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Mol Cell Biol. Jul 1989; 9(7): 3151–3154.
PMCID: PMC362792

Integral membrane protein located in the apical complex of Plasmodium falciparum.


We describe the cloning of a novel antigen of Plasmodium falciparum which contains a hydrophobic domain typical of an integral membrane protein. This antigen is designated apical membrane antigen 1 because it appears to be located in the apical complex. Apical membrane antigen 1 appears to be transported to the merozoite surface near the time of schizont rupture.

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  • Aikawa M, Miller LH, Johnson J, Rabbege J. Erythrocyte entry by malarial parasites. A moving junction between erythrocyte and parasite. J Cell Biol. 1978 Apr;77(1):72–82. [PMC free article] [PubMed]
  • Ardeshir F, Flint JE, Richman SJ, Reese RT. A 75 kd merozoite surface protein of Plasmodium falciparum which is related to the 70 kd heat-shock proteins. EMBO J. 1987 Feb;6(2):493–499. [PMC free article] [PubMed]
  • Bannister LH, Butcher GA, Dennis ED, Mitchell GH. Structure and invasive behaviour of Plasmodium knowlesi merozoites in vitro. Parasitology. 1975 Dec;71(3):483–491. [PubMed]
  • Bannister LH, Mitchell GH, Butcher GA, Dennis ED. Lamellar membranes associated with rhoptries in erythrocytic merozoites of Plasmodium knowlesi: a clue to the mechanism of invasion. Parasitology. 1986 Apr;92(Pt 2):291–303. [PubMed]
  • Braun-Breton C, Rosenberry TL, da Silva LP. Induction of the proteolytic activity of a membrane protein in Plasmodium falciparum by phosphatidyl inositol-specific phospholipase C. Nature. 1988 Mar 31;332(6163):457–459. [PubMed]
  • Brown GV, Culvenor JG, Crewther PE, Bianco AE, Coppel RL, Saint RB, Stahl HD, Kemp DJ, Anders RF. Localization of the ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum in merozoites and ring-infected erythrocytes. J Exp Med. 1985 Aug 1;162(2):774–779. [PMC free article] [PubMed]
  • Bzik DJ, Li WB, Horii T, Inselburg J. Amino acid sequence of the serine-repeat antigen (SERA) of Plasmodium falciparum determined from cloned cDNA. Mol Biochem Parasitol. 1988 Sep;30(3):279–288. [PubMed]
  • Campbell GH, Miller LH, Hudson D, Franco EL, Andrysiak PM. Monoclonal antibody characterization of Plasmodium falciparum antigens. Am J Trop Med Hyg. 1984 Nov;33(6):1051–1054. [PubMed]
  • Crewther PE, Bianco AE, Brown GV, Coppel RL, Stahl HD, Kemp DJ, Anders RF. Affinity purification of human antibodies directed against cloned antigens of Plasmodium falciparum. J Immunol Methods. 1986 Feb 12;86(2):257–264. [PubMed]
  • Freeman RR, Trejdosiewicz AJ, Cross GA. Protective monoclonal antibodies recognising stage-specific merozoite antigens of a rodent malaria parasite. Nature. 1980 Mar 27;284(5754):366–368. [PubMed]
  • Holder AA, Freeman RR. Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody. J Exp Med. 1982 Nov 1;156(5):1528–1538. [PMC free article] [PubMed]
  • Holder AA, Freeman RR, Uni S, Aikawa M. Isolation of a Plasmodium falciparum rhoptry protein. Mol Biochem Parasitol. 1985 Mar;14(3):293–303. [PubMed]
  • Ladda R, Aikawa M, Sprinz H. Penetration of erythrocytes by merozoites of mammalian and avian malarial parasites. J Parasitol. 1969 Jun;55(3):633–644. [PubMed]
  • Lustigman S, Anders RF, Brown GV, Coppel RL. A component of an antigenic rhoptry complex of Plasmodium falciparum is modified after merozoite invasion. Mol Biochem Parasitol. 1988 Sep;30(3):217–224. [PubMed]
  • Oka M, Aikawa M, Freeman RR, Holder AA, Fine E. Ultrastructural localization of protective antigens of Plasmodium yoelii merozoites by the use of monoclonal antibodies and ultrathin cryomicrotomy. Am J Trop Med Hyg. 1984 May;33(3):342–346. [PubMed]
  • Perkins ME. Surface proteins of Plasmodium falciparum merozoites binding to the erythrocyte receptor, glycophorin. J Exp Med. 1984 Sep 1;160(3):788–798. [PMC free article] [PubMed]
  • Perrin LH, Merkli B, Gabra MS, Stocker JW, Chizzolini C, Richle R. Immunization with a Plasmodium falciparum merozoite surface antigen induces a partial immunity in monkeys. J Clin Invest. 1985 May;75(5):1718–1721. [PMC free article] [PubMed]
  • Roger N, Dubremetz JF, Delplace P, Fortier B, Tronchin G, Vernes A. Characterization of a 225 kilodalton rhoptry protein of Plasmodium falciparum. Mol Biochem Parasitol. 1988 Jan 15;27(2-3):135–141. [PubMed]
  • Schofield L, Bushell GR, Cooper JA, Saul AJ, Upcroft JA, Kidson C. A rhoptry antigen of Plasmodium falciparum contains conserved and variable epitopes recognized by inhibitory monoclonal antibodies. Mol Biochem Parasitol. 1986 Feb;18(2):183–195. [PubMed]
  • Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988 Jul 15;67(1):31–40. [PubMed]
  • Smythe JA, Coppel RL, Brown GV, Ramasamy R, Kemp DJ, Anders RF. Identification of two integral membrane proteins of Plasmodium falciparum. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5195–5199. [PMC free article] [PubMed]
  • Stahl HD, Bianco AE, Crewther PE, Burkot T, Coppel RL, Brown GV, Anders RF, Kemp DJ. An asparagine-rich protein from blood stages of Plasmodium falciparum shares determinants with sporozoites. Nucleic Acids Res. 1986 Apr 11;14(7):3089–3102. [PMC free article] [PubMed]
  • Stewart MJ, Schulman S, Vanderberg JP. Rhoptry secretion of membranous whorls by Plasmodium falciparum merozoites. Am J Trop Med Hyg. 1986 Jan;35(1):37–44. [PubMed]
  • Uni S, Masuda A, Stewart MJ, Igarashi I, Nussenzweig R, Aikawa M. Ultrastructural localization of the 150/130 Kd antigens in sexual and asexual blood stages of Plasmodium falciparum-infected human erythrocytes. Am J Trop Med Hyg. 1987 May;36(3):481–488. [PubMed]
  • Weber JL, Lyon JA, Wolff RH, Hall T, Lowell GH, Chulay JD. Primary structure of a Plasmodium falciparum malaria antigen located at the merozoite surface and within the parasitophorous vacuole. J Biol Chem. 1988 Aug 15;263(23):11421–11425. [PubMed]

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