE, et al. Legionnaires' disease: description of an epidemic of pneumonia. N Engl J Med. 1977 Dec 1;297(22):1189–1197. [PubMed]
  • Joly JR, Chen YY, Ramsay D. Serogrouping and subtyping of Legionella pneumophila with monoclonal antibodies. J Clin Microbiol. 1983 Nov;18(5):1040–1046. [PMC free article] [PubMed]
  • Joly JR, Winn WC. Correlation of subtypes of Legionella pneumophila defined by monoclonal antibodies with epidemiological classification of cases and environmental sources. J Infect Dis. 1984 Nov;150(5):667–671. [PubMed]
  • Lema M, Brown A. Electrophoretic characterization of soluble protein extracts of Legionella pneumophila and other members of the family Legionellaceae. J Clin Microbiol. 1983 Jun;17(6):1132–1140. [PMC free article] [PubMed]
  • McDade JE, Shepard CC, Fraser DW, Tsai TR, Redus MA, Dowdle WR. Legionnaires' disease: isolation of a bacterium and demonstration of its role in other respiratory disease. N Engl J Med. 1977 Dec 1;297(22):1197–1203. [PubMed]
  • McKinney RM, Spillane JT. An approach to quantitation in rhodamine isothiocyanate labeling. Ann N Y Acad Sci. 1975 Jun 30;254:55–64. [PubMed]
  • McKinney RM, Thacker L, Harris PP, Lewallen KR, Hebert GA, Edelstein PH, Thomason BM. Four serogroups of Legionnaires' disease bacteria defined by direct immunofluorescence. Ann Intern Med. 1979 Apr;90(4):621–624. [PubMed]
  • McKinney RM, Thacker L, Wells DE, Wong MC, Jones WJ, Bibb WF. Monoclonal antibodies to Legionella pneumophila serogroup 1: possible applications in diagnostic tests and epidemiologic studies. Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Jul;255(1):91–95. [PubMed]
  • McKinney RM, Thomason BM, Harris PP, Thacker L, Lewallen KR, Wilkinson HW, Hebert GA, Moss CW. Recognition of a new serogroup of Legionnaires disease bacterium. J Clin Microbiol. 1979 Jan;9(1):103–107. [PMC free article] [PubMed]
  • McKinney RM, Wilkinson HW, Sommers HM, Fikes BJ, Sasseville KR, Yungbluth MM, Wolf JS. Legionella pneumophila serogroup six: isolation from cases of legionellosis, identification by immunofluorescence staining, and immunological response to infection. J Clin Microbiol. 1980 Sep;12(3):395–401. [PMC free article] [PubMed]
  • Meenhorst PL, Reingold AL, Groothuis DG, Gorman GW, Wilkinson HW, McKinney RM, Feeley JC, Brenner DJ, van Furth R. Water-related nosocomial pneumonia caused by Legionella pneumophila serogroups 1 and 10. J Infect Dis. 1985 Aug;152(2):356–364. [PubMed]
  • Para MF, Plouffe JF. Production of monoclonal antibodies to Legionella pneumophila serogroups 1 and 6. J Clin Microbiol. 1983 Oct;18(4):895–900. [PMC free article] [PubMed]
  • Plouffe JF, Para MF, Maher WE, Hackman B, Webster L. Subtypes of Legionella pneumophila serogroup 1 associated with different attack rates. Lancet. 1983 Sep 17;2(8351):649–650. [PubMed]
  • Reingold AL, Thomason BM, Brake BJ, Thacker L, Wilkinson HW, Kuritsky JN. Legionella pneumonia in the United States: the distribution of serogroups and species causing human illness. J Infect Dis. 1984 May;149(5):819–819. [PubMed]
  • Stout J, Yu VL, Vickers RM, Zuravleff J, Best M, Brown A, Yee RB, Wadowsky R. Ubiquitousness of Legionella pneumophila in the water supply of a hospital with endemic Legionnaires' disease. N Engl J Med. 1982 Feb 25;306(8):466–468. [PubMed]
  • Thomason BM, Bibb WF. Use of absorbed antisera for demonstration of antigenic variation among strains of Legionella pneumophila serogroup 1. J Clin Microbiol. 1984 Jun;19(6):794–797. [PMC free article] [PubMed]
  • Tobin JO, Beare J, Dunnill MS, Fisher-Hoch S, French M, Mitchell RG, Morris PJ, Muers MF. Legionnaires' disease in a transplant unit: isolation of the causative agent from shower baths. Lancet. 1980 Jul 19;2(8186):118–121. [PubMed]
  • Watkins ID, Tobin JO, Dennis PJ, Brown W, Newnham R, Kurtz JB. Legionella pneumophila serogroup 1 subgrouping by monoclonal antibodies--an epidemiological tool. J Hyg (Lond) 1985 Oct;95(2):211–216. [PMC free article] [PubMed]
  • Zanen-Lim OG, van den Broek NJ, Rietra PJ, Zanen HC. Comparison of strains of Legionella pneumophila serogroup 1 isolated in four Amsterdam hospitals from patients and hot-water supplies. J Infect Dis. 1984 Oct;150(4):508–512. [PubMed]

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