Logo of molcellbPermissionsJournals.ASM.orgJournalMCB ArticleJournal InfoAuthorsReviewers
Mol Cell Biol. 1990 Jun; 10(6): 3243–3246.
PMCID: PMC360690

A and T homopolymeric stretches mediate a DNA inversion in Plasmodium falciparum which results in loss of gene expression.


Ring-infected erythrocyte surface antigen-negative isolates of Plasmodium falciparum demonstrate a complex DNA rearrangement with inversion of 5' coding sequences, deletion of upstream and flanking sequences, and healing of the truncated chromosome by telomere addition. An inversion intermediate that results in the telomeric gene structure for RESA has been identified in the pathway. This inversion creates a mitotically stable substrate for the sequence-specific addition of telomere repeats at the deletion breakpoint.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (950K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anders RF, Brown GV, Edwards A. Characterization of an S antigen synthesized by several isolates of Plasmodium falciparum. Proc Natl Acad Sci U S A. 1983 Nov;80(21):6652–6656. [PMC free article] [PubMed]
  • Brown GV, Culvenor JG, Crewther PE, Bianco AE, Coppel RL, Saint RB, Stahl HD, Kemp DJ, Anders RF. Localization of the ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum in merozoites and ring-infected erythrocytes. J Exp Med. 1985 Aug 1;162(2):774–779. [PMC free article] [PubMed]
  • Cappai R, van Schravendijk MR, Anders RF, Peterson MG, Thomas LM, Cowman AF, Kemp DJ. Expression of the RESA gene in Plasmodium falciparum isolate FCR3 is prevented by a subtelomeric deletion. Mol Cell Biol. 1989 Aug;9(8):3584–3587. [PMC free article] [PubMed]
  • Corcoran LM, Forsyth KP, Bianco AE, Brown GV, Kemp DJ. Chromosome size polymorphisms in Plasmodium falciparum can involve deletions and are frequent in natural parasite populations. Cell. 1986 Jan 17;44(1):87–95. [PubMed]
  • Favaloro JM, Coppel RL, Corcoran LM, Foote SJ, Brown GV, Anders RF, Kemp DJ. Structure of the RESA gene of Plasmodium falciparum. Nucleic Acids Res. 1986 Nov 11;14(21):8265–8277. [PMC free article] [PubMed]
  • Giphart-Gassler M, Plasterk RH, van de Putte P. G inversion in bacteriophage Mu: a novel way of gene splicing. Nature. 1982 May 27;297(5864):339–342. [PubMed]
  • Jensen JB, Trager W. Plasmodium falciparum in culture: establishment of additional strains. Am J Trop Med Hyg. 1978 Jul;27(4):743–746. [PubMed]
  • Kemp DJ, Thompson JK, Walliker D, Corcoran LM. Molecular karyotype of Plasmodium falciparum: conserved linkage groups and expendable histidine-rich protein genes. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7672–7676. [PMC free article] [PubMed]
  • Kemp DJ, Coppel RL, Cowman AF, Saint RB, Brown GV, Anders RF. Expression of Plasmodium falciparum blood-stage antigens in Escherichia coli: detection with antibodies from immune humans. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3787–3791. [PMC free article] [PubMed]
  • Pologe LG, Ravetch JV. A chromosomal rearrangement in a P. falciparum histidine-rich protein gene is associated with the knobless phenotype. Nature. 322(6078):474–477. [PubMed]
  • Pologe LG, Ravetch JV. Large deletions result from breakage and healing of P. falciparum chromosomes. Cell. 1988 Dec 2;55(5):869–874. [PubMed]
  • Trager W, Tershakovec M, Lyandvert L, Stanley H, Lanners N, Gubert E. Clones of the malaria parasite Plasmodium falciparum obtained by microscopic selection: their characterization with regard to knobs, chloroquine sensitivity, and formation of gametocytes. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6527–6530. [PMC free article] [PubMed]
  • Uni S, Masuda A, Stewart MJ, Igarashi I, Nussenzweig R, Aikawa M. Ultrastructural localization of the 150/130 Kd antigens in sexual and asexual blood stages of Plasmodium falciparum-infected human erythrocytes. Am J Trop Med Hyg. 1987 May;36(3):481–488. [PubMed]
  • Van der Ploeg LH, Smits M, Ponnudurai T, Vermeulen A, Meuwissen JH, Langsley G. Chromosome-sized DNA molecules of Plasmodium falciparum. Science. 1985 Aug 16;229(4714):658–661. [PubMed]
  • Zeff RA, Gopas J, Steinhauer E, Rajan TV, Nathenson SG. Analysis of somatic cell H-2 variants to define the structural requirements for class I antigen expression. J Immunol. 1986 Aug 1;137(3):897–903. [PubMed]
  • Zieg J, Simon M. Analysis of the nucleotide sequence of an invertible controlling element. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4196–4200. [PMC free article] [PubMed]

Articles from Molecular and Cellular Biology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...