• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of jvirolPermissionsJournals.ASM.orgJournalJV ArticleJournal InfoAuthorsReviewers
J Virol. Jul 1978; 27(1): 38–44.
PMCID: PMC354138

Interaction between bacteriophage Sf6 and Shigella flexner.

Abstract

The Shigella flexneri phage Sf6 has an isometric head with hexagonal symmetry 53nm in diameter. The noncontractile tails in 16 nm long and terminates with a base plate containing six spikes. Sf6 is typical of the C phages in the morphological classification of Bradley. Phage Sf6 processes alpha-1,3-endorhamnosidase activity as demonstrated by methylation and reducing end group sugar analyses of the products obtained on interaction with the O-polysaccharide chain of S.flexneri strains which have the O-group 3,4 antigen. The major end product was an octasaccharide with the following structure: Rha III-GlcNAc-Rha I-Rha II-Rha III-GlcNAc-Rha I-Rha II. Acetylation of 0-2 of rhamnose III of the O-polysaccharide chain, either brought about by Sf6 lysogenization or found in wild-type S. flexneri (3b) strains, prevented enzymatic hydrolysis. O-deacetylation of the polysaccharide chain again made it susceptible to the S6f endorhamnosidase.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bradley DE. Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967 Dec;31(4):230–314. [PMC free article] [PubMed]
  • CASPAR DL, KLUG A. Physical principles in the construction of regular viruses. Cold Spring Harb Symp Quant Biol. 1962;27:1–24. [PubMed]
  • Dröge W, Lüderitz O, Westphal O. Biochemical studies on lipopolysaccharides of Salmonella R mutants. 3. The linkage of the heptose units. Eur J Biochem. 1968 Mar;4(1):126–133. [PubMed]
  • Gemski P, Jr, Koeltzow DE, Formal SB. Phage conversion of Shigella flexneri group antigens. Infect Immun. 1975 Apr;11(4):685–691. [PMC free article] [PubMed]
  • Kanegasaki S, Wright A. Studies on the mechanism of phage adsorption: interaction between phage epsilon15 and its cellular receptor. Virology. 1973 Mar;52(1):160–173. [PubMed]
  • Kenne L, Lindberg B, Petersson K, Katzenellenbogen E, Romanowska E. Structural studies of the Shigella flexneri variant X, type 5 a and type 5 b O-antigens. Eur J Biochem. 1977 Jun 15;76(2):327–330. [PubMed]
  • Kenne L, Lindberg B, Petersson K. Basic structure of the oligosaccharide repeating-unit of the Shigella flexneri O-antigens. Carbohydr Res. 1977 Jul;56(2):363–370. [PubMed]
  • Lindberg B, Lönngren J, Romanowska E, Rudén U. Location of O-acetyl groups in the Shigella flexneri types 3c and 4b lipopolysaccharides. Acta Chem Scand. 1972;26(9):3808–3810. [PubMed]
  • Wallenfels B, Jann K. The action of bacteriophage omega 8 on two strains of Escherichia coli 08. J Gen Microbiol. 1974 Mar;81(1):131–144. [PubMed]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...