Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Jul 1976; 19(1): 208–219.
PMCID: PMC354848

Prophage map of converting corynebacteriophage beta.


A prophage map for corynebacteriophage beta consisting of seven markers has been constructed and compared with the vegetative map. The mapping system utilizes heteroimmune double lysogens and capitalizes on the fact that these double lysogens are very unstable and throw off monolysogenic segregants. The prophage map, produced by characterizing the recombinant phage in these monolysogenic segregants, appears to be a cyclic permutation of the vegetative map with the gene for toxin at one end of the prophage map and the gene for phage immunity at the other. This permutation is in accord with the Campbell model for insertion of lambda phage if a site between the toxin and immunity genes in the vegetative map is designated as the phage attachment site. The position of the gene for toxin in the prophage map suggests that converting phages may have originated as specialized transducing phages for this gene.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.9M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BARDSDALE WL, PAPPENHEIMER AM., Jr Phage-host relationships in nontoxigenic and toxigenic diphtheria bacilli. J Bacteriol. 1954 Feb;67(2):220–232. [PMC free article] [PubMed]
  • Calef E. Mapping of integration and excision crossovers in superinfection double lysogens for phage lambda in Escherichia coli. Genetics. 1967 Mar;55(3):547–556. [PMC free article] [PubMed]
  • FREEMAN VJ. Studies on the virulence of bacteriophage-infected strains of Corynebacterium diphtheriae. J Bacteriol. 1951 Jun;61(6):675–688. [PMC free article] [PubMed]
  • GROMAN NB. Evidence for the induced nature of the change from nontoxigenicity to toxigenicity in Corynebacterium diphtheriae as a result of exposure to specific bacteriophage. J Bacteriol. 1953 Aug;66(2):184–191. [PMC free article] [PubMed]
  • GROMAN NB. Evidence for the active role of bacteriophage in the conversion of nontoxigenic Corynebacterium diphtheriae to toxin production. J Bacteriol. 1955 Jan;69(1):9–15. [PMC free article] [PubMed]
  • GROMAN NB, BOBB D. The inhibition of adsorption of Corynebacterium diphtheriae phage by tween 80. Virology. 1955 Sep;1(3):313–323. [PubMed]
  • GROMAN NB, EATON M. Genetic factors in Corynebacterium diphtheriae conversion. J Bacteriol. 1955 Dec;70(6):637–640. [PMC free article] [PubMed]
  • GROMAN NB, EATON M, BOOHER ZK. Studies of mono- and polylysogenic Corynebacterium diphtheriae. J Bacteriol. 1958 Mar;75(3):320–325. [PMC free article] [PubMed]
  • Goff CG. Chemical structure of a modification of the Escherichia coli ribonucleic acid polymerase alpha polypeptides induced by bacteriophage T4 infection. J Biol Chem. 1974 Oct 10;249(19):6181–6190. [PubMed]
  • Holmes RK. Characterization and genetic mapping of nontoxinogenic (tox) mutants of corynebacteriophage beta. J Virol. 1976 Jul;19(1):195–207. [PMC free article] [PubMed]
  • Holmes RK, Barksdale L. Genetic analysis of tox+ and tox- bacteriophages of Corynebacterium diphtheriae. J Virol. 1969 Jun;3(6):586–598. [PMC free article] [PubMed]
  • Iglewski BH, Kabat D. NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin,. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2284–2288. [PMC free article] [PubMed]
  • Laird W, Groman N. Orientation of the tox gene in the prophage of corynebacteriophage beta. J Virol. 1976 Jul;19(1):228–231. [PMC free article] [PubMed]
  • Matsuda M, Kanei C, Yoneda M. Temperature-sensitive mutants on nonlysogenizing corynebacteriophage hv : their isolation, characterization and relation to toxiongenesis. Biken J. 1971 Jun;14(2):119–129. [PubMed]
  • Singer RA. Temperature-sensitive mutants of toxinogenic corynebacteriophage beta. I. Genetics. Virology. 1973 Oct;55(2):347–356. [PubMed]
  • Uchida T, Gill DM, Pappenheimer AM., Jr Mutation in the structural gene for diphtheria toxin carried by temperate phage . Nat New Biol. 1971 Sep 1;233(35):8–11. [PubMed]
  • Uchida T, Pappenheimer AM, Jr, Greany R. Diphtheria toxin and related proteins. I. Isolation and properties of mutant proteins serologically related to diphtheria toxin. J Biol Chem. 1973 Jun 10;248(11):3838–3844. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...