• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of molcellbPermissionsJournals.ASM.orgJournalMCB ArticleJournal InfoAuthorsReviewers
Mol Cell Biol. Sep 1989; 9(9): 4018–4021.
PMCID: PMC362464

Trypanosoma brucei: posttranscriptional control of the variable surface glycoprotein gene expression site.

Abstract

The arrest of variable surface glycoprotein (VSG) synthesis is one of the first events accompanying the differentiation of Trypanosoma brucei bloodstream forms into procyclic forms, which are characteristic of the insect vector. This is because of a very fast inhibition of VSG gene transcription which occurs as soon as the temperature is lowered. We report that this effect is probably not controlled at the level of transcription initiation, since the beginning of the VSG gene expression site, about 45 kilobases upstream from the antigen gene, remains transcribed in procyclic forms. The permanent activity of the promoter readily accounts for the systematic reappearance, upon return to the bloodstream form after cyclical transmission, of the antigen type present before passage to the tsetse fly. The abortive transcription of the VSG gene expression site appears linked to RNA processing abnormalities. Such posttranscriptional controls may allow the modulation of gene expression in a genome organized in large multigenic transcription units.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Alexandre S, Guyaux M, Murphy NB, Coquelet H, Pays A, Steinert M, Pays E. Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei. Mol Cell Biol. 1988 Jun;8(6):2367–2378. [PMC free article] [PubMed]
  • Ben Amar MF, Pays A, Tebabi P, Dero B, Seebeck T, Steinert M, Pays E. Structure and transcription of the actin gene of Trypanosoma brucei. Mol Cell Biol. 1988 May;8(5):2166–2176. [PMC free article] [PubMed]
  • Bienen EJ, Hammadi E, Hill GC. Trypanosoma brucei: biochemical and morphological changes during in vitro transformation of bloodstream- to procyclic-trypomastigotes. Exp Parasitol. 1981 Jun;51(3):408–417. [PubMed]
  • Coquelet H, Tebabi P, Pays A, Steinert M, Pays E. Trypanosoma brucei: enrichment by UV of intergenic transcripts from the variable surface glycoprotein gene expression site. Mol Cell Biol. 1989 Sep;9(9):4022–4025. [PMC free article] [PubMed]
  • Czichos J, Ehlers B, Overath P. Regulation of variant surface glycoprotein synthesis in Trypanosoma brucei. Biochem Soc Trans. 1988 Jun;16(3):261–263. [PubMed]
  • Delauw MF, Pays E, Steinert M, Aerts D, Van Meirvenne N, Le Ray D. Inactivation and reactivation of a variant-specific antigen gene in cyclically transmitted Trypanosoma brucei. EMBO J. 1985 Apr;4(4):989–993. [PMC free article] [PubMed]
  • Ehlers B, Czichos J, Overath P. Repression and reactivation of the variant surface glycoprotein gene in Trypanosoma brucei. FEBS Lett. 1987 Dec 10;225(1-2):53–58. [PubMed]
  • Ehlers B, Czichos J, Overath P. RNA turnover in Trypanosoma brucei. Mol Cell Biol. 1987 Mar;7(3):1242–1249. [PMC free article] [PubMed]
  • Ghiotto V, Brun R, Jenni L, Hecker H. Trypanosoma brucei: morphometric changes and loss of infectivity during transformation of bloodstream forms to procyclic culture forms in vitro. Exp Parasitol. 1979 Dec;48(3):447–456. [PubMed]
  • Gibson WC, Swinkels BW, Borst P. Post-transcriptional control of the differential expression of phosphoglycerate kinase genes in Trypanosoma brucei. J Mol Biol. 1988 May 20;201(2):315–325. [PubMed]
  • Gubler U, Hoffman BJ. A simple and very efficient method for generating cDNA libraries. Gene. 1983 Nov;25(2-3):263–269. [PubMed]
  • Hasan G, Turner MJ, Cordingley JS. Complete nucleotide sequence of an unusual mobile element from trypanosoma brucei. Cell. 1984 May;37(1):333–341. [PubMed]
  • Johnson PJ, Kooter JM, Borst P. Inactivation of transcription by UV irradiation of T. brucei provides evidence for a multicistronic transcription unit including a VSG gene. Cell. 1987 Oct 23;51(2):273–281. [PubMed]
  • Kooter JM, van der Spek HJ, Wagter R, d'Oliveira CE, van der Hoeven F, Johnson PJ, Borst P. The anatomy and transcription of a telomeric expression site for variant-specific surface antigens in T. brucei. Cell. 1987 Oct 23;51(2):261–272. [PubMed]
  • Laurent M, Pays E, Magnus E, Van Meirvenne N, Matthyssens G, Williams RO, Steinert M. DNA rearrangements linked to expression of a predominant surface antigen gene of trypanosomes. Nature. 1983 Mar 17;302(5905):263–266. [PubMed]
  • Mitchelson K, Moss T. The enhancement of ribosomal transcription by the recycling of RNA polymerase I. Nucleic Acids Res. 1987 Nov 25;15(22):9577–9596. [PMC free article] [PubMed]
  • Murphy NB, Pays A, Tebabi P, Coquelet H, Guyaux M, Steinert M, Pays E. Trypanosoma brucei repeated element with unusual structural and transcriptional properties. J Mol Biol. 1987 Jun 20;195(4):855–871. [PubMed]
  • Overath P, Czichos J, Stock U, Nonnengaesser C. Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma brucei. EMBO J. 1983;2(10):1721–1728. [PMC free article] [PubMed]
  • Pays E, Delronche M, Lheureux M, Vervoort T, Bloch J, Gannon F, Steinert M. Cloning and characterization of DNA sequences complementary to messenger ribonucleic acids coding for the synthesis of two surface antigens of Trypanosoma brucei. Nucleic Acids Res. 1980 Dec 20;8(24):5965–5981. [PMC free article] [PubMed]
  • Pays E, Guyaux M, Aerts D, Van Meirvenne N, Steinert M. Telomeric reciprocal recombination as a possible mechanism for antigenic variation in trypanosomes. Nature. 1985 Aug 8;316(6028):562–564. [PubMed]
  • Pays E, Tebabi P, Pays A, Coquelet H, Revelard P, Salmon D, Steinert M. The genes and transcripts of an antigen gene expression site from T. brucei. Cell. 1989 Jun 2;57(5):835–845. [PubMed]
  • Pays E, Van Assel S, Laurent M, Dero B, Michiels F, Kronenberger P, Matthyssens G, Van Meirvenne N, Le Ray D, Steinert M. At least two transposed sequences are associated in the expression site of a surface antigen gene in different trypanosome clones. Cell. 1983 Sep;34(2):359–369. [PubMed]
  • Pearson TW, Moloo SK, Jenni L. Culture form and tsetse fly midgut form procyclic Trypanosoma brucei express common proteins. Mol Biochem Parasitol. 1987 Oct;25(3):273–278. [PubMed]
  • Roditi I, Carrington M, Turner M. Expression of a polypeptide containing a dipeptide repeat is confined to the insect stage of Trypanosoma brucei. Nature. 1987 Jan 15;325(6101):272–274. [PubMed]
  • Roditi I, Schwarz H, Pearson TW, Beecroft RP, Liu MK, Richardson JP, Bühring HJ, Pleiss J, Bülow R, Williams RO, et al. Procyclin gene expression and loss of the variant surface glycoprotein during differentiation of Trypanosoma brucei. J Cell Biol. 1989 Feb;108(2):737–746. [PMC free article] [PubMed]
  • Sanger F, Coulson AR, Barrell BG, Smith AJ, Roe BA. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. [PubMed]
  • Shea C, Lee MG, Van der Ploeg LH. VSG gene 118 is transcribed from a cotransposed pol I-like promoter. Cell. 1987 Aug 14;50(4):603–612. [PubMed]
  • Torri AF, Hajduk SL. Posttranscriptional regulation of cytochrome c expression during the developmental cycle of Trypanosoma brucei. Mol Cell Biol. 1988 Nov;8(11):4625–4633. [PMC free article] [PubMed]
  • Vickerman K. Developmental cycles and biology of pathogenic trypanosomes. Br Med Bull. 1985 Apr;41(2):105–114. [PubMed]

Articles from Molecular and Cellular Biology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...